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3D Reconstructions Using Charge Contrast Imaging

Published online by Cambridge University Press:  02 July 2020

Travis C Baroni ,
Brendan J Griffin  and
Frank J Lincoln
Travis C Baroni
Affiliation:
Department of Chemistry, The University of Western Australia, Nedlands, W.A.6907.
Brendan J Griffin
Affiliation:
Western Australian Centre for Microscopy And Microanalysis, The University of Western Australia, Nedlands, W.A.6907.
Frank J Lincoln
Affiliation:
Department of Chemistry, The University of Western Australia, Nedlands, W.A.6907.
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Extract

Three-dimensional reconstructions are a useful visualisation aid for understanding the volumetric relationships between features in an object. Typically, three dimensional reconstructions have evolved with regards to biological applications, where the collection and manipulation of the data has matured to a point where volumetric rendering has become almost routine. Two common data collection procedures involve either virtual sectioning using CLSM, where layers within the object are imaged successively without mechanical sectioning, or alternatively the projection of the object is imaged, via TEM while being rotated through a range of angles. This paper describes a novel application of three dimensional imaging by collecting 2 dimensional serial section images using a recently described technique for imaging materials using a VPSEM, known as Charge Contrast Imaging.

Figure 1 shows a typical image of a polished grain of gibbsite. Previous work has correlated the appearance of the concentric rings to laboratory controlled precipitation events, thus showing the technique provides information on the precipitation history

Type
Environmental Scanning Electron Microscopy and Other Wet Work
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 284 - 285
Copyright
Copyright © Microscopy Society of America

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References

1. Griffin, B.J. Microscopy & Microanalysis. (1997), 1197–1198.CrossRefGoogle Scholar

2. Roach, G.I.D.et al. Light Metals. (1998), 153–158.Google Scholar

3. Baroni, T.C., Griffin, B.J. and Lincoln, F.J. AMAS V. (1999), inpress.Google Scholar