Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-02T23:00:27.203Z Has data issue: false hasContentIssue false
  • English
  • Français

2G Alters the Utricular Macula of Chick Embryos

Published online by Cambridge University Press:  02 July 2020

Hirotaka Hara  and
César D. Fermin
Hirotaka Hara
Affiliation:
Department of Pathology. Tulane Medical School, New Orleans, La70112USA
César D. Fermin
Affiliation:
Department of Pathology. Tulane Medical School, New Orleans, La70112USA
Get access

Extract

Chick embryos (Gallus domesticus) exposed continuously from day one until 18 days of incubation to a 2G acceleration (hyperG chicks) force hatched with altered macular epithelia. Fertile eggs were placed in an incubator and exposed to 2G on a centrifuge by the original apparatus until 18 days of incubation. Effective 2G loads were obtained by rotating the apparatus at a constant velocity of 327.5 degree/sec with the incubator mounted at 55 cm from the center axis [1]. The vestibules of the newly hatch chicks were dissected and fixed in 2.5% glutaraldehyde. The utricles were rinsed in phosphate buffer, postfixed with 2% osmic acid and dehydrated in graded ethanol and embedded in plastic. Semithin sections were examined under Light microscopy with computerized video imaging system [2] and ultrathin sections were examined under TEM.

Type
Developmental Biology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1168 - 1169
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Hara, H., et al., Acta Otolaryngol (Stockh), 1995. 519(Suppl): p. 257–261.Google Scholar
2.Fermin, C. and DeGraw, S., J. Anat, 1995.186: p. 469–481.Google Scholar
3.Fermin, C et ah, Histol Histopath, 1996.11(2): p. 407–426.Google Scholar
4. Acknowledgement, The authors thank the Dept. of Pathology at Tulane, NASA and the NIH for supporting this work..Google Scholar