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The curious case of Cladonia luteoalba: no support for its distinction

Published online by Cambridge University Press:  13 December 2022

Ivana Černajová Open the ORCID record for Ivana Černajová [Opens in a new window] ,
Jana Steinová ,
Zuzana Škvorová  and
Pavel Škaloud
Ivana Černajová*
Affiliation:
Department of Botany, Faculty of Science, Charles University, Prague, Czechia Institute of Botany of the Czech Academy of Sciences, Průhonice, Czechia
Jana Steinová
Affiliation:
Department of Botany, Faculty of Science, Charles University, Prague, Czechia
Zuzana Škvorová
Affiliation:
Department of Botany, Faculty of Science, Charles University, Prague, Czechia
Pavel Škaloud
Affiliation:
Department of Botany, Faculty of Science, Charles University, Prague, Czechia
*
Author for correspondence: Ivana Černajová. E-mail: [email protected]
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Abstract

Cladonia luteoalba shows a specific pattern in chemical variability. Its chemotype coincides with that of the associated Cladonia thalli. This has led to the formation of various hypotheses, but its true nature has never been clarified. We collected C. luteoalba in Central Europe and Norway. The chemotypes were detected by TLC and the mycobionts and photobionts were identified by Sanger sequencing of ITS rDNA. Mycobiont cultures were obtained and Illumina metabarcoding of the fungal ITS1 rDNA region was performed targeting minor mycobionts within the thalli. None of the methods supported C. luteoalba as a distinct Cladonia species. In phylogenetic analyses, it was placed in C. straminea and the C. coccifera agg., following the pattern in chemistry. No minor Cladonia were detected by metabarcoding or cultivation. Thus, C. luteoalba remains enigmatic as our data did not support its distinction as a separate Cladonia species.

Keywords

Asterochlorischemotypeslichenmetabarcodingmycobiont culturephylogenySanger sequencing
Type
Standard Paper
Information
The Lichenologist , Volume 54 , Issue 6 , November 2022 , pp. 345 - 354
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the British Lichen Society

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