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A novel, green, and biocompatible graphene-based carbonaceous material for immobilization of cytochrome c

Published online by Cambridge University Press:  09 November 2018

Kuo Gai ,
Maoping Kang ,
Qian Huang ,
Sainan Zheng ,
Lei Zhang ,
Chaoliang Zhang  and
Liying Hao Open the ORCID record for Liying Hao [Opens in a new window]
Kuo Gai
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Maoping Kang
Affiliation:
Department of Mining Engineering, Shanxi Institute of Energy, Taiyuan 030600, People’s Republic of China
Qian Huang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Sainan Zheng
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Lei Zhang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Chaoliang Zhang
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
Liying Hao*
Affiliation:
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of China
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

The immobilization of cytochrome c (cyt c) on tea polyphenol functionalized and reduced graphene oxide (TPG) was carried out by a simple adsorption process. Intriguingly, TPG with large surface area exhibited excellent adsorption behaviors and good biocompatibility. The adsorbed materials were characterized by various methods including scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). And the effects of adsorption behavior of cyt c were discussed in detail. The results showed the adsorption behavior was dependent on the pH value and showed a high adsorption capacity as high as 1.414 × 104 mg/g and was friendly to normal cells (mouse fibroblast cell line, L929). In conclusion, we proposed the introduction of TPG as novel material and used the adsorption method to immobilize cyt c, which would provide a novel material and simple method for the enrichment of protein.

Keywords

TPGcytochrome cadsorption
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 24 , 28 December 2018 , pp. 4270 - 4277
Copyright
Copyright © Materials Research Society 2018 

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Footnotes

b)

These authors contributed equally to this work.

References

REFERENCES

Shang, W., Nuffer, J.H., Muñiz-Papandrea, V.A., Colón, W., Siegel, R.W., and Dordick, J.S.: Cytochrome c on silica nanoparticles: Influence of nanoparticle size on protein structure, stability, and activity. Small 5, 470 (2009).CrossRefGoogle ScholarPubMed
Li, J., Sun, R., Hao, C., He, G., Zhang, L., and Wang, J.: The behavior of the adsorption of cytochrome c on lipid monolayers: A study by the Langmuir–Blodgett technique and theoretical analysis. Biophys. Chem. 205, 33 (2015).CrossRefGoogle ScholarPubMed
Salehnia, F., Hosseini, M., and Ganjali, M.R.: A fluorometric aptamer based assay for cytochrome c using fluorescent graphitic carbon nitride nanosheets. Microchim. Acta 184, 2157 (2017).CrossRefGoogle Scholar
Yin, X., Cai, J., Feng, H., Wu, Z., Zou, J., and Cai, Q.: A novel VS2 nanosheet-based biosensor for rapid fluorescence detection of cytochrome c. New J. Chem. 39, 1892 (2015).CrossRefGoogle Scholar
Pandiaraj, M., Madasamy, T., Gollavilli, P.N., Balamurugan, M., Kotamraju, S., Rao, V.K., Bhargava, K., and Karunakaran, C.: Nanomaterial-based electrochemical biosensors for cytochrome c using cytochrome c reductase. Bioelectrochemistry 91, 1 (2013).CrossRefGoogle ScholarPubMed
Yang, D-H., Shin, M.J., Choi, S.M., Lee, C-S., and Shin, J.S.: Cytochrome c assembly on fullerene nanohybrid metal oxide ultrathin films. RSC Adv. 6, 19173 (2016).CrossRefGoogle Scholar
Liu, J-M. and Yan, X-P.: Ultrasensitive, selective and simultaneous detection of cytochrome c and insulin based on immunoassay and aptamer-based bioassay in combination with Au/Ag nanoparticle tagging and ICP-MS detection. J. Anal. At. Spectrom. 26, 1191 (2011).CrossRefGoogle Scholar
Cao, L., Li, X., Qin, L., Kang, S-Z., and Li, G.: Graphene quantum dots supported by graphene oxide as a sensitive fluorescence nanosensor for cytochrome c detection and intracellular imaging. J. Mater. Chem. B 5, 6300 (2017).CrossRefGoogle Scholar
Crouser, E.D., Gadd, M.E., Julian, M.W., Huff, J.E., Broekemeier, K.M., Robbins, K.A., and Pfeiffer, D.R.: Quantitation of cytochrome c release from rat liver mitochondria. Anal. Biochem. 317, 67 (2003).CrossRefGoogle ScholarPubMed
Hao, L., Song, H., Zhang, L., Wan, X., Tang, Y., and Lv, Y.: SiO2/graphene composite for highly selective adsorption of Pb(II) ion. J. Colloid Interface Sci. 369, 381 (2012).CrossRefGoogle ScholarPubMed
Vinu, A., Murugesan, V., Tangermann, O., and Hartmann, M.: Adsorption of cytochrome c on mesoporous molecular sieves: Influence of pH, pore diameter, and aluminum incorporation. Chem. Mater. 16, 3056 (2004).CrossRefGoogle Scholar
Washmon-Kriel, L., Jimenez, V.L., and Balkus, K.J.: Cytochrome c immobilization into mesoporous molecular sieves. J. Mol. Catal. B: Enzym. 10, 453 (2000).CrossRefGoogle Scholar
Patila, M., Pavlidis, I.V., Diamanti, E.K., Katapodis, P., Gournis, D., and Stamatis, H.: Enhancement of cytochrome c catalytic behaviour by affecting the heme environment using functionalized carbon-based nanomaterials. Process Biochem. 48, 1010 (2013).CrossRefGoogle Scholar
Feng, W. and Ji, P.: Enzymes immobilized on carbon nanotubes. Biotechnol. Adv. 29, 889 (2011).CrossRefGoogle ScholarPubMed
Song, H., Hao, L., Tian, Y., Wan, X., Zhang, L., and Lv, Y.: Stable and water-dispersible graphene nanosheets: Sustainable preparation, functionalization, and high-performance adsorbents for Pb2+. ChemPlusChem 77, 379 (2012).CrossRefGoogle Scholar
Zhang, X., Liang, Q., Han, Q., Wan, W., and Ding, M.: Metal-organic frameworks@graphene hybrid aerogels for solid-phase extraction of non-steroidal anti-inflammatory drugs and selective enrichment of proteins. Analyst 141, 4219 (2016).CrossRefGoogle ScholarPubMed
Lu, C-H., Yang, H-H., Zhu, C-L., Chen, X., and Chen, G-N.: A graphene platform for sensing biomolecules. Angew. Chem. 121, 4879 (2009).CrossRefGoogle Scholar
Novoselov, K.S., Geim, A.K., Morozov, S.V., Jiang, D., Zhang, Y., Dubonos, S.V., Grigorieva, I.V., and Firsov, A.A.: Electric field effect in atomically thin carbon films. Science 306, 666 (2004).CrossRefGoogle ScholarPubMed
Mei, Q., Chen, J., Zhao, J., Yang, L., Liu, B., Liu, R., and Zhang, Z.: Atomic oxygen tailored graphene oxide nanosheets emissions for multicolor cellular imaging. ACS Appl. Mater. Interfaces 8, 7390 (2016).CrossRefGoogle ScholarPubMed
Wang, X., Hao, L., Zhang, C., Chen, J., and Zhang, P.: High efficient anti-cancer drug delivery systems using tea polyphenols reduced and functionalized graphene oxide. J. Biomater. Appl. 31, 1108 (2017).CrossRefGoogle ScholarPubMed
Gollavelli, G. and Ling, Y.: Multi-functional graphene as an in vitro and in vivo imaging probe. Biomaterials 33, 2532 (2012).CrossRefGoogle Scholar
Wisitsoraat, A., Mensing, J., Karuwan, C., Sriprachuabwong, C., Jaruwongrungsee, K., Phokharatkul, D., Daniels, T., Liewhiran, C., and Tuantranont, A.: Printed organo-functionalized graphene for biosensing applications. Biosens. Bioelectron. 87, 7 (2017).CrossRefGoogle ScholarPubMed
Hao, L.Y., Wang, X.Q., Huang, Q., Liu, M., Zhang, C.L., Chen, J., Zhang, P., and Cai, X.X.: One step green reduced and functionalized graphene oxide for highly efficient loading and effectively release of doxorubicin hydrochloride. J. Biomed. Nanotechnol. 13, 1309 (2017).CrossRefGoogle Scholar
Hao, L.Y., Gai, K., Huang, Q., Liu, M.T., Sun, K., Fu, N., and Lin, Y.F.: Green and high-efficiency reduction of graphene oxide for highly loading drug to enhance cancer therapy. J. Biomed. Nanotechnol. 13, 1210 (2017).CrossRefGoogle Scholar
Huang, Q., Hao, L., Xie, J., Gong, T., Liao, J., and Lin, Y.: Tea polyphenol-functionalized graphene/chitosan as an experimental platform with improved mechanical behavior and bioactivity. ACS Appl. Mater. Interfaces 7, 20893 (2015).CrossRefGoogle ScholarPubMed
Hisanaga, A., Ishida, H., Sakao, K., Sogo, T., Kumamoto, T., Hashimoto, F., and Hou, D.: Anti-inflammatory activity and molecular mechanism of Oolong tea theasinensin. Food Funct. 5, 1891 (2014).CrossRefGoogle ScholarPubMed
Forester, S. and Lambert, J.: The role of antioxidant versus pro-oxidant effects of green tea polyphenols in cancer prevention. Mol. Nutr. Food Res. 55, 844 (2011).CrossRefGoogle ScholarPubMed
Butt, M. and Sultan, M.: Green tea: nature’s defense against malignancies. Crit. Rev. Food Sci. Nutr. 49, 463 (2009).CrossRefGoogle ScholarPubMed
Cho, H.S., Kim, S., Lee, S.Y., Park, J.A., Kim, S.J., and Chun, H.S.: Protective effect of the green tea component, L-theanine on environmental toxins-induced neuronal cell death. NeuroToxicology 29, 656 (2008).CrossRefGoogle ScholarPubMed
Rathore, K. and Wang, H.C.R.: Green tea catechin extract in intervention of chronic breast cell carcinogenesis induced by environmental carcinogens. Mol. Carcinog. 51, 280 (2012).CrossRefGoogle ScholarPubMed
Syed, D.N., Afaq, F., Kweon, M.H., Hadi, N., Bhatia, N., Spiegelman, V.S., and Mukhtar, H.: Green tea polyphenol EGCG suppresses cigarette smoke condensate-induced NF-κB activation in normal human bronchial epithelial cells. Oncogene 26, 673 (2007).CrossRefGoogle ScholarPubMed
Li, Y., Zhang, P., Du, Q., Peng, X., Liu, T., Wang, Z., Xia, Y., Zhang, W., Wang, K., Zhu, H., and Wu, D.: Adsorption of fluoride from aqueous solution by graphene. J. Colloid Interface Sci. 363, 348 (2011).CrossRefGoogle ScholarPubMed
Park, M., Park, S.S., Selvaraj, M., Kim, I., and Ha, C-S.: Hydrophobic periodic mesoporous organosilicas for the adsorption of cytochrome c. J. Porous Mater. 18, 217 (2011).CrossRefGoogle Scholar
Šimšíková, M. and Antalík, M.: Interaction of cytochrome c with zinc oxide nanoparticles. Colloids Surf., B 103, 630 (2013).CrossRefGoogle ScholarPubMed