Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-12-03T19:44:31.520Z Has data issue: false hasContentIssue false
  • English
  • Français

S-100 protein localization in minor salivary gland tumours: an aid to diagnosis

Published online by Cambridge University Press:  29 June 2007

J. B. Campbell ,
J Crocker  and
P. M. Shenoi
J. B. Campbell*
Affiliation:
Department of Otolaryngology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
J Crocker
Affiliation:
Department of Pathology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
P. M. Shenoi
Affiliation:
Department of Otolaryngology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
*
Department of Otolaryngology, The Queen Elizabeth Hospital, Queen Elizabeth Medical Centre, Edgbaston, Birmingham B15 2TH.
Get access Rights & Permissions [Opens in a new window]

Abstract

The clinician is almost entirely dependent on the histopathologist to accurately diagnose minor salivary gland tumours, but in some cases the histological interpretation of the specimen is very difficult. Recently it has been demonstrated using immunohistochemical techniques that S-100 protein is present in some salivary gland tissues and its localization has been used as an aid in the differentiation of major salivary gland tumours. To assess its value in the diagnosis of minor salivary gland tumours it was localized in sections from 15 such tumours using both a standard peroxidase-antiperoxidase (PAP) and a newly developed immunogold-silver staining sequence (IGSS) technique. Strong staining for S-100 protein was seen in the nuclei and cytoplasm of the cellular areas and also in the cells in the chondroid and myxoid areas of pleomorphic adenomas. Generally the staining was more intense and widespread with the IGSS method. No staining was observed in any of the other tumour types. We conclude that S-100 protein localization is a valuable aid in the differentiation of minor salivary gland tumours. Furthermore, the IGSS method enables more sensitive ‘reading’ of the staining reaction.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 102 , Issue 10 , October 1988 , pp. 905 - 908
Copyright
Copyright © JLO (1984) Limited 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agnantis, N. T. and Rosen, P. P. (1979) Mammary carcinoma with osteoclast-like giant cells. A study of eight cases with follow-up data. American Journal of Clinical Pathology, 72: 383–389.CrossRefGoogle ScholarPubMed
Balogh, K., Wolbarsht, R. L., Federman, M. and O'Hara, C. J. (1985) Carcinoma of the parotid gland with osteoclast-like giant cells. Immunohistochemcial and ultrastructural observations. Archives of Pathology and Laboratory Medicine, 109: 756–761.Google Scholar
Baniel, J., Konichezky, M. and Wolloch, Y. (1987) Osteoclast-type giant cell tumour of the pancreas. Case report. Acta Chi-rurgica Scandinavica 153: 67–69.Google Scholar
Berendt, R. C, Shnitka, T. K., Wiens, E., Manickavel, V. and Jewell, L. D. (1987) The osteoclast-type giant cell tumour of the pancreas. Archives of Pathology and Laboratory Medicine, 111; 43–48.Google Scholar
Chambers, J. J. and Horton, M. A. (1984) Osteoclasts: putative, surrogate and authentic. Journal of Pathology, 144: 295–296.CrossRefGoogle ScholarPubMed
Eusebi, V., Martin, S. A., Govoni, E. and Rosai, J. (1984) Giant cell tumour of major salivary glands: Report of three cases, one occurring in association with a malignant mixed tumour. American Journal of Clinical Pathology, 81: 666–675.CrossRefGoogle Scholar
Hui, K. J., Batsakis, J. G., Luna, M. A., Mackay, B. and Byers, R. M. (1986) Salivary duct adenocarcinoma: a high grade malignancy. Journal of Laryngology and Otology, 100: 105–114.CrossRefGoogle ScholarPubMed
Jeffrey, I., Crow, J. and Ellis, B. W. (1983) Osteoclast-type giant cell tumour of the pancreas. Journal of Clinical Pathology, 36: 1165–1170.CrossRefGoogle ScholarPubMed
Leader, M., Patel, J., Collins, M. and Henry, K. (1987) Anti-al-antichymotrypsin staining of 194 sarcomas, 38 carcinomas, and 17 malignant melanomas. Its lack of specificity as a tumour marker. American Journal of Surgical Pathology, 11: 133–139.CrossRefGoogle ScholarPubMed
Ling, L., Klein, M. J., Sissons, H. A. and Steiner, G. C. (1986) Lysozyme and al-antitrypsin in giant cell tumour of bone and in other lesions that contain giant cells. Archives of Pathology and Laboratory Medicine. 110: 713–718.Google Scholar
Marder, R. J., Variakojis, D., Silver, J. and Epstein, A. L. (1985) Immunohistochemical analysis of human lymphomas and mono-clonal antibodies to B cell and la antigens reactive in paraffin sections. Laboratory Investigations, 52: 497–504.Google Scholar
Ordonez, N. G., Khorsand, J., Ayala, A. G., andSneige, N. (1986) Oat cell carcinoma of the urinary tract. An immunohistochemi-cal and electron microscopic study. Cancer, 58: 2519–2530.3.0.CO;2-0>CrossRefGoogle ScholarPubMed
Rosai, J. (1968) carcinoma of pancreas simulating giant cell tumour of bone. Electron-microscopic evidence of its acinar cell origin. Cancer, 22: 333–344.Google Scholar
Tsukada, T., Tippens, D., Gordon, D., Ross, A. and Gown, A. M. (1984) HHF35, a muscle actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. American Journal of Pathology, 126: 51–61.Google Scholar
Underwood, J. C. E. (1984) From where comes the osteoclast? Journal of Pathology, 144: 225–226.CrossRefGoogle ScholarPubMed
Wittekind, C. H., Wachner, R., Henke, W. and von Kleist, S. (1986) Localization of CEA, HCG, lysozyme, alpha-1-antitryp-sin, and alpha-1-antichymotrypsin in gastric cancer and prognosis. Virchows Archives (A), 409: 715–724.CrossRefGoogle ScholarPubMed