Learning Objectives:
The Clinic is operating annually over 100 choleasteatomas (655 operations in the years 2010 -2015). Because of a common bacterial infection a bacteriologic analysis indicates for Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus as the most commonly detected in middle ear infection.
Having in mind a literature suggestion of a partial analogy between oncogenesis and cholesteatoma formation and own experience in identification of oncogenes and tumor suppressor genes modulating progression of laryngeal cancer we have undertaken a molecular analysis targeting for an identification of genetic backgroung of cholesteatoma. Array-CGH scanning of a genome indicated for frequent gains and losses of gene copy number in the genome. The results will be further analyzed to identify amplified regions potentially indicating location of oncogenes and homozygous deletions covering loci oftumor suppressor genes involved in cholesteatoma.
Independently a molecular cytogenetic technique was applied to analyze 8q24 chromosome region to estimate an amplification and potential rearrangement(s) of c-Myc oncogene. Fluorescent in situ Hybridization (FISH) with the use of specific DNA probes (regular fluorescent, break a part) is being applied.
The results will be presented during the meeting.
