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Electrophoretic differentiation of soluble egg antigens from Schistosoma mansoni isolates using SDS-PAGE

Published online by Cambridge University Press:  05 June 2009

D. Pillay
D. Pillay
Affiliation:
Department of Microbiology, University of Durban-Westville, Durban, South Africa
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Abstract

In order to detect intraspecies differences among isolates of Schistosoma mansoni from different geographical regions, soluble egg antigens (SEAs) of three isolates were characterized using SDS-PAGE. SEAs were prepared from eggs of a South African (SA), Eastern Caprivian (EC) and Puerto Rican (PR) isolate, separated by SDS-PAGE, stained and the molecular masses of constituent polypeptides determined. Protein profiles differed for all three isolates with the number of polypeptides varying from 25 for the PR isolate to 27 for the EC and SA isolates. Molecular masses of the polypeptides fall within the range of 12.0 kDa to 97.9 kDa. The EC and SA isolates shared ten common polypeptides, the PR and SA isolates five and the PR and EC isolates two. Only a 30.8 kDa and a 12.0 kDa polypeptide were common to all three isolates. These differences may, to some extent, account for regional variations in the morbidity of intestinal schistosomiasis reported for different geographical isolates of S. mansoni.

Type
Research Note
Information
Journal of Helminthology , Volume 70 , Issue 1 , March 1996 , pp. 91 - 93
Copyright
Copyright © Cambridge University Press 1996

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References

Boros, D.L., Tomford, R. & Warren, K.S. (1977) Induction of granulomatous and elictation of cutaneous sensitivity by partially purified soluble egg antigen of Schistosoma mansoni. Journal of Immunology 118, 373–376.CrossRefGoogle Scholar
Carter, C.E. & Colley, D.G. (1978) An electrophoretic analysis of Schistosoma mansoni soluble egg antigen preparation. Journal of Parasitology 64, 385–390.CrossRefGoogle ScholarPubMed
Colley, D.G. (1983) Schistosome antigen laboratory workshop: Summary of a meeting sponsored by the Edna McConnell Clark Foundation. Journal of Parasitology 69, 45–48.CrossRefGoogle ScholarPubMed
Dettman, C.D., Higgins-Opitz, S.B. & Bronner, G.N. (1987) A comment on the taxonomic status and correct nomenclature of laboratory colonies of Mastomys. South African Journal of Science 83,395.Google Scholar
Dettman, C.D., Higgins-Opitz, S.B. & Saikoolal, A. (1989) Enhanced efficacy of the paddling method for schistosome infection of rodents by a four step pre-soaking procedure. Parasitology Research 76, 183–184.CrossRefGoogle ScholarPubMed
Dunne, D.W., Lucas, S., Bickle, Q., Pearson, S., Madgwick, L., Bain, J. & Doenhoff, M.J. (1981) Identification and partial purification of an antigen (Wl) from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 54–71.CrossRefGoogle Scholar
Higgins-Opitz, S.B. (1991) Aspects of the host-parasite relationships of two Southern African isolates of Schistosoma mansoni: A comparative experimental study. PhD Thesis, University of Natal, Durban, South Africa.Google Scholar
Higgins-Opitz, S.B. & Dettman, C.D. (1991) The infection characteristics of a South African isolate of Schistosoma mansoni: a comparison with a Puerto Rican isolate in BALB/c mice and Mastomys coucha. Parasitology Research 77,142–151.CrossRefGoogle Scholar
Higgins-Opitz, S.B., Bhoola, K.D. & Dettman, C.D. (1987) Mastomys coucha as a host for experimental schistosomiasis. I. Some pathological responses to Schistosoma mansoni. South African Journal of Science 82, 696–699.Google Scholar
Jackson, T.F.H.G., Dettman, C.D. & Higgins-Opitz, S.B. (1982) Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents. Laboratory Animals 16, 65–67.CrossRefGoogle ScholarPubMed
Laemmli, U.K. & Favre, M. (1973) Maturation of the head of bacteriophage T4. DNA packaging events. Journal of Molecular Biology 80, 579–599.CrossRefGoogle ScholarPubMed
Lowry, O.K., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Lukacs, N.W. & Boros, D.L. (1991) Identification of larval cross-reactive and egg-specific antigens involved in granuloma formation in murine schistosomiasis mansoni. Infection and Immunity 59, 3237–3242.CrossRefGoogle ScholarPubMed
Moore, D.V., Yolles, T.K. & Meleney, H.E. (1949) A comparison of common laboratory animals as experimental hosts for Schistosoma mansoni. Journal of Parasitology 35,156–170.CrossRefGoogle ScholarPubMed
Pillay, D. (1995) Electrophoretic differentiation of Schistosoma mansoni soluble egg antigens. Proceedings of the Year of Louis Pasteur International Symposium, Etiology and Pathogenesis of Infectious Diseases, Dakar, Senegal 1, 158.Google Scholar
Pillay, D. & Pillay, B. (1994a) Random amplified polymorphic DNA analysis shows intrasperies variation among Schistosoma mansoni isolates. Medical Science Research 22, 369–371.Google Scholar
Pillay, D. & Pillay, B. (1994b) Schistosoma mansoni: PCR amplification shows intraspecific variation among geographical isolates. Experimental Parasitology 79, 57–58.CrossRefGoogle ScholarPubMed
Tendler, M., Klinkert, M., Diogo, C.M. & Savino, W. (1990) Molecular characterisation of an extract of Schistosoma mansoni with protease activity. Journal of Immunology and Immunopharmacology 10, 214–215.Google Scholar
Versnel, J.M. (ed.) (1988) The South African index of laboratory animals. 3rd edn.Johannesburg, MRC Laboratory Animal Unit.Google Scholar