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Identification and pathogenicity of Pratylenchus scribneri on tomato in Sichuan Province of People's Republic of China

Published online by Cambridge University Press:  10 January 2022

Y.H. Xia ,
J. Li ,
M.R. Sun ,
B. Lei ,
H.L. Li ,
Y. Li Open the ORCID record for Y. Li [Opens in a new window]  and
K. Wang
Y.H. Xia
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
J. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
M.R. Sun
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
B. Lei
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
H.L. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
Y. Li
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
K. Wang
Affiliation:
College of Plant Protection, Henan Agricultural University, Zhengzhou, People's Republic of China National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, People's Republic of China
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Abstract

Root-lesion nematodes (Pratylenchus spp.) are a group of economically important pathogens that have caused serious economic losses in many crops. In 2019, root-lesion nematodes were recovered from tomato (Solanum lycopersicum) root samples collected from Sichuan Province, People's Republic of China (PRC). Extracted nematodes were disinfected, and one individual female was cultured on a carrot disc for propagation at 25 °C by parthenogenesis and designated the SC isolate. Afterwards, the isolate was identified on the basis of morphometric and molecular markers. Both morphometric characters and molecular analysis of the internal transcribed spacer region gene (ITS) of ribosomal DNA, the D2-D3 expansion region of the 28S rDNA gene and the mitochondrial cytochrome oxidase I (mtDNA-COI) gene revealed that the species of root-lesion nematode was Pratylenchus scribneri. The Bayesian tree inferred from the ITS rDNA, 28S rDNA and mtDNA-COI gene sequences also showed that this isolate formed a highly supported clade with other P. scribneri isolates. The pathogenicity of the root-lesion nematode SC isolate on tomato was assessed, showing that tomato was a suitable host for P. scribneri. To the best of our knowledge, this is the first report of P. scribneri on tomato in Sichuan Province, PRC. These are also the first molecular data obtained from P. scribneri on tomato in the PRC, and the pathogenicity of P. scribneri to tomato was studied for the first time. This study provides scientific data for the detection, identification and control of tomato root-lesion nematode disease.

Keywords

IdentificationpathogenicityPratylenchus scribneritomatoroot-lesion nematode
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e5
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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References

Andrés, MF, Pinochet, J, Hernandez, BA and Delibes, A (2000) Detection and analysis of inter- and intraspecific diversity of Pratylenchus spp. using isozyme markers. Plant Pathology 49(5), 640–649.CrossRefGoogle Scholar
Byrd, DW, Kirkpatrick, T and Barker, KR (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of Nematology 15(1), 142–143.Google Scholar
Castillo, P and Vovlas, N (2007) Pratylenchus (Nematoda: Pratylenchus): Diagnosis, biology, pathogenicity and management. Nematology monographs and perspectives, Vol. 6. Leiden, The Netherlands, Brill.CrossRefGoogle Scholar
De Ley, P, Felix, MA, Frisse, LM and Steven, AN (1999) Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology 1(6), 591–612.CrossRefGoogle Scholar
De Luca, F, Fanelli, E, Divito, M, Reyes, A and De, GC (2004) Comparison of the sequences of the D3 expansion of the 26S ribosomal genes reveals different degrees of heterogeneity in different populations and species of Pratylenchus from the Mediterranean region. European Journal of Plant Pathology 110(9), 949–957.CrossRefGoogle Scholar
Diao, Y, Larsen, M, Kamvar, ZN, Zhang, C and Liu, XL (2019) Genetic differentiation and clonal expansion of Chinese Botrytis cinerea populations from tomato and other crops in China. Phytopathology 110(2), 428–439.CrossRefGoogle ScholarPubMed
Flis, L, Dobosz, R, Rybarczyk, MLK, Wasilewska-Nascimento, B, Kubicz, M and Winiszewska, G (2018) First report of the lesion nematodes: Pratylenchus brachyurus and Pratylenchus delattrei on tomato (Solanum lycopersicum L.) plants in CCape Verde. Helminthologia 55(1), 88–94.CrossRefGoogle ScholarPubMed
Freckman, DW and Caswell, EP (1985) The ecology of nematodes in agroecosystems. Annual Review of Phytopathology 23(1), 275–296.CrossRefGoogle Scholar
Gondal, AS, Ijaz, M, Riaz, K and Khan, A (2012) Effect of different doses of fungicide (Mancozeb) against Alternaria leaf blight of tomato in tunnel. Journal of Plant Pathology and Microbiology 21(3), 1–3.Google Scholar
Goo, MYC and Sipes, BS (1997) Host preference of Radopholus citrophilus from Hawaiian anthurium among selected tropical ornamentals. Hort Science 32(7), 1237–1238.Google Scholar
Hahn, ML, Sarah, JL, Boisseau, M, Vines, NJ, Wright, DJ and Burrows, PR (2010) Reproductive fitness and pathogenicity of selected Radopholus populations on two banana cultivars. Plant Pathology 45(2), 1–9.Google Scholar
Handoo, ZA, Skantar, AM, Kantor, MR, Hafez, SL and Hult, MN (2020) Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al. 2007), from California. Journal of Nematology 52, 1–5.Google Scholar
Hooper, DJ, Hallman, J and Subbotin, SA (2005) Methods for extraction, processing and detection of plant and soil nematodes. pp. 53–86 In Luc, M, Sikora R, A, Bridge, J (Eds) Plant parasitic nematodes in subtropical and tropical agriculture. 2nd ed. Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Hou, SC (2001) Introduction of several root rot nematodes on crops in Liaoning Province. Journal of Liaoning Agricultural Technical College 3, 12–14.Google Scholar
Huelsenbeck, JP and Ronquist, F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17(8), 754–755.CrossRefGoogle ScholarPubMed
Janssen, T, Karssen, G, Couvreur, M, Waeyenberge, L and Bert, W (2017) The pitfalls of molecular species identification: A case study within the genus Pratylenchus (Nematoda: Pratylenchidae). Nematology 19(10), 1179–1199.CrossRefGoogle Scholar
Jones, MGK and Fosu-Nyarko, J (2014) Molecular biology of root lesion nematodes (Pratylenchus spp.) and their interaction with host plants. Annals of Applied Biology 164(2), 163–181.CrossRefGoogle Scholar
Jones, JT, Haegeman, A, Danchin, EG, et al. (2013) Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14(9), 946–961.CrossRefGoogle ScholarPubMed
Kaplan, DT and Davis, EL (1990) Improved nematode extraction from carrot disk culture. Journal of Nematology 22(3), 399–406.Google ScholarPubMed
Li, SM, Feng, ZX and Xu, KL (1985) Study on the nematodes of on the crops in Henan Province. Acta Agricultural Universitatis Henanensis 85, 257–269.Google Scholar
Li, Y, Wang, S, Liu, YK, Lu, QS, Wang, K and Li, HL (2019) Occurrence of soybean root rot caused by Pratylenchus coffeae in Henan Province, China. Plant Disease 103(6), 1435–1435.CrossRefGoogle Scholar
Liu, XY and Liu, WZ (2007) Species of nematodes of Pratylenchidae in Shandong province. Journal of Qingdao Agricultural University 24, 175–176.Google Scholar
Liu, HL, Wang, X, Li, HM, Li, YX, Xue, BW and Ma, JK (2018) Molecular identification of Pratylenchus species in 10 samples collected from wheat field in Huanghuai Region of China. Scientia Agricultura Sinica 51, 2898–2912.Google Scholar
Loof, PAA (1978) The genus Pratylenchus Filipjev, (1936) (Nematoda: Pratylenchidae): a review of its anatomy, morphology, distribution, systematics and identification. Sveriges Landbruksuniversiteit Växtskyddsrapporter 5, 1–12.Google Scholar
Nguyen, TD, Le, T, Nguyen, HT, Nguyen, T, Liebanas, G and Trinh, QP (2017) Morphological and molecular characteristics of Pratylenchus haiduongensis sp. n., a new species of root-lesion nematodes associated with carrot in Vietnam. Journal of Nematology 49(3), 276–285.Google Scholar
Nicol, JM, Turner, SJ, Coyne, DL, Den, NL, Hockland, S and Maafi, ZT (2001) Current nematode threats to world agriculture. pp. 21–43 In Jones, J, Gheysen, G, Fenoll, C (Eds) Genomics and molecular genetics of plant–nematode interactions. Dordrecht, The Netherlands, Springer.Google Scholar
Nylander, JA (2004) Mrmodeltest 2.3. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University.Google Scholar
Rebois, RV and Huettel, RN (1986) Population dynamics, root penetration, and feeding behavior of Pratylenchus agilis in monoxenic root cultures of corn, tomato, and soybean. Journal of Nematology 18(3), 392–397.Google ScholarPubMed
Reise, RW, Huettel, RN and Sayre, RM (1987) Carrot callus tissue for culture of endoparasitic nematodes. Journal of Nematology 19(3), 387–389.Google ScholarPubMed
Roman, J and Hirschmann, H (1969) Morphology and morphometrics of six species of pratylenchus. Journal of Nematology 1, 363.Google ScholarPubMed
Ryss, AY (2002) Genus Pratylenchus Filipjev: multientry and monoentry keys and diagnostic relationships (Nematoda: Tylenchida: Pratylenchidae). Zoosystematica Rossica 10(2), 241–255.Google Scholar
Seinhorst, JM (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4(1), 67–69.CrossRefGoogle Scholar
Shaner, G, Stromberg, EL and Lacy, GH (1992) Nomenclature and concepts of pathogenicity and virulence. Annual Review of Phytopathology 30, 47–66.CrossRefGoogle ScholarPubMed
Siddiqui, IA, Sher, SA and French, AM (1973) Distribution of plant parasitic nematodes in California. Sacramento, CA, USA, Department of Food and Agriculture, Division of Plant Industry. p. 324.Google Scholar
Subbotin, SA, Sturhan, D, Chizhov, VN, Vovlas, N and Baldwin, JG (2006) Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematology 8(3), 455–474.CrossRefGoogle Scholar
Talwana, H, Sibanda, Z, Wanjohi, W, Luambano-Nyoni, N, Massawe, C, Manzanilla-López, RH, Davies, KJ, Hunt, DJ and Sikora, RA (2016) Agricultural nematology in east and Southern Africa: Problems, management strategies and stakeholder linkages. Pest Management Science 72(2), 226–245.CrossRefGoogle Scholar
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M and Kumar, S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28(10), 2731–2739.CrossRefGoogle ScholarPubMed
Vignesh, K, Rajamohan, K, Balabaskar, P and Anandan, R (2021) In vitro efficacy of PGPR Pseudomonas fluorescens against Fusarium wilt of tomato caused by Fusarium oxysporum f.sp. Lycopersici. Plant Archives 21(1), 430–460.CrossRefGoogle Scholar
Wang, JL, Zhang, JC and Gu, JF (2011) DNA extracted method on single nematode. Plant Quarantine 25(1), 32–35.Google Scholar
Wang, K, Xie, H, Li, Y, Wu, WJ and Xu, CL (2016) Morphology and molecular analysis of Paratylenchus nanjingensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Pinus massoniana in China. Journal of Helminthology 90(2), 166–173.CrossRefGoogle Scholar
Wang, S, Liu, YK, Xia, YH, Hao, PH, Wang, K, Li, HL and Li, Y (2021) Identification and pathogenicity of a root-lesion nematode species on tobacco in Henan. Journal of Northwest A&F University 49(1), 85–93.Google Scholar
Xie, H (2005) Taxonomy of plant nematodes. 2nd ed. Beijing, Higher Education Press.Google Scholar