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The expression of propionicin PLG-1 gene (plg-1) by lactic starters1

Published online by Cambridge University Press:  16 February 2015

Sameh E Mohamed  and
Mahmoud K Tahoun
Sameh E Mohamed*
Affiliation:
Department of Food Technology, Arid Lands Cultivation Research Institute, City for Scientific Research and Technological Applications, Borg El-Arab, Egypt
Mahmoud K Tahoun
Affiliation:
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
*
*For correspondence; e-mail: [email protected]
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Abstract

Propionicin PLG-1 is a bacteriocin produced by Propionibacterium thoenii P127. Such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Yersinia enterocolitica and a strain of Corynebacterium sp. In the present study, plg-1 gene expressing propionicin PLG-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. The PCR product containing plg-1 gene was transferred to different lactic acid bacterial (LAB) strains using pLEB590 as a cloning vector to give the modified vector pLEBPLG-1. LAB transformants showed an antimicrobial activity against Esch. coli DH5α (most affected strain), Listeria monocytogenes 18116, and Salmonella enterica 25566 as model pathogenic strains. Such LAB transformants can be used in dairy industry to control the food-borne pathogens that are largely distributed worldwide and to feed schoolchildren in the poor countries where dangerous epidemic diseases and diarrhoea prevail.

Keywords

Propionicin PLG-1plg-1 genelactic acid bacteria (LAB)food-borne pathogens
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 2 , May 2015 , pp. 209 - 214
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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Footnotes

1

Presented in the 5th Congress of European Microbiologists (FEMS 2013, Leipzig, Germany) as a poster presentation.

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