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Biochemical changes in developing embryos of Schistocerca gregaria (Orthoptera: Acrididae) induced by pheromone produced by ovipositing gregarious females

Published online by Cambridge University Press:  01 July 2015

Fathiya M. Khamis ,
Paul O. Mireji ,
Ellie O. Osir ,
Mabel O. Imbuga  and
Ahmed Hassanali
Fathiya M. Khamis*
Affiliation:
The International Centre of Insect Physiology and Ecology (icipe), PO Box 30772, Nairobi 00100, Kenya Department of Biochemistry and Biotechnology, Kenyatta University, PO Box 43844, Nairobi00100, Kenya
Paul O. Mireji
Affiliation:
Department of Biochemistry and Molecular Biology, Egerton University, PO Box 536-20115, Egerton, Kenya
Ellie O. Osir
Affiliation:
The International Centre of Insect Physiology and Ecology (icipe), PO Box 30772, Nairobi 00100, Kenya
Mabel O. Imbuga
Affiliation:
Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, PO Box 62000, Nairobi, Kenya
Ahmed Hassanali
Affiliation:
The International Centre of Insect Physiology and Ecology (icipe), PO Box 30772, Nairobi 00100, Kenya Department of Chemistry, Kenyatta University, PO Box 43844, Nairobi00100, Kenya
*
*E-mail: [email protected]
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Abstract

Trans-generational transfer of gregarious-phase traits in the desert locust Schistocerca gregaria (Forskål, 1775) is mediated by primer gregarizing pheromonal signals produced by ovipositing females that experience crowding. We monitored time-course proteomic events in eggs from solitary-reared locusts that had been exposed for 1, 3, 5, 7, 10 and 12 days to different levels of the sand-associated gregarizing signal originating from 0, 3, 5 or 10 ovipositions by crowd-reared females. Evidence for the phase transition was sought by comparing the protein patterns of embryos thus exposed with those from crowd-reared (gregarious) controls; this comparison was continued until the stage of the first instars. Expressed proteins were analysed by two-dimensional protein gel electrophoresis, and patterns from the different treatments within stages were compared by profile matching and χ2 analyses. Eggs derived from crowd- and solitary-reared females showed essentially similar protein patterns at early stages of embryogenesis; however, mature stages (particularly, days 10 and 12) and hatchlings demonstrated significantly different patterns. Protein patterns of eggs from solitary-reared females that were incubated in sand contaminated with the pheromonal signal and of the hatchlings that emerged were similar to those derived from gregarious females and dependent on the level of the pheromone to which the embryos had been exposed. The results confirm the gregarizing effect of the signal and constitute a useful basis for unravelling the mechanism of the signalling cascades associated with gene expressions triggered by the pheromone.

Keywords

Schistocerca gregariaprimer gregarizing pheromonephase transition2D protein patterns
Type
Research Papers
Information
International Journal of Tropical Insect Science , Volume 35 , Issue 3 , September 2015 , pp. 125 - 131
Copyright
Copyright © ICIPE 2015 

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