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Monitoring Rotavirus Environmental Contamination in a Pediatric Unit Using Polymerase Chain Reaction

Published online by Cambridge University Press:  02 January 2015

Hervé Soule ,
Odile Genoulaz ,
Bénédicte Gratacap-Cavallier ,
Marie Reine Mallaret ,
Patrice Morand ,
Patrice François ,
Dominique Luu Duc Bin ,
Agnés Charvier ,
Cécile Bost-Bru  and
Jean-Marie Seigneurin
Hervé Soule*
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Odile Genoulaz
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Bénédicte Gratacap-Cavallier
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Marie Reine Mallaret
Affiliation:
Unité d'Hygiene Hospitalière, CHU Grenoble, CH Annecy, France
Patrice Morand
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Patrice François
Affiliation:
Département de Pédiatric, CHU Grenoble, CH Annecy, France
Dominique Luu Duc Bin
Affiliation:
Unité d'Hygiene Hospitalière, CHU Grenoble, CH Annecy, France
Agnés Charvier
Affiliation:
Service des Maladies Infectieuses, CH Annecy, France
Cécile Bost-Bru
Affiliation:
Département de Pédiatric, CHU Grenoble, CH Annecy, France
Jean-Marie Seigneurin
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
*
Laboratoire de Virologie, CHU Grenoble, BP217, 38043 Grenoble Cedex 9, France
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Abstract

Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 20 , Issue 6 , June 1999 , pp. 432 - 434
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1999

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