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A technique for replica plating Coprinus lagopus, a filamentous fungus

Published online by Cambridge University Press:  14 April 2009

Philippa J. Kaplan  and
D. Walls
Philippa J. Kaplan
Affiliation:
Department of Botany and Microbiology, University College London
D. Walls
Affiliation:
Department of Botany and Microbiology, University College London
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Summary

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The problem of replica plating filamentous fungi such as Coprinus lagopus is overcome by inducing micro-colonies with sodium deoxycholate and using ‘Velcro’, a hooked material, to replace velveteen in the standard replica plating technique. ‘Velcro’ is advantageous in that it has a regular pattern of closely spaced hooks which transfer small inocula from the colonies on the master plate.

Type
Short Papers
Information
Genetics Research , Volume 17 , Issue 3 , June 1971 , pp. 279 - 281
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Lederberg, J. & Lederberg, E. M. (1952). Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63, 399.CrossRefGoogle ScholarPubMed
Lewis, D. (1961). Genetical analysis of methionine suppressors in Coprinus lagopus. Genetical Research 2, 141.CrossRefGoogle Scholar
Mackintosh, M. E. & Pritchard, R. H. (1963). The production and replica plating of micro-colonies of Aspergillus nidulans. Genetical Research 4, 320–322.CrossRefGoogle Scholar
Maling, B. (1960). Replica plating and rapid ascus collection of Neurospora. Journal of General Microbiology 23, 257.CrossRefGoogle ScholarPubMed
Roberts, C. F. (1959). A replica plating tecnhique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans). Journal of General Microbiology 20, 540.CrossRefGoogle Scholar
Tatum, E. L., Barratt, R. W. & Cutter, B. M. Jr., (1959). Chemical induction of colonial paramorphs in Neurospora and Syncephalastrum. Science, N.Y. 109, 509.CrossRefGoogle Scholar