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Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell culture

Published online by Cambridge University Press:  14 April 2009

David N. Radin  and
Peter S. Carlson
David N. Radin
Affiliation:
Department of Genetics, University of California, Berkeley, CA 94720, U.S.A.
Peter S. Carlson
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, U.S.A.
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Summary

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The herbicides Bentazone and Phenmedipharm kill the leaves of intact tobacco plants but do not affect callus cultures. Tolerant mutants were isolated by treating leaves of previously γ-irradiated haploid plants with herbicide then excising and culturing the green herbicide-resistant cell clones on the otherwise yellowed leaves. Among plants subsequently regenerated were a total of ten stable independently isolated mutants. Sexual crosses show these ten represent four Bentazone and two Phenmedipharm loci; all mutants were recessive to wild type.

Type
Research Article
Information
Genetics Research , Volume 32 , Issue 1 , August 1978 , pp. 85 - 89
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Atkinson, P. H. & Mathews, R. E. F. (1970). On the origin of dark green tissue in tobacco leaves infected with tobacco mosaic virus. Virology 40. 344–356.CrossRefGoogle ScholarPubMed
Burk, L. G. & Menser, H. T. (1964). A dominant aurea mutation in tobacco. Tobacco Science 8, 101–104.Google Scholar
Carlson, P. S. (1973). Methionine sulfoximine-resistant mutants of tobacco. Science 180, 1366–1368.CrossRefGoogle ScholarPubMed
Carlson, P. S. & Polacco, J. C. (1975). Plant cell culture techniques: genetic aspects of crop improvement. Science 188, 622–625.CrossRefGoogle Scholar
Carlson, P. S., Smith, H. H. & Dearing, R. D. (1972). Parasexual interspecific plant hybridization. Proceedings of the National Academy of Sciences, U.S.A. 69, 2292–2294.CrossRefGoogle ScholarPubMed
Day, P. R. (1977). Plant genetics: Increasing crop yield. Science 197, 1334–1339.CrossRefGoogle ScholarPubMed
Day, P. R., Carlson, P. S., Gomberg, O. L., Jaworski, E. G., Meretzki, A., Nelson, O. E., Sussex, I. M. & Torrey, J. G. (1977). Somatic cell genetic manipulation in plants. BioScience 27, 116–118.CrossRefGoogle Scholar
Linsmaier, E. M. & Skoog, F. (1965). Organic growth factor requirements of tobacco tissue cultures. Physiologia Plantarum 18, 100–127.CrossRefGoogle Scholar
Reinert, J. & Bajaj, Y. P. S. (1977). Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. New York: Springer-Verlag.Google Scholar
Rice, T. B. & Carlson, P. S. (1975). Genetic analysis and plant improvement. Annual Reviews of Plant Physiology 26, 279–308.CrossRefGoogle Scholar