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Characteristics of some single-step mutants to chloramphenicol resistance in Escherichia coli K12 and their interactions with R-factor genes

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, Edinburgh 9
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Six one-step Chloramphenicol (Cm)-resistant mutants of Escherichia coli K12 were graded for resistance to Cm, Tetracycline (Tc) and Puromyein (Pm) by streaking on minimal agar plates containing antibiotic. They fell into at least three distinct groups on the basis of their resistance patterns. One mutant showed increased sensitivity to Pm. Most of the mutants expressed their effect on resistance to Cm and Tc in the presence of R-factors carrying resistance genes for these antibiotics, but one mutant with a relatively high level of resistance to Cm had its resistance effect completely masked in the presence of R-mediated resistance. Similar cases were found among mutants selected for Cm-resistance in another strain of K12.

Type
Short Papers
Copyright
Copyright © Cambridge University Press 1966

References

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