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Polar Lippia extracts as alternatives for the postharvest control of Guazatine®-resistant strains of Penicillium digitatum in citrus

Published online by Cambridge University Press:  01 April 2009

Emmanuel Shikanga ,
Thierry Regnier ,
Sandra Combrinck  and
Ben Botha
Emmanuel Shikanga
Affiliation:
Dep. Chem., Fac. Sci., Tshwane Univ. Technol., PO Box 56208, Arcadia Pretoria 0001, Repub. S. Afr.
Thierry Regnier
Affiliation:
Dep. Chem., Fac. Sci., Tshwane Univ. Technol., PO Box 56208, Arcadia Pretoria 0001, Repub. S. Afr.
Sandra Combrinck
Affiliation:
Dep. Chem., Fac. Sci., Tshwane Univ. Technol., PO Box 56208, Arcadia Pretoria 0001, Repub. S. Afr.
Ben Botha
Affiliation:
Dep. Chem., Fac. Sci., Tshwane Univ. Technol., PO Box 56208, Arcadia Pretoria 0001, Repub. S. Afr.
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Abstract

Introduction. Penicillium digitatum is a commercially important postharvest pathogen of citrus that is responsible for significant annual global losses. Strains of the fungus, which exhibit strong resistance to widely used synthetic fungicides, are of major concern to the industry. The aim of the study was to investigate the antifungal activities of polar extracts and compounds from Lippia species, indigenous to South Africa, against a Guazatine®-resistant strain of P. digitatum.Materials and methods. In vitro tests were done by incorporating the compounds and plant extracts into Malt Extract Agar at concentrations ranging from (0.2 to 1.0) mg·mL–1. An in vivo (curative) assay was conducted using the checkerboard technique on ‘Valencia’ oranges. Results and discussion. A strong correlation between the in vitro and in vivo results was observed. All the compounds and extracts were able to inhibit fungal growth at concentrations above 0.6 mg·mL–1. Verbascoside was the most active compound, while extracts of Lippia javanica and Lippia rehmannii caused significant inhibition of mycelial growth. The observed activity was largely ascribed to the presence of verbascoside in the plant extracts. Conclusion. Polar extracts of Lippia species have potential as environmentally friendly alternatives for the control of P. digitatum on citrus.

Keywords

South AfricaCitrusPenicillium digitatumdisease controlpostharvest controlbiological controlLippiaplant extractsAfrique du SudCitrusPenicillium digitatumcontrôle de maladieslutte après récoltelutte biologiqueLippiaextrait d'origine végétale
Type
Research Article
Information
Fruits , Volume 64 , Issue 2 , March 2009 , pp. 75 - 82
Copyright
© CIRAD, EDP Sciences, 2009

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References

Plaza, P., Torres, R., UsaIl, J., Larmaca, N., Viñasi, I., Evaluation of the potential of commercial postharvest application of essential oils to control citrus decay, J. Hortic. Sci. Biotechnol. 79 (6) (2004) 935–940. CrossRef
Eckert, J.W., Ogawa, J.M., The chemical control of postharvest diseases: subtropical and tropical fruits, Annu. Rev. Phytopathol. 23 (1985) 421–454. CrossRef
Win, N.K.K., Jitareerat, P., Kanlayanarat, S., Sangchote, S., Effects of cinnamon extract, chitosan coating, hot water treatment and their combination on crown rot disease and quality of banana fruit, Postharvest Biol. Technol. 45 (3) (2007) 333–340. CrossRef
Eckert, J.W., Sievert, J.R., Ratnayake, M., Reduction of imazalil® effectiveness against citrus green mold in California packinghouses by resistant biotypes of Penicillium digitatum, Plant Dis. 78 (1994) 791–794. CrossRef
Aked, J., Khan, S.H., Magan, N., Control of anthracnose pathogens of banana (Colleto-trichum musae) using antioxidants alone and in combination with thiabendazole or imazalil, Plant Pathol. 50 (2001) 601–608.
Bajpai, V.K., Rahman, A., Kang, C.S., Chemical composition and antifungal properties of essential oils and crude extracts of Metasequoia glyptostroboides Miki ex Hu, Indian Crop. Prod. 26 (1) (2007) 28–35. CrossRef
Saks, Y., Barkai-Golan, R., Aloe vera gel activity against plant pathogenic fungi, Postharvest Biol. Technol. 6 (1995) 159–165. CrossRef
Mari, M., Bertolini, P., Pratella, G.C., Non-conventional methods for the control of postharvest pear diseases, J. Appl. Microbiol. 94 (2003) 761–766. CrossRef
Regnier, T., Du Plooy, W., Combrinck, S., Botha, B., Fungitoxicity of Lippia scaberrima and selected terpenoid components on two mango postharvest spoilage pathogens, Postharvest Biol. Technol. 48 (2008) 254–258. CrossRef
Nielsen, P.V., Rios, R., Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in packaging, with special emphasis on mustard essential oil, Int. J. Food Microbiol. 60 (2000) 219–229. CrossRef
Pascual, M.E, Siowing, K., Carretero, E, Sànchez Mata, D., Villar, A., Lippia: traditional uses, chemistry and pharmacology: a review, J. Ethnopharmacol. 76 (2001) 201–214. CrossRef
Combrinck S., Bosman A.A, Botha B.M., Du Plooy W., McCrindle R.I., Retief E., Effects of post-harvest drying on the essential oil and glandular trichomes of Lippia scaberrima Sond., J. Essent. Oil Res. 18 (2006) 80–84. CrossRef
Pieroni, A., Pachaly, P., Huang, Y., Van Poel, B., Vlietinck, A.J., Studies on anti-complementary activity of isolated flavones from Ugustrum vulgare and Phillyrea latifolia leaves (Oleaceae), J. Ethnopharmacol. 70 (2000) 213–217. CrossRef
Herbert, J.M., Mafrand, J.P., Toaubi, K., Augereau, J.M., Fouraste, I., Gleye, J., Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C, J. Nat. Prod. 54 (6) (1991) 1595–1600. CrossRef
Pennacchio, M., Traditional Australian aboriginal bush medicines, HerbGram 65 (2005) 38–44.
Owen, R.W., Haubner, R., Mier, W., Giacosa, A., Hull, W.E., Spiegelhalder, B., Bartsch, H., Isolation, structure elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes, Food Chem. Toxicol. 41 (2003) 703–717. CrossRef
Combrinck S., Chemical constituents of Lippia scaberrima Sond. (Verbenaceae), Tshwane Univ. Technol., Thesis, Pretoria, South Africa, 2006, 258 p. (available from libserv5.tut.ac.za:7780/pls/eres/wpg, acces-sed: 2008/10/07).
Tsai, T.-R., Tseng, T.-Y., Chien, C.-F., Tsai, T.-U., Identification and determination of geniposide contained in Gardenia jasminoides and two preparations of mixed traditional Chinese medicines, J. Chromatogr. 961 (1) (2002) 83–88. CrossRef
Eloff, J.N., A sensitive and quick microplate method to determine minimum inhibitory concentration of plant extracts for bacteria, Planta Med. 64 (1998) 711–714. CrossRef
Rojas, G., Levaro, J., Tortoriello, J., Navarro, V., Antimicrobial evaluation of certain plants used in Mexican traditional medicine for treatment of respiratory diseases, J. Ethno-pharmacol. 74 (2001) 97–101. CrossRef
Korsten L., Biological control of avocado fruit disease, Pretoria Univ., Thesis, Pretoria, South Africa, 1993, 213 p.
Frum Y., Viljoen A.M., Van Heerden F.R., Verbascoside and luteolin-5-glucoside isolated from Halleria lucida L. exhibit antagonistic anti-oxidant properties in vitro, S. Afr. J. Bot. 73 (4) (2007) 583–587.
De Andrade Lima C.S., De Amorim E.L.C., De Sena K.R., De Andrade Chiappeta A., Nunes X.P., Da-Cunha M.E.V.L., Da Silva M.S., Barbosa-Filho J.M., Antimicrobial activity of a mixture of two isomeric phenylpropanoid glycosides from Arrabidaea harleyi A.H. Gentry (Bignoniaceae), Braz. J. Pharm. Sci. 39 (2003) 77–81.