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Differential patterns of whole-genome DNA methylation in institutionalized children and children raised by their biological parents

Published online by Cambridge University Press:  29 November 2011

Oksana Yu. Naumova ,
Maria Lee ,
Roman Koposov ,
Moshe Szyf ,
Mary Dozier  and
Elena L. Grigorenko
Oksana Yu. Naumova
Affiliation:
Yale University Vavilov Institute of General Genetics RAS
Maria Lee
Affiliation:
Yale University
Roman Koposov
Affiliation:
University of Tromsø
Moshe Szyf
Affiliation:
McGill University
Mary Dozier
Affiliation:
University of Delaware
Elena L. Grigorenko*
Affiliation:
Yale University Moscow State University
*
Address correspondence and reprint requests to: Elena L. Grigorenko, Child Study Center, Yale University, 230 South Frontage Road, New Haven, CT 06519-1124; E-mail: [email protected].
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Abstract

Previous studies with nonhuman species have shown that animals exposed to early adversity show differential DNA methylation relative to comparison animals. The current study examined differential methylation among 14 children raised since birth in institutional care and 14 comparison children raised by their biological parents. Blood samples were taken from children in middle childhood. Analysis of whole-genome methylation patterns was performed using the Infinium HumanMethylation27 BeadChip assay (Illumina), which contains 27,578 CpG sites, covering approximately 14,000 gene promoters. Group differences were registered, which were characterized primarily by greater methylation in the institutionalized group relative to the comparison group, with most of these differences in genes involved in the control of immune response and cellular signaling systems, including a number of crucial players important for neural communication and brain development and functioning. The findings suggest that patterns of differential methylation seen in nonhuman species with altered maternal care are also characteristic of children who experience early maternal separation.

Type
Regular Articles
Information
Development and Psychopathology , Volume 24 , Issue 1 , February 2012 , pp. 143 - 155
Copyright
Copyright © Cambridge University Press 2012

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