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The effects of basic fibroblast growth factor in an animal model of acute mechanically induced right ventricular hypertrophy

Published online by Cambridge University Press:  27 January 2012

Vladimiro L. Vida ,
Arben Dedja ,
Elisabetta Faggin ,
Simone Speggiorin ,
Massimo A. Padalino ,
Giovanna Boccuzzo ,
Paolo Pauletto ,
Annalisa Angelini ,
Ornella Milanesi  and
Gaetano Thiene
...Show all authors
Vladimiro L. Vida*
Affiliation:
Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Padua, Italy
Arben Dedja
Affiliation:
Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Padua, Italy
Elisabetta Faggin
Affiliation:
Department of Clinical and Experimental Medicine, University of Padua, Padua, Italy
Simone Speggiorin
Affiliation:
Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Padua, Italy
Massimo A. Padalino
Affiliation:
Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Padua, Italy
Giovanna Boccuzzo
Affiliation:
Department of Statistics, University of Padua, Padua, Italy
Paolo Pauletto
Affiliation:
Department of Clinical and Experimental Medicine, University of Padua, Padua, Italy
Annalisa Angelini
Affiliation:
Department of Medical Diagnostic Sciences and Special Therapies, University of Padua, Padua, Italy
Ornella Milanesi
Affiliation:
Department of Paediatric Cardiology, University of Padua, Padua, Italy
Gaetano Thiene
Affiliation:
Department of Medical Diagnostic Sciences and Special Therapies, University of Padua, Padua, Italy
Giovanni Stellin
Affiliation:
Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Padua, Italy
*
Correspondence to: Dr V. L. Vida, MD, PhD, Department of Paediatric and Congenital Cardiac Surgery Unit, University of Padua, Via Giustiniani, 2-35128 Padua, Italy. Tel: +39 049 8212410; Fax: +39 049 8212409; E-mail: [email protected]
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Abstract

Objective

To evaluate the effect of a continuous infusion of basic fibroblast growth factor on the adaptive potential of the right ventricular myocardium after 30 days of mechanically induced overload in rats.

Materials and methods

We banded the pulmonary trunk, so as to increase the systolic workload of the right ventricle, in six Lewis/HanHsd rats at the age of 11 weeks, using six adult rats as controls. The six adult rats were also banded and received an additional continuous infusion of basic fibroblastic growth factor, using six rats with a continuous infusion of basic fibroblastic growth factor only as controls. We analysed the functional adaptation and structural changes of the right ventricular myocardium, blood vessels, and interstitial tissue 30 days after the increased afterload.

Results

The pulmonary artery banding induced an increase in the right ventricular free wall thickness of banded rats when compared with controls, which was mainly justified by an increase in cardiomyocyte area and in the percentage of extracellular fibrosis. The infusion of basic fibroblastic growth factor promotes a more extensive capillary network in banded rats (p < 0.001), which modulates the compensatory response of the right ventricle, promoting the hypertrophy of contractile elements and limiting the areas in which fibrosis develops (p < 0.001).

Conclusions

The subcutaneous infusion with osmotic pumps was a valid and reproducible method of delivering basic fibroblast growth factor to heart tissue. This infusion contributed to better preserve the right ventricular capillary network, hampering the development of interstitial fibrosis.

Keywords

Cardiac failureventricular remodellingcongenital defectsextracellular matrix
Type
Original Article
Information
Cardiology in the Young , Volume 22 , Issue 4 , August 2012 , pp. 436 - 442
Copyright
Copyright © Cambridge University Press 2012

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