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Insulin and Serum-stimulated Hexose Transport in In Vitro Aged Cultured Human Skin Fibroblasts

Published online by Cambridge University Press:  29 November 2010

Ralph J. Germinario ,
Maureen Oliveira  and
Susannia Manuel
Ralph J. Germinario
Affiliation:
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis—Jewish General Hospital, Montreal, Quebec
Maureen Oliveira
Affiliation:
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis—Jewish General Hospital, Montreal, Quebec
Susannia Manuel
Affiliation:
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis—Jewish General Hospital, Montreal, Quebec
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Abstract

Insulin (0.67 μM) and serum (10% v/v) stimulated hexose transport did not change with in vitro ageing of cultured human skin fibroblasts. Additionally, dexamethasone amplification of the insulin-stimulated response which yielded responses to near physiological concentrations of insulin (i.e. 6.7 nM), showed no changes with in vitro ageing. Basal (i.e. nonstimulated) hexose transport, either in the presence or absence of dexamethasone, did not change with in vitro ageing. The in vitro loss of proliferative potential during in vitro ageing of cultured human fibroblasts probably does not involve changes in short term insulin responses (e.g. stimulation of hexose transport).

Résumé

Le transport de l'hexose stimulé par l'insuline 0,67 μM et le sérum (10% v/v) n'a pas été modifié par le vieillissement in vitro des fibroblastes du tissu cutané humain. De plus, l'amplification par la dexaméthasone de la réponse stimulée par l'insuline qui a abouti à des réponses aux concentrations d'insuline voisines du niveau physiologique (c'est-à-dire non stimulé) de l'hexose, que ce soit en présence ou en l'absence de la dexaméthasone, n'a pas changé dans l'expérience du vieillissement in vitro. La perte du pouvoir prolifératif au cours de l'expérience du vieillissement in vitro des fibroblastes du tissu cutané humain n'entraîne probablement pas de modifications dans les réponses à court terme à l'insuline (c'est-à-dire dans la stimulations du transport de l'hexose).

Keywords

aging fibroblastshexose transportinsulin effect and serum effect
Type
Articles
Information
Canadian Journal on Aging / La Revue canadienne du vieillissement , Volume 5 , Issue 3 , Autumn/L'automne 1986 , pp. 157 - 164
Copyright
Copyright © Canadian Association on Gerontology 1986

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References

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