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Comparison of lipid-free haemoglobin and stroma-contaminated haemoglobin diets for adults of Stomoxys calcitrans (L.) (Diptera: Muscidae)

Published online by Cambridge University Press:  10 July 2009

G. Kapatsa ,
G. E. Spates ,
C. L. Sheffield  and
J. R. DeLoach
G. Kapatsa
Affiliation:
US Department of Agriculture, Agricultural Research Service, Veterinary Toxicology and Entomology Research Laboratory, P. O. Drawer GE, College Station, Texas 77841, USA
G. E. Spates
Affiliation:
US Department of Agriculture, Agricultural Research Service, Veterinary Toxicology and Entomology Research Laboratory, P. O. Drawer GE, College Station, Texas 77841, USA
C. L. Sheffield
Affiliation:
US Department of Agriculture, Agricultural Research Service, Veterinary Toxicology and Entomology Research Laboratory, P. O. Drawer GE, College Station, Texas 77841, USA
J. R. DeLoach
Affiliation:
US Department of Agriculture, Agricultural Research Service, Veterinary Toxicology and Entomology Research Laboratory, P. O. Drawer GE, College Station, Texas 77841, USA
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Abstract

Adults of Stomoxys calcitrans (L.) that fed on stroma-contaminated haemoglobin plus bovine serum albumin had normal survival, fecundity and egg hatch. Feeding on haemoglobin from which the erythrocyte membrane strome had been removed, together with bovine serum albumin, reduced fecundity to zero. The addition of membrane ghost preparation to a lipid-free haemoglobin and bovine serum albumin diet restored the essential nutrients for normal fly fecundity. For normal reproduction therefore, S. calcitrans adults require erythrocyte stroma.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 79 , Issue 1 , March 1989 , pp. 41 - 45
Copyright
Copyright © Cambridge University Press 1989

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References

Bridges, A. C. & Spates, G. E. (1983). Larval medium for the stable fly, Stomoxys calcitrans (L.).—Swest Ent. 8, 6–10.Google Scholar
DeLoach, J. R., Sheffield, C. L. & Spates, G. E. (1986). A continuous-flow high-yield process for preparation of lipid-free hemoglobin.—Analyt. Biochem. 157, 191–198.CrossRefGoogle ScholarPubMed
DeLoach, J. R. & Spates, G. E. (1984). Hemoglobin and albumin diet for adult Stomoxys calcitrans and Glossina palpalis.—Swest. Ent. 9, 28–34.Google Scholar
Ivie, G. W. & Wright, J. E. (1978). Fate of diflubenzuron in the stable fly and house fly.—J. agric. Fd Chem. 26, 90–94.CrossRefGoogle Scholar
Kabayo, J. P. & Taher, M. (1986). Effect of dialysing diet components on the reproductive physiology of G. p. palpalis.—J. Insect Physiol. 32, 543–548.CrossRefGoogle Scholar
Kabayo, J. P.Taher, M. & Van Der Vloedt, A. M. (1985). Development of a synthetic diet for Glossina (Diptera: Glossinidae).—Bull. ent. Res. 75, 635–640.CrossRefGoogle Scholar
Rabiner, S. F., Helbert, R. J., Lopas, H, & Freidman, L. H. (1967). Evaluation of a stroma-free hemoglobin solution for use as a plasma expander.—J. exp. Med. 126, 1127–1142.CrossRefGoogle ScholarPubMed
Riggs, A. (1981). Preparation of blood hemoglobins of vertebrates.—pp. 5–29in Antonini, E., Rossi-Bernardi, L. & Chiancone, E. (Eds). Methods in enzymology. Volume 76. Hemoglobins.—874 pp. Orlando, Florida, Academic Press.Google Scholar
Sheffield, C. L., Spates, G. E. & DeLoach, J. R. (in press). Hypoosmotic dialysis and ultrafiltration technique for preparation of mammalian hemoglobin: a comparision of three species.—Biomater. Artif. Cells Artif. Organs.Google Scholar
Sheffield, C. L., Spates, G. E., Droleskey, R. E., Green, R. & DeLoach, J. R. (1987). Preparation of lipid-free human hemoglobin by dialysis and ultrafiltration.—Biotechnol. Appl. Biochem. 9, 230–238.CrossRefGoogle ScholarPubMed