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Pathological changes in growing dogs fed on a balanced cassava (Manihot esculenta Crantz) diet

Published online by Cambridge University Press:  09 March 2007

Beryl P. Kamalu
Beryl P. Kamalu
Affiliation:
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Abstract

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Studies were carried out to determine the effects of the toxic principle linamarin, a cyanogenic glucoside, in a diet containing cassava (Manihot esculenta Crantz) in the form of gari fed to growing dogs for 14 weeks. There were three groups of dogs, each comprising six animals. One group was fed on a control diet with rice as the carbohydrate source, the second group was fed on cassava (gari) as the carbohydrate source and which was expected to release 10.8 mg HCN/kg cooked food, the third group was fed on the control diet to which enough NaCN was added at feeding time to release 10.8 mg HCN/kg cooked food in order to monitor the effects of the HCN released from gari. All diets contained 130 g erude protein (N × 6.25)/kg and were supplemented with vitamins and minerals. Each animal was given approximately 100 g diet/kg body weight for the duration of the experifnent. The biochemical variables investigated were plasma electrolytes, serum proteins, plasma-free amino acids, plasma enzymes and urine protein, and the histology of some metabolically active tissues, namely liver, kidney, myocardium, testis and adrenal gland, was studied. The gari diet caused an elevated plasma thiocyanate concentration (P < 0.01), elevated 24 h urinary thiocyanate excretion and elevated urinary protein excretion (P < 0.01), lowered serum albumin (P < 0.05), a plasma-free amino acid profile which resembled that found in kwashiorkor, lowered plasma K and Ca (P < 0.05). The rice + cyanide diet caused an elevated plasma thiocyanate (P < 0.01) and a 24 h urinary thiocyanate excretion that was significantly higher (P < 0.01) than that of the dogs fed on gari, but caused a urinary protein excretion that was significantly lower than that of the dogs fed on gari (P < 0.01), lowered serum albumin (P < 0.05), a plasma-free amino acid profile that indicated that the amino acids were not being utilized to the same extent as in the control (rice) group but were accumulating. Neither diet had an effect on plasma γ-glutamyltransferase (EC 2.3.2.2), alanine aminotransferase (EC2.6.1.2) or isocitrate dehydrogenase (EC 1.1.1.42) activities, plasma Na, Mg, and P concentrations. The gari diet caused generalized congestion and haemorrhage, periportal vacuolation of the liver, swelling, vacuolation and rupture of the epithelial cells of the proximal convoluted tubules of the kidney, myocardial degeneration and adrenal gland degeneration. In the testes there were occasional abnormal germ cells in the seminiferous tubules, and occasional seminiferous tubules denuded to basement membrane of germ cells but with remnants of Sertoli cells. Spermatogenesis, however, appeared to be normal since the percentage of round tubules in stage 8 of the spermatogenic cycle was not significantly different from that of the control dogs. The rice + cyanide diet caused nephrosis and a significantly reduced relative frequency of testicular tubules in stage 8 of the spermatogenic cycle (P < 0.01). There was also marked testicular germ cell sloughing and degeneration. There was adrenal gland hyperplasia and hypertrophy. It was concluded that the observed changes which occurred when the gari diet was consumed were not entirely due to cyanide.

Keywords

LinamarinPathological changesCassava (Manihot esculenta Crautz)Dog
Type
Effects of Cassava on Growing Dogs
Information
British Journal of Nutrition , Volume 69 , Issue 3 , May 1993 , pp. 921 - 934
Copyright
Copyright © The Nutrition Society 1993

References

Appleby, E. C. & Sohrabi, I. (1978). Pathology of the adrenal glands and paraganglia. Veterinary Record 102, 76–78.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (1970). Official Methods of Analysis, 11th ed., p. 438. Washington, DC: Association of Official Analytical Chemists.Google Scholar
Barrett, M. D. F., Hill, D. G., Alexander, J. C. & Zitnak, A. (1977). Fate of orally dosed linamarin in the rat. Canadian Journal of Physiology and Pharmacology 55, 134–136.Google Scholar
Bowler, R. O. (1944). The determination of thiocyanate in blood serum. Biochemical Journal 38, 385–386.Google Scholar
Chilaka, F. C. (1983). An investigation of the effects of high and prolonged cassava diets on some drug metabolizing enzymes of rat liver. MSc Thesis, University of Nigeria, Nsukka.Google Scholar
Conn, E. E. (1973). Cyanogenic glycosides. Agricultural and Food Chemistry 17, 519–526.CrossRefGoogle Scholar
Delange, F., Bourdoux, P., Colinet, E., Courtois, P., Hennart, P., Lagasse, R., Mafuta, P., Seghers, P., Thilly, C.,Vanderpas, J., Yunga, Y. & Ermans, A. M. (1982). Nutritional factors involved in the goitrogenic action of cassava. In Cassava Toxkity rind Thyroid: Research and Public Health Issues. Proceeding.P of a Workshop, Ottawa International Development Research Centre Monograph 207e, pp. 17–26 [Delange, F. and Ahluwalia, R., editors]. Ottawa: International Development Research Centre.Google Scholar
Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics 11, 1–42.Google Scholar
Foote, R. H., Swierstra, E. E. & Hunt, W. L. (1972). Spermatogenesis in the dog. Anatomical Record 173, 341–352.CrossRefGoogle ScholarPubMed
Gornall, A. G., Bardawill, C. J. & David, M. M. (1949). Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177, 751–766.CrossRefGoogle ScholarPubMed
Grimble, R. F., Sawyer, M. B. & Whitehead, R. G. (1969). Time relationships between the elevation of serum amino acid ratio and changes in liver composition in malnourished rats. British Journal of Nutrition 23,879–888.CrossRefGoogle ScholarPubMed
Grimble, R. F. & Whitehead, R. G. (1969). The relationship between an elevated serum amino acid ratio and the development of other biological abnormalities in the experimentally malnourished pig. British Journal of Nuitrition 23, 791–804.CrossRefGoogle ScholarPubMed
Grimble, R. F. & Whitehead, R. G. (1970 a). Changes in the concentration of specific amino acids in the serum of experimentally malnourished pigs. British Journal of Nutrition 24, 557–564.CrossRefGoogle ScholarPubMed
Grimble, R. F. & Whitehead, R. G. (1970 b). Fasting serum-amino acid patterns in kwashiorkor and after administration of different levels of protein. Lancet i, 918–921.Google Scholar
Hill, D. C. (1977). Physiological and biochemical responses of rats given potassium cyanide or linamarin. Cassuva as an Animal Feed. Proceedings of a Workshop held at University of Guelph, 1977. International Development Research Centre Monograph 095e, pp. 33–42 [Nestel, B. and Graham, M., editors]. Ottawa, Canada: International Development Research Centre.Google Scholar
Kamalu, B. P. (1991). The effect of a nutritionally balanced cassava diet on endocrine function using the dog as a model. 1. Pancreas. British Journal of Nutrition 65, 365–372.Google Scholar
Kamalu, B. P. & Agharanya, J. G. (1991). The effect of a nutritionally balanced cassava (Manihot esculentu Crantz) diet on endocrine function using the dog as a model. 2. Thyroid. British Journal of Nutrition 65.373–379.Google Scholar
Massey, V. & Alberty, R. A. (1954). Ionisation constants of fumarase. Biochimica et Biophysica Acta 13, 354–359.Google Scholar
Millward, D. J., Garlick, F. J., Nnanyelugo, D. O. & Waterlow, J. C. (1976). The relative importance of muscle protein synthesis and breakdown in the regulation of muscle mass. Biochemical Journal 156, 185–188.CrossRefGoogle ScholarPubMed
Nickerson, M. (1970). Antihypertensive agents and the drug therapy of hypertension. In The Pharmacological Basis of Therupeutics, 4th ed., pp. 735–736 [Goodman, L. S. and Gilman, A., editors]. New York: Macmillan.Google Scholar
Ononogbu, I. C. & Emole, I. (1978). The effect of gari on rat plasma cholesterol. Atherosclerosis 31, 101–104.Google Scholar
Philbrick, D. J., Hill, D. C. & Alexander, J. C. (1977). Physiological and biochemical changes associated with linamarin administration to rats. Toxicology and Pharmacology 42, 539–551.Google Scholar
Pullman, T. N. & McClure, W. W. (1954). The renal tubular reabsorption of thiocyanate in normal man. Journal of Laboratory and Clinical Medicine 43, 815–823.Google Scholar