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Evaluation of the cobalt requirement of beef cattle based on vitamin B12, folate, homocysteine and methylmalonic acid

Published online by Cambridge University Press:  09 March 2007

G. I. Stangl ,
F. J. Schwarz ,
H. Müller  and
M. Kirchgessner
G. I. Stangl*
Affiliation:
Institute of Nutrition Sciences, University of Technology of Munich, 85350 Freising-Weihenstephan, Germany
F. J. Schwarz
Affiliation:
Institute of Nutrition Sciences, University of Technology of Munich, 85350 Freising-Weihenstephan, Germany
H. Müller
Affiliation:
Institute of Nutrition Sciences, University of Technology of Munich, 85350 Freising-Weihenstephan, Germany
M. Kirchgessner
Affiliation:
Institute of Nutrition Sciences, University of Technology of Munich, 85350 Freising-Weihenstephan, Germany
*
*Corresponding author: Dr G. I. Stangl, fax +49 8161 715367, email [email protected]
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Abstract

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This investigation was designed to estimate the Co requirement of growing cattle on the basis of plasma and liver levels of vitamin B12 and folate, plasma levels of homocysteine and methylmalonic acid (MMA) and haematological variables. For this purpose thirty-four male intact cattle of the German Simmental breed (236 kg) were assigned randomly to ten groups and were fed corn silage-based diets which contained 70, 90, 109, 147, 184, 257, 327, 421, 589 or 689 μg Co/kg DM for 40 weeks. One-slope broken-line model analysis and a quadratic model with plateau were used to estimate the Co requirement. The broken-line model estimated the dietary Co requirement of growing cattle to be 257 (SE 29) ΜG/KG DIETARY DM BASED ON PLASMA VITAMIN B12 AS RESPONSE CRITERION. THE DIETARY CO LEVELS NEEDED TO MAXIMISE THE LIVER VITAMIN B12 AND LIVER FOLATE WERE 236 (se 8) and 190 (se 8) μg/kg dietary DM respectively. Plasma folate did not show any response to the different Co levels. The dietary Co was inversely correlated with the plasma concentrations of homocysteine and MMA. Estimates of the dietary Co concentration required to minimise homocysteine were 161 (se 10) μg/kg DM. When MMA was used as response criterion, the linear model yielded a Co requirement of 124 (se 3) μg/kg dietary DM. The quadratic model did not provide a better closeness of regression fit and yielded similar requirements to the linear model. Haemoglobin concentration and haematocrit tended to have a slight response to increasing dietary Co and were only decreased in cattle on diets containing less than 100 μg Co/kg DM. On the basis of the present data, recommended levels of dietary Co for normal folate metabolism and minimum homocysteine and MMA levels can be set to be 150–200 μg/kg DM; for maximum vitamin B12 levels, the desired Co content in the diet seems to be 250 μg/kg DM.

Keywords

Cobalt requirementCattle
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 5 , November 2000 , pp. 645 - 653
Copyright
Copyright © The Nutrition Society 2000

References

Andrews, ED (1965) Observations on the thrift of young sheep on a marginally cobalt-deficient area.New Zealand Journal of Agricultural Research 8, 788–817.CrossRefGoogle Scholar
Andrews, ED, Stephenson, BJ, Anderson, JP and Faithful, WC (1958) The effect of length of pastures on cobalt-deficiency disease in lambs.New Zealand Journal of Agricultural Research 31, 125–139.CrossRefGoogle Scholar
Coma, J, Carrion, D and Zimmerman, DR (1995) Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs.Journal of Animal Science 73, 472–481.CrossRefGoogle ScholarPubMed
Cornwell, PE, Morgan, SL and Vaughn, WH (1993) Modification of a high-performance liquid chromatographic method for assay of homocysteine in human plasma.Journal of Chromatography 617, 136–139.CrossRefGoogle ScholarPubMed
Duncan, IF, Greetree, PL and Ellisk, J (1986) Cobalt deficiency in cattle.Australian Veterinary Journal 63, 127–128.CrossRefGoogle ScholarPubMed
Filmer, JF and Underwood, EJ (1937) Enzootic marasmus. Further data concerning the potency of cobalt as a curative and prophylactic agent.Australian Veterinary Journal 13, 57–64.CrossRefGoogle Scholar
Gesellschaft fur Ernahrungsphysiologie(1995) Empfehlungen zur Energie- und Nährstoffversorgung der Mastrinder (Energy and nutrient requirements of beef cattle).Frankfurt (Main):DLGVerlag.Google Scholar
Hedrich, MF, Elliot, JM and Lowe, JE (1973) Response in vitamin B-12 production and absorption to increasing cobalt intake in the sheep.Journal of Nutrition 103, 1646–1651.CrossRefGoogle ScholarPubMed
Kawashima, T, Henry, PR, Bates, DG, Ammerman, CB, Littell, RC and Price, J (1997) Bioavailability of cobalt sources for ruminants: In vitro ruminal production of vitamin B-12 and total corrinoids in response to different cobalt sources and concentrations.Nutrition Research 17, 975–987.CrossRefGoogle Scholar
Kennedy, DG, O'Harte, FPM, Blanchflower, WJ and Rice, DA (1990) Development of a specific radioimmunoassay for vitamin B12 and its application in the diagnosis of cobalt deficiency in sheep.Veterinary Research Communications 14, 255–265.CrossRefGoogle ScholarPubMed
Kennedy, DG, Young, PB, Kennedy, S, Scott, JM, Molloy, AM, Weir, DG and Price, J (1995) Cobalt vitamin B12 deficiency and the activity of methylmalonyl CoA mutase and methionine synthase in cattle.International Journal for Vitamin and Nutrition Research 65, 241–247.Google ScholarPubMed
Kirchgessner, M, Schwarz, FJ and Stangl, GI (1998) Growth performance of beef cattle fed corn silage-based rations without Cu, Zn, Mn, Co and Se supplementation.Journal of Animal Physiology and Animal Nutrition 78, 141–153.CrossRefGoogle Scholar
Lowry, SR (1992) Use and misuse of multiple comparisons in animal experiments.Journal of Animal Science 70, 1971–1977.CrossRefGoogle ScholarPubMed
Marston, HR (1952) Cobalt, copper and molybdenum in the nutrition of animals and plants.Physiology Reviews 32, 66–121.CrossRefGoogle ScholarPubMed
McMurray, CH, Blanchflower, WJ, Rice, DA and McLoughlin, MF (1986) Sensitive and specific gas chromatographic method for the determination of methylmalonic acid in the plasma and urine of ruminants.Journal of Chromatography 378, 201–207.CrossRefGoogle ScholarPubMed
McNaught, KJ (1948) The cobalt content of North Island pastures.New Zealand Journal of Science and Technology A20, 14–30.Google Scholar
Mburu, JN, Kamau, JM and Badamana, MS (1993) Changes in serum levels of vitamin B12, feed intake, liveweight and hematological parameters in cobalt deficient small east African goats.International Journal for Vitamin and Nutrition Research 63, 135–139.Google ScholarPubMed
Mitchell, PJ, McOrist, S, Thomas, KW and McCausland, IP (1982) White liver disease of sheep.Australian Veterinary Journal 58, 181–184.CrossRefGoogle ScholarPubMed
Musewe, VO and Gombe, S (1980) Plasma vitamin B12 and reproductive performance of cows on cobalt-deficient pastures in the Rift Valley of Kenya.International Journal for Vitamin and Nutrition Research 50, 272–282.Google ScholarPubMed
National Research Council (1980) Mineral tolerance of domestic animals. Washington,DC: National Academy of Sciences.Google Scholar
National Research Council(1996) Nutrient requirements of beef cattle. Washington, DC: National Academy Press.Google Scholar
O'Harte, FPM, Kennedy, DG, Blanchflower, WJ and Rice, DA (1989) Methylmalonic acid in the diagnosis of cobalt deficiency in barley-fed lambs.British Journal of Nutrition 62, 729–738.CrossRefGoogle ScholarPubMed
Paragon, BM (1993) Readjustment of the supply of cobalt in the ruminant: critical approach.Recueil de Medecine Veterinaire de l'Ecole d'Alfort 169, 759–761.Google Scholar
Paterson, JE and MacPherson, A (1990) A comparison of serum vitamin B12 and serum methylmalonic acid as diagnostic measures of cobalt status in cattle.Veterinary Record 126, 329–332.Google ScholarPubMed
Poole, DBR, Fleming, GA and Kiely, J (1972) Cobalt deficiency in Ireland – soil, plant and animal.Irish Veterinary Journal 26, 109–117.Google Scholar
Price, J, Barris, SG and Ueno, S (1991) Unusual behaviour of a vitamin B12-binder, transcobalamin 1 in cattle plasma.Proceedings of the Nutrition Society 51, 23.Google Scholar
Price, J, Ueno, S, Wood, SGM, Anke, DMeissner, C and Mills, F (1993) Recent developments in the assay of plasma Proceedings of the Nutrition Society 51,23Google Scholar
Rice, DA, McLoughlin, M, Blanchflower, WJ, McMurray, CH and Goodall, EA (1989) Sequential changes in plasma methylmalonic acid and vitamin B12 in sheep eating cobalt-deficient grass.Biological Trace Element Research 22, 153–164.CrossRefGoogle ScholarPubMed
Robbins, KR (1986) A method, SAS program and example for fitting the broken-line to growth data. University of Tenessee Agricultural Experiment Station Research Report no. 86-09,1 – 8.Google Scholar
Robbins, KR, Norton, HW and Baker, DH (1979) Estimation of nutrient requirements from growth data.Journal of Nutrition 109, 1710–1714.CrossRefGoogle ScholarPubMed
Schwarz, FJ, Kirchgessner, M and Beckenbauer, MA (1985) Zur Ermittlung von Mastleistungskriterien von Jungbullen im Laufstall bei Einzeltierfütterung – dargestellt anhand von Ergebnissen zur Maissilagefütterung mit und ohne Energieergänzung (Determination of parameters for growth performance of beef cattle by using electronically controlled feeders).Züchtungskunde 57, 141–150.Google Scholar
Schwarz, FJ, Kirchgessner, M and Stangl, GI (2000) Cobalt requirement of beef cattlefeed intake and growth at different levels of cobalt supply.Journal of Animal Physiology and Animal Nutrition 83, 121–131.CrossRefGoogle Scholar
Scott, JM and Weir, DG (1981) The methyl folate trap. A physiological response in man to prevent methyl group deficiency in kwashiorkor (methionine deficiency) and an explanation for folic-acid induced exacerbation of subacute combined degeneration in pernicious anaemia.Lancet 2, 337–340.CrossRefGoogle Scholar
Shane, B and Stokstad, EL (1985) Vitamin B12-folate interrelationships.Annual Reviews of Nutrition 5, 115–141.CrossRefGoogle ScholarPubMed
Singh, KK and Chhabra, A (1995) Effect of dietary cobalt on ruminal vitamin B-12 synthesis and rumen metabolites.Journal of Nuclear Agriculture and Biology 24, 112–116.Google Scholar
Smith, RM and Mertz, W (1987) Trace Elements in Human and Animal Nutrition pp.143–183.[Mertz, W, editor]. New York,NY: Academic Press.CrossRefGoogle Scholar
Somers, M and Gawthorne, JM (1969) The effect of dietary cobalt intake on the plasma vitamin B12 concentration of sheep.Australian Journal of Experimental Biology and Medical Science 47, 227–233.CrossRefGoogle ScholarPubMed
Stangl, GI, Schwarz, FJ, Jahn, B and Kirchgessner, M (2000) Cobalt-deficiency-induced hyperhomocysteinaemia and oxidative status of cattle.British Journal of Nutrition 83, 3–6.CrossRefGoogle ScholarPubMed
Stangl, GI, Schwarz, FJ and Kirchgessner, M (1998) Cobalt deficiency effect on trace elements, hormones and enzymes involved in energy metabolism of cattle.International Journal for Vitamin and Nutrition Research 69, 120–126.CrossRefGoogle Scholar
Stangl, GI, Schwarz, FJ and Kirchgessner, M (1998) Moderate long-term cobalt-deficiency affects liver, brain and erythrocyte lipids and lipoproteins of cattle.Nutrition Research 19, 415–427.CrossRefGoogle Scholar
Sutton, AL and Elliot, JM (1972) Effect of ratio of roughage to concentrate and level of feed intake on ovine ruminal vitamin B12 production.Journal of Nutrition 102, 1341–1346.CrossRefGoogle Scholar
Ubbink, JB, Vermaak, WJH and Bissbort, S (1991) Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum.Journal of Chromatography 565, 441–446.CrossRefGoogle ScholarPubMed