Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-30T15:55:47.155Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of micronutrient supplementation on performance and epigenetic status in dairy cows

Published online by Cambridge University Press:  11 June 2020

M. Gasselin ,
M. Boutinaud ,
A. Prézelin ,
P. Debournoux ,
M. Fargetton ,
E. Mariani ,
J. Zawadzki ,
H. Kiefer  and
H. Jammes Open the ORCID record for H. Jammes [Opens in a new window]
M. Gasselin
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
M. Boutinaud
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
A. Prézelin
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
P. Debournoux
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
M. Fargetton
Affiliation:
INRAE, AGROCAMPUS Ouest PEGASE, 35590Saint-Gilles, France
E. Mariani
Affiliation:
XR-Repro, 43700Coubon, France
J. Zawadzki
Affiliation:
Pilardière Group®, 85590Saint-Mars-la-Réorthe, France
H. Kiefer
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
H. Jammes*
Affiliation:
Université Paris-Saclay, UVSQ, INRAE, BREED, 78350Jouy-en-Josas, France Ecole Nationale Vétérinaire d’Alfort, BREED, 94700Maisons-Alfort, France
*
†E-mail: [email protected]
Get access

Abstract

The postpartum period is crucial in dairy cows and is marked by major physiological and metabolic changes that affect milk production, immune response and fertility. Nutrition remains the most important lever for limiting the negative energy balance and its consequences on general health status in highly selected dairy cows. In order to analyze the effect of a commercial micronutrient on intrinsic parameters, performances and the epigenome of dairy cows, 2 groups of 12 Holstein cows were used: 1 fed a standard diet (mainly composed of corn silage, soybean meal and non-mineral supplement) and the other 1 fed the same diet supplemented with the commercial micronutrient (µ-nutrient supplementation) for 4 weeks before calving and 8 weeks thereafter. Milk production and composition, BW, body condition score (BCS), DM intake (DMI) and health (calving score, metritis and mastitis) were recorded over the study period. Milk samples were collected on D15 and D60 post-calving for analyses of casein, Na+ and K+ contents and metalloprotease activity. Milk leukocytes and milk mammary epithelial cells (mMECs) were purified and counted. The viability of mMECs was assessed, together with their activity, through an analysis of gene expression. At the same time points, peripheral blood mononuclear cells (PBMCs) were purified and counted. Using genomic DNA extracted from PBMCs, mMECs and milk leukocytes, we assessed global DNA methylation (Me-CCGG) to evaluate the epigenetic imprinting associated with the µ-nutrient-supplemented diet. The µ-nutrient supplementation increased BCS and BW without modifying DMI or milk yield and composition. It also improved calving condition, reducing the time interval between calving and first service. Each easily collectable cell type displayed a specific pattern of Me-CCGG with only subtle changes associated with lactation stages in PBMCs. In conclusion, the response to the µ-nutrient supplementation improved the body condition without alteration of global epigenetic status in dairy cows.

Keywords

cowsperipartummicronutrientsmethylomeperformances
Type
Research Article
Information
animal , Volume 14 , Issue 11 , November 2020 , pp. 2326 - 2335
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of The Animal Consortium

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abuelo, A, Hernández, J, Benedito, JL and Castillo, C 2015. The importance of the oxidative status of dairy cattle in the periparturient period: revisiting antioxidant supplementation. Journal of Animal Physiology and Animal Nutrition (Berl) 99, 1003–1016.Google ScholarPubMed
Agrawal, A, Khan, MJ, Graugnard, DE, Vailati-Riboni, M, Rodriguez-Zas, SL, Osorio, JS and Loor, JJ 2017. Prepartal energy intake alters blood polymorphonuclear leukocyte transcriptome during the peripartal period in Holstein cows. Bioinformatics Biology Insights 28, 1–17.Google Scholar
Allen, MS 1996. Physical constraints on voluntary intake of forages by ruminants. Journal of Animal Science 74, 3063–3075.CrossRefGoogle ScholarPubMed
Ammerman, CB and Miller, SM 1975. Selenium in ruminant nutrition: a review. Journal of Dairy Science 58, 1561–1577.Google ScholarPubMed
Andersen, CL, Jensen, JL and Ørntoft, TF 2004. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research 64, 5245–5250.CrossRefGoogle ScholarPubMed
Andrieu, S 2008. Is there a role for organic trace element supplements in transition cow health? The Veterinary Journal 176, 77–83.Google Scholar
Arreguín, A, Ribot, J, Mušinović, H, von Lintig, J, Palou, A and Bonet, ML 2018. Dietary vitamin A impacts DNA methylation patterns of adipogenesis-related genes in suckling rats. Archives of Biochemistry and Biophysics 650, 75–84.Google ScholarPubMed
Attig, L, Vigé, A, Gabory, A, Karimi, M, Beauger, A, Gross, M-S, Athias, A, Gallou-Kabani, C, Gambert, P, Ekstrom, TJ, Jais, J-P and Junien, C 2013. Dietary alleviation of maternal obesity and diabetes: increased resistance to diet-induced obesity transcriptional and epigenetic signatures. PLoS ONE 8, 1–25.Google ScholarPubMed
Boutinaud, M, Ben Chedly, MH, Delamaire, E and Guinard-Flament, J 2008. Milking and feed restriction regulate transcripts of mammary epithelial cells purified from milk. Journal of Dairy Science 91, 988–998.Google ScholarPubMed
Boutinaud, M, Herve, L and Lollivier, V 2015. Mammary epithelial cells isolated from milk are a valuable, non-invasive source of mammary transcripts. Frontiers of Genetics 6, 323–335.CrossRefGoogle ScholarPubMed
Boutinaud, M, Isaka, N, Lollivier, V, Dessauge, F, Gandemer, E, Lamberton, P, De Prado Taranilla, AI, Deflandre, A and Sordillo, LM 2016. Cabergoline inhibits prolactin secretion and accelerates involution in dairy cows after dry-off. Journal of Dairy Science 99, 5707–5718.CrossRefGoogle ScholarPubMed
Cerda, S and Weitzman, SA 1997. Influence of oxygen radical injury on DNA methylation. Mutation Research 386, 141–152.CrossRefGoogle ScholarPubMed
Chavatte-Palmer, P, Velazquez, MA, Jammes, H and Duranthon, V 2018. Review: Epigenetics, developmental programming and nutrition in herbivores. Animal 12, s363–s371.Google ScholarPubMed
Coon, RE, Duffield, TF and DeVries, TJ 2019. Short communication: Risk of subacute ruminal acidosis affects the feed sorting behavior and milk production of early lactation cows. Journal of Dairy Science 102, 652–659.CrossRefGoogle ScholarPubMed
Drackley, JK and Cardoso, FC 2014. Prepartum and postpartum nutritional management to optimize fertility in high-yielding dairy cows in confined TMR systems. Animal 8, 5–14.CrossRefGoogle ScholarPubMed
Friggens, NC, Disenhaus, C and Petit, HV 2010. Nutritional sub-fertility in the dairy cow: towards improved reproductive management through a better biological understanding. Animal 4, 1197–1213.CrossRefGoogle ScholarPubMed
Grossman, M and Koop, WJ 2003. Modeling extended lactation curves of dairy cattle: a biological basis for the multiphasic approach. Journal of Dairy Science 86, 988–998.CrossRefGoogle ScholarPubMed
Huang, D, Cui, L, Ahmed, S, Zainab, F, Wu, Q, Wang, X and Yuan, Z 2019. An overview of epigenetic agents and natural nutrition products targeting DNA methyltransferase, histone deacetylases and microRNAs. Food and Chemical Toxicology 123, 574–594.Google ScholarPubMed
Ingvartsen, KL and Moyes, K 2013. Nutrition, immune function and health of dairy cattle. Animal 7, 112–122.Google ScholarPubMed
INRA 2007. Alimentation des bovins, ovins et caprins. Edition Quae, Versailles, France.Google Scholar
LeBlanc, SJ 2012. Interactions of metabolism, inflammation, and reproductive tract health in the postpartum period in dairy cattle. Reproduction in Domestic Animals 47, 18–30.CrossRefGoogle ScholarPubMed
Lehmann, JO, Mogensen, L and Kristensen, T 2017. Early lactation production, health, and welfare characteristics of cows selected for extended lactation. Journal of Dairy Science 100, 1487–1501.CrossRefGoogle ScholarPubMed
Li, C, Batistel, F, Osorio, JS, Drackley, JK, Luchini, D and Loor, JJ 2016. Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows. Journal of Animal Science and Biotechnology 7, 18–30.Google ScholarPubMed
Lollivier, V, Lacasse, P, Angulo Arizala, J, Lamberton, P, Wiart, S, Portanguen, J, Bruckmaier, R and Boutinaud, M 2015. In vivo inhibition followed by exogenous supplementation demonstrates galactopoietic effects of prolactin on mammary tissue and milk production in dairy cows. Journal of Dairy Science 98, 8775–8787.Google ScholarPubMed
Martin, EM and Fry, RC 2018. Environmental influences on the epigenome: exposure- associated DNA methylation in human populations. Annual Review of Public Health 39, 309–333.Google ScholarPubMed
Mehdi, Y and Dufrasne, I 2016. Selenium in cattle: a review. Molecules 21, 545–559.Google ScholarPubMed
Mentch, SJ and Locasale, JW 2016. One-carbon metabolism and epigenetics: understanding the specificity. Annals of the New York Academy of Sciences 1363, 91–98.CrossRefGoogle ScholarPubMed
Nguyen, M, Boutinaud, M, Pétridou, B, Chat, S, Bouet, S, Laloë, D, Jaffrezic, F, Gabory, A, Kress, C, Galio, L, Charlier, M, Pannetier, M, Klopp, C, Jammes, H and Devinoy, E 2013. La monotraite induit la méthylation d’une région régulatrice distale en amont du gène de la caséine-S1. In: 20èmes Rencontres Recherches Ruminants. Paris, France (4-5 décembre, 2013), Institut de l’Elevage – INRA, 79–81.Google Scholar
Perrier, JP, Sellem, E, Prézelin, A, Gasselin, M, Jouneau, L, Piumi, F, Al Adhami, H, Weber, M, Fritz, S, Boichard, D, Le Danvic, C, Schibler, L, Jammes, H and Kiefer, H 2018. A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific features. BioMed Central (BMC) Genomics 19, 404–422.Google ScholarPubMed
Piepers, S and De Vliegher, S 2013. Oral supplementation of medium-chain fatty acids during the dry period supports the neutrophil viability of peripartum dairy cows. Journal of Dairy Research 80, 309–318.Google ScholarPubMed
Politis, I 2012. Reevaluation of vitamin E supplementation of dairy cows: bioavailability, animal health and milk quality. Animal 6, 1427–1434.Google ScholarPubMed
Rabot, A, Sinowatz, F, Berisha, B, Meyer, HH, Schams, D 2007. Expression and localization of extracellular matrix-degrading proteinases and their inhibitors in the bovine mammary gland during development, function, and involution. Journal of Dairy Science 90, 740–748.CrossRefGoogle ScholarPubMed
Ragaller, V, Hüther, L and Lebzien, P 2009. Folic acid in ruminant nutrition: a review. British Journal of Nutrition 10, 153–164.Google Scholar
Rainard, P and Riollet, C 2006. Innate immunity of the bovine mammary gland. Veterinary Research 37, 369–400.CrossRefGoogle ScholarPubMed
Reinius, LE, Acevedo, N, Joerink, M, Pershagen, G, Dahlén, SE, Greco, D, Söderhäll, C, Scheynius, A and Kere, J 2012. Differential DNA methylation in purified human blood cells: implications for cell lineage and studies on disease susceptibility. PLoS One 7, 1–13.Google ScholarPubMed
SAS Institute 1999. SAT/STAT user’s guide: statistics, version 8, 1st edition. SAS Institute Inc., Cary, NC, USA.Google Scholar
Sordillo, LM 2016. Nutritional strategies to optimize dairy cattle immunity. Journal of Dairy Science 99, 4967–4982.CrossRefGoogle ScholarPubMed
Sordillo, LM and Aitken, SL 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology 128, 104–109.CrossRefGoogle ScholarPubMed
Spears, JW and Weiss, WP 2008. Role of antioxidants and trace elements in health and immunity of transition dairy cows. The Veterinary Journal 176, 70–76.Google ScholarPubMed
Speckmann, B and Grune, T 2015. Epigenetic effects of selenium and their implication for health. Epigenetics 10, 179–190.Google Scholar
Yart, L, Lollivier, V, Finot, L, Dupont, J, Wiart, S, Boutinaud, M, Marnet, PG and Dessauge, F 2013. Changes in mammary secretory tissue during lactation in ovariectomized dairy cows. Steroids 78, 973–981.CrossRefGoogle ScholarPubMed
Zebeli, Q, Ghareeb, K, Humer, E, Metzler-Zebeli, BU and Besenfelder, U 2015. Nutrition, rumen health and inflammation in the transition period and their role on overall health and fertility in dairy cows. Research in Veterinary Science 103, 126–136.CrossRefGoogle ScholarPubMed
Zecconi, A, Albonico, F, Gelain, ME, Piccinini, R, Cipolla, M and Mortarino, M 2018. Effects of herd and physiological status on variation of 16 immunological and inflammatory parameters in dairy cows during drying off and the transition period. Journal of Dairy Research 85, 167–173.CrossRefGoogle ScholarPubMed
Supplementary material: File

Gasselin et al. supplementary material

Gasselin et al. supplementary material

Download Gasselin et al. supplementary material(File)
File 101.1 KB