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Effects of the Texel muscling quantitative trait locus on carcass traits in crossbred lambs

Published online by Cambridge University Press:  19 November 2008

J. M. Macfarlane ,
N. R. Lambe ,
S. C. Bishop ,
O. Matika ,
E. Rius-Vilarrasa ,
K. A. McLean ,
W. Haresign ,
B. T. Wolf ,
R. J. McLaren  and
L. Bünger
J. M. Macfarlane*
Affiliation:
Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
N. R. Lambe
Affiliation:
Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
S. C. Bishop
Affiliation:
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9PS, UK
O. Matika
Affiliation:
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9PS, UK
E. Rius-Vilarrasa
Affiliation:
Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
K. A. McLean
Affiliation:
Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
W. Haresign
Affiliation:
Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Llanbadarn Campus, Aberystwyth SY23 3AL, UK
B. T. Wolf
Affiliation:
Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Llanbadarn Campus, Aberystwyth SY23 3AL, UK
R. J. McLaren
Affiliation:
AgResearch MBU, Biochemistry Department, University of Otago, Dunedin, New Zealand
L. Bünger
Affiliation:
Sustainable Livestock Systems Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG, UK
*
E-mail: [email protected]
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Abstract

Texel muscling quantitative trait locus (TM-QTL) is a QTL on chromosome 18, originally identified in purebred UK Texel sheep, which was reported to increase ultrasonically measured muscle depth at the third lumbar vertebra by around 4% to 7%. The objective of the present study was to comprehensively evaluate the TM-QTL and to determine whether it could provide benefits to the UK sheep industry through increased carcass meat yield in crossbred slaughter lambs. Effects of this QTL on a range of carcass traits, including those measured in vivo and by dissection, were evaluated in heterozygous carrier and non-carrier lambs produced by crossing heterozygous carrier Texel rams with non-carrier Mule (Bluefaced Leicester × Scottish Blackface) ewes from a lowland flock. The TM-QTL was found to increase loin muscling in crossbred lambs at a given live weight or carcass weight, as measured by ultrasound, X-ray computed tomography (CT) and carcass dissection. Depth of M. longissimus lumborum (MLL) was greater in TM-QTL carrier lambs compared to non-carriers as measured by both ultrasound at the third lumbar vertebra (+4.5%; P = 0.033) and CT scanning at the fifth lumbar vertebra (+6.7%; P = 0.004). Width and area of MLL measured using CT were also greater in TM-QTL carrier lambs compared to non-carriers (+3.0%; P = 0.013 and +5.1%; P = 0.047, respectively). Loin muscle volume measured using CT was greater in TM-QTL carriers than in non-carriers (+5.9%; P = 0.005) and the dissected weight of the MLL was +7.1% greater in TM-QTL carriers compared to non-carriers (P < 0.001). The proportion of the total carcass lean meat yield (LMY) that was contained within the loin region was slightly higher in TM-QTL carriers than in non-carriers (0.154 v. 0.145; P = 0.006). However, TM-QTL was found to have no significant effect on the total weight or proportion of LMY or of saleable meat yield in the carcass measured by dissection, or on muscling in the hind leg measured by CT or dissection. This work has verified that the inheritance of TM-QTL is associated with increased loin muscling in crossbred lambs, as has previously been reported for purebred Texel lambs.

Keywords

carcassmusclingsheepTexelQTL
Type
Full Paper
Information
animal , Volume 3 , Issue 2 , February 2009 , pp. 189 - 199
Copyright
Copyright © The Animal Consortium 2008

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References

Arnold, HH, Della-Fera, MA, Baile, CA 2001. Review of myostatin history, physiology and applications. International Archives of Bioscience 2001, 1014–1022.Google Scholar
Arthur, PF 1995. Double muscling in cattle: a review. Australian Journal of Agricultural Research 46, 1493–1515.CrossRefGoogle Scholar
Bain, WE, Johnson, PL, Greer, GJ, Dodds, KG, McLean, NJ, McLaren, RJ, Galloway, SM, van Stijn, TC, McEwan, JC 2008. The effect of MyoMAX® on carcass lean and fat. Proceedings of the New Zealand Society of Animal Production 68, 43–44.Google Scholar
Banks, R 1997. The Meat Elite Project: establishment and achievements of an elite meat sheep nucleus. Proceedings of the Association for the Advancement of Animal Breeding and Genetics 12, 598–601.Google Scholar
Broad, TE, Glass, BC, Greer, GJ, Robertson, TM, Bain, WE, Lord, EA, McEwan, JC, Peterson, SW 2000. Search for a locus near to myostatin that increases muscling in Texel sheep in New Zealand. Proceedings of the New Zealand Society of Animal Production 60, 110–112.Google Scholar
Bünger, L, Ott, G, Varga, L, Schlote, W, Rehfeldt, C, Renne, U, Williams, JL, Hill, WG 2004. Marker assisted introgression of the Compact mutant myostatin allele: Mstn(^Cmpt-dl1Abc) into a mouse line with extreme growth-effects on body composition and muscularity. Genetical Research 84, 161–173.CrossRefGoogle Scholar
Campbell, AW, McLaren, RJ 2007. LoinMAXTM and MyoMAX™: taking DNA marker tests from the research environment to commercial reality. Proceedings of New Zealand Society of Animal Production 67, 160–162.Google Scholar
Clop, A, Marcq, F, Takeda, H, Pirottin, D, Tordoir, X, Bibe, B, Bouix, J, Caiment, F, Elsen, JM, Eychenne, F, Larzul, C, Laville, E, Meish, F, Milenkovic, D, Tobin, J, Charlier, C, Georges, M 2006. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nature Genetics 38, 813–818.CrossRefGoogle ScholarPubMed
Duckett, SK, Snowder, GD, Cockett, NE 2000. Effect of the callipyge gene on muscle growth, calpastatin activity, and tenderness of three muscles across the growth curve. Journal of Animal Science 78, 2836–2841.CrossRefGoogle ScholarPubMed
Fernando, FL, Grossman, M 1989. Marker assisted selection using best linear unbiased prediction. Genetics Selection Evolution 21, 467–477.CrossRefGoogle Scholar
Freking, BA, Murphy, SK, Wylie, AA, Rhodes, SJ, Keele, JW, Leymaster, KA, Jirtle, RL, Smith, TP 2002. Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals. Genome Research 12, 1496–1506.CrossRefGoogle ScholarPubMed
GenStat 9 Committee 2006. GenStat. Lawes Agricultural Trust, Rothamstead Experimental Station, Harpenden, UK.Google Scholar
Georges, M, Cockett, N 1996. The ovine callipyge locus: a paradigm illustrating the importance of non-Mendelian genetics in livestock. Reproduction Nutrition Development 36, 651–657.Google ScholarPubMed
Jackson, SP, Miller, MF, Green, RD 1997a. Phenotypic characterization of Rambouillet sheep expressing the callipyge gene: II. Carcass characteristics and retail yield. Journal of Animal Science 75, 125–132.CrossRefGoogle ScholarPubMed
Jackson, SP, Miller, MF, Green, RD 1997b. Phenotypic characterization of Rambouillet sheep expressing the callipyge gene: III. Muscle weights and muscle weight distribution. Journal of Animal Science 75, 133–138.CrossRefGoogle ScholarPubMed
Johnson, PL, McEwan, JC, Dodds, KG, Purchas, RW, Blair, HT 2005. A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in Texel sheep. Journal of Animal Science 83, 1988–2000.CrossRefGoogle ScholarPubMed
Jones, HE, Lewis, RM, Young, MJ, Wolf, BT 2002. The use of X-ray computer tomography for measuring the muscularity of live sheep. Animal Science 75, 387–399.CrossRefGoogle Scholar
Jopson, NB, Nicoll, GB, Stevenson-Barry, JM, Duncan, S, Greer, GJ, Bain, WE, Gerard, EM, Glass, BC, Broad, TE, McEwan, JC 2001. Mode of inheritance and effects on meat quality of the rib-eye muscling (REM) QTL in Sheep. Proceedings of the Association for the Advancement of Animal Breeding and Genetics 14, 111–114.Google Scholar
Kijas, JW, McCulloch, R, Edwards, JE, Oddy, VH, Lee, SH, van der Werf, J 2007. Evidence of multiple alleles effecting muscling and fatness at the ovine GDF8 locus. BMC Genetics 8, 80.CrossRefGoogle ScholarPubMed
Koohmaraie, M, Shackelford, SD, Wheeler, TL, Lonergan, SM, Doumit, ME 1995. A muscle hypertrophy condition in lamb (callipyge): characterization of effects on muscle growth and meat quality traits. Journal of Animal Science 73, 3596–3607.CrossRefGoogle ScholarPubMed
Laville, E, Bouix, J, Sayd, T, Bine, B, Elsen, JM, Larzul, C, Eychenne, F, Marcq, F, Georges, M 2004. Effects of a quantitative trait locus for muscle hypertrophy from Belgian Texel sheep on carcass conformation and muscularity. Journal of Animal Science 82, 3128–3137.CrossRefGoogle ScholarPubMed
Mann AD, Young MJ, Glasbey CA and McLean KA 2003. STAR: Sheep Tomogram Analysis Routines (V.3.4). BioSS Software Documentation.Google Scholar
Marcq F, Larzul C, Marot V, Bouix J, Eychenne F, Laville E, Bibe B, Le Roy PL, Georges M and Elsen J-M 2002. Preliminary results of a whole genome scan targeting QTL for carcass traits in a Texel × Romanov intercross. Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France, comm. no. 02-14.Google Scholar
Matika O, Pong-Wong R, Woolliams JA, Low J, Nieuwhof GJ, Boon S and Bishop SC 2006. Verifying quantitative trait loci for muscle depth in commercial terminal sire sheep. Proceedings of the 8th World Congress on Genetics Applied to Livestock Production, Belo Horizonte, Brazil, comm. no. 22-10.Google Scholar
Navajas, EA, Glasbey, CA, McLean, KA, Fisher, AV, Charteris, AJL, Lambe, NR, Bunger, L 2006. In vivo measurements of muscle volume by automatic image analysis of spiral computed tomography scans. Animal Science 82, 545–553.CrossRefGoogle Scholar
Navajas, EA, Lambe, NR, McLean, KA, Glasbey, CA, Fisher, AV, Charteris, AJL, Bunger, L, Simm, G 2007. Accuracy of in vivo muscularity indices measured by computed tomography and their association with carcass quality in lambs. Meat Science 75, 533–542.CrossRefGoogle ScholarPubMed
Nicoll, GB, Burkin, HR, Broad, TE, Jopson, NB, Greer, GJ, Bain, WE, Wright, CS, Dodds, KG, Fennessy, PF, McEwan, JC 1998. Genetic linkage of microsatellite markers to the Carwell locus for rib-eye muscling in sheep. Proceedings of the 6th World Congress on Genetics applied to Livestock Production, Armidale, Australia 26, 529–532.Google Scholar
Pollott GE and Stone DG 2006. Breeding rams. In The breeding structure of the British sheep industry 2003 (ed. RD Eglin, A Ortiz Pelaez and CJ Cook). Defra, London (http://www.defra.gov.uk/animalh/bse/othertses/scrapie/nsp/publicatsrpts/pollott2003.pdf).Google Scholar
Pong-Wong, R, George, AW, Woolliams, JA, Haley, CS 2001. A simple and rapid method for calculating identity-by-descent matrices using multiple markers. Genetics Selection Evolution 33, 453–471.CrossRefGoogle ScholarPubMed
Seaton, G, Hayley, CS, Knott, SA, Kearsey, M, Visscher, PM 2002. QTL Express: mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics 18, 339–340.CrossRefGoogle ScholarPubMed
Simm, G, Dingwall, WS 1989. Selection indices for lean meat production in sheep. Livestock Production Science 21, 223–233.CrossRefGoogle Scholar
Smit, M, Segers, K, Carrascosa, LG, Shay, T, Baraldi, F, Gyapay, G, Snowder, G, Georges, M, Cockett, N, Charlier, C 2003. Mosaicism of solid gold supports the causality of a non-coding A-to-G transition in the determinism of the Callipyge phenotype. Genetics 163, 453–456.CrossRefGoogle Scholar
Walling, GA, Visscher, PM, Wilson, AD, McTeir, BL, Simm, G, Bishop, SC 2004. Mapping of quantitative trait loci for growth and carcass traits in commercial sheep populations. Journal of Animal Science 82, 2234–2245.CrossRefGoogle ScholarPubMed