Captive bolts or firearms are unsuitable for euthanasia of livestock when an intact brain is required for diagnostics. Injectable barbiturates can be used, but this method carries risk of poisoning animals eating the carcase. Intravenous saturated salt solutions have been used to euthanase heavily sedated ruminants and are cheap, readily available and not a risk to scavenging animals. However, there is concern that they may be painful or cause distress to animals that are not unconscious. This study aimed to determine the suitability of saturated salt solutions, in combination with xylazine, as a method of euthanasia of ruminants using a sheep model. Thirty-two sheep were sedated with xylazine (0.4 mg kg-1 IM) and euthanased with an intravenous overdose of pentobarbitone (PENT; n = 10), saturated potassium chloride (KCL; n = 11) or saturated magnesium sulphate (MGS; n = 10). Time until end of rhythmic breathing and cardiac arrest, and movement events were recorded. Conscious perception of pain was evaluated by measuring cortical brain activity by electroencephalography (EEG). There was no evidence of perceived pain or unpleasant sensory experience for any treatment as indicated by P50, P95 and Ptot and so all methods were deemed humane. Time until transient EEG was comparable for all treatments. Time until onset of isoelectric EEG was prolonged for KCL. Animals euthanased with KCL consistently exhibited severe reflex movements during infusion (eg kicking, convulsion). No severe movement events were observed in animals euthanased with MGS, hence, physiological and movement data support the preferential use of MGS over KCL.

Reporting of outcome variables by caregivers in welfare studies is commonplace but is open to subjective bias and so requires validation. Biases can occur in either direction: familiarity with an animal allows a deeper insight into welfare problems, but also can lead to reticence in admitting that an animal in one's care is experiencing problems. Here, we aim to validate owner-reporting of plumage condition of pet parrots, including those with self-inflicted feather-damaging behaviour (FDB), by comparing owners’ scores of feather condition with those of two independent raters, blind to the owners’ and each other's assessments. We surveyed pet parrot owners to collect data on basic demographics and feather condition, and requested four standardised photographs of birds. We received 259 responses (17% of the 1,521 people contacted); 78 sets of images of appropriate quality for assessment by raters were provided. Mean percentage agreement between owners’ and raters’ scores was mostly fair to substantial using Cohen's kappa; however, raters scored a greater proportion of feather damage than did owners. Overall, our results indicate owner-reporting of feather condition, including FDB, to be generally reliable and consistent with independent assessment of photographs. As the use of photographs can be limited by image quality, a failure to represent the long-term state of a parrot, and the potential for incorrect recording if assessed without relevant information (eg on moulting), this evidence that owner-reports can be reliable opens the door for larger-scale surveys of the extent of welfare-relevant problems.

The adoption of slaughter and pre-slaughter techniques to improve animal welfare and meat quality and safety is dependent on providing appropriate information, application and enforcement as well as information ownership by local people. The World Society for the Protection of Animals (WSPA) and Beijing Chaoyang Anhua Animal Product Safety Research Institute (APSRI) have been running a humane slaughter programme in China for more than three years. The aim of the programme is to improve the welfare of animals slaughtered in China through a three-pronged approach: i) train core-plant mid-level managers to be humane slaughter trainers; ii) provide guidance on legislation and codes of practice; and iii) develop and implement pre-slaughter and slaughter animal welfare curricula for undergraduate veterinarians. The production of in-country training material is based on a training-needs analysis conducted prior to the programme. Four Chinese trainers have completed the training and become national level trainers. In the last three years they have trained more than 5,000 staff from 800 slaughterhouses. Technical standards for slaughtering pigs, based on those of the OIE, have been adopted in China and the veterinary curriculum development is progressing. The programme has introduced many positive changes to the Chinese slaughter industry and the signs are positive for good progress.

Capture-mark-recapture (CMR) repeated at intervals is a commonly used method of population estimation of mammals. Most guidelines for the use of wild mammals recommend adequate measures to ensure a captured animal has food and water available, as needed, until it is released. Small mammals are often highlighted as needing special care, but recommendations are either less clear or absent for medium- and large-sized mammals. We therefore examined the short-term welfare implications of CMR trapping of medium-sized mammals, using the marsupial, brushtail possum (Trichosurus vulpecula) as a case study. Possums were caught in cage traps baited with circa 30 g of food and set for three consecutive days at monthly intervals. Timing devices on the traps measured when traps were sprung. Possums were confined in traps for, on average, 89% of each night they were trapped, plus a further 2-6 h between sunrise and the actual time of release from the traps. They lost 3-8% of their bodyweight on average, depending on the number of captures in a trapping session. A recently developed framework for humaneness assessment, based on five welfare domains, gave an overall welfare impact of mild-moderate for cage trapping of possums. The short-term solutions to these welfare impacts would be, at a minimum, to provide trapped possums with adequate additional food and water, and minimise their time spent in the traps. Guidelines for the use of wild animals in research and management could also be more prescriptive regarding the provision of food and water in studies of medium-sized mammals that involve repeated capture.

Veterinarians and animal scientists can provide leadership on issues relevant to farm animal welfare, but perceptions of these stakeholders regarding societal expectations for welfare are underexplored. This study involved five focus groups of veterinarians and animal scientists (n = 50 in total), recruited at a European meeting focused on cattle welfare. Participants were invited to discuss topics related to cattle welfare and were prompted with questions to elicit their perspectives of public concerns and how the participants felt public input should be included when developing solutions. Discussions were moderated by trained facilitators, audio-recorded and transcribed, and transcripts analysed using reflexive thematic analysis. Ultimately, four primary themes were developed: (1) The public as concerned; (2) The public as ignorant; (3) The public as needing education; and (4) The public as helper or hindrance. Groups identified specific farming practices viewed as concerning to the public, including lack of pasture access, behavioural restriction, and painful procedures. Discussions about these concerns and the role of the public were often framed around the assumption that the public was ignorant about farming, and that this ignorance needed to be rectified through education. Participants were generally ambivalent in their beliefs regarding public contributions to solutions for farm animal welfare but suggested that consumers should pay more for products to help shoulder any costs of welfare improvements.

Animal protection laws exist at federal, provincial and municipal levels in Canada, with enforcement agencies relying largely upon citizens to report concerns. Existing research about animal protection law focuses on general approaches to enforcement and how legal terms function in the courts, but the actual work processes of animal law enforcement have received little study. We used institutional ethnography to explore the everyday work of Call Centre operators and Animal Protection Officers, and we map how this work is organised by laws and institutional polices. When receiving and responding to calls staff try to identify evidence of animal ‘distress’ as legally defined, because various interventions (writing orders, seizing animals) then become possible. However, many cases, such as animals living in deprived or isolated situations, fall short of constituting ‘distress’ and the legally mandated interventions cannot be used. Officers are also constrained by privacy and property law and by the need to record attempts to secure compliance in order to justify further action including obtaining search warrants. As a result, beneficial intervention can be delayed or prevented. Officers sometimes work strategically to advocate for animals when the available legal tools cannot resolve problems. Recommendations arising from this research include expanding the legal definition of ‘distress’ to better fit animals’ needs, developing ways for officers to intervene in a broader range of situations, and more ethnographic research on enforcement work in jurisdictions with different legal systems to better understand how animal protection work is organised and constrained by laws and policies.

The objective of this study was to evaluate the use of flavoured ropes as environmental enrichment for individually housed pigs (Sus scrofa). A 4 × 4 Latin square experimental design was utilised to evaluate the effects of four rope flavour treatments on 24 individually penned gilts: i) water, ii) salt water solution; iii) sugar water solution; and iv) apple juice. Cotton rope was soaked in the assigned treatment solution for 30 min on day 1 of each treatment. The rope was tied to an overhead bar at 1000h on day 1 and removed at 1900h on day 2. The following day, gilts received a different treatment using the same methodology. Gilts were videorecorded one day before treatments were given (baseline) and throughout the study. The video was analysed for enrichment interaction, eating behaviour, and posture using a 2-min scan sample interval between 0700 and 1900h. The addition of sugar flavour increased enrichment interaction compared to apple and salt flavours but did not differ compared to water treatment. Furthermore, gilts interacted with enrichment 61% more the first day the enrichment flavour treatment was provided compared to the second. Gilts given rope enrichment spent less time lying and more time sitting compared to when no enrichment was provided. The results of this study suggest that while the addition of flavours to cotton ropes caused minor changes in enrichment interaction and behaviour, provision of rope enrichment was beneficial for increasing activity in stalled gilts.

Many captive Asian elephants (Elephas maximus) in Thailand participate in the tourism industry at attractions known as ‘elephant camps.’ There has been significant criticism of low welfare venues, where the elephants may experience injuries, poor nutrition, unnatural social environments and aversive handling. Despite increasing concern for animal welfare, the general public often have difficulty identifying the welfare issues affecting captive animals. The aim of this study was to investigate participants’ willingness to support an elephant attraction and their perceived emotional value from the experience, based on the affective state of the captive elephant and their level of contact with it. Participants (n = 590) from the United States were randomly assigned to one of four vignettes (using a 2 × 2 experimental design) that described an elephant attraction, varying the affective state of the elephant (feels excellent, feels terrible) and the level of contact they could have with the elephant (low, high). A mixed methods approach was used, where participants provided answers to Likert-type questions, followed by an open-ended response. Participants showed greater willingness to support the elephant attraction and greater perceived emotional value from the experience when the elephant felt excellent, as opposed to when the elephant felt terrible. There were no significant differences between low and high contact for the measures included in this study. Qualitative responses varied greatly, with participants making many assumptions about the elephant and the attraction, revealing potential misconceptions that they had regarding the welfare of captive elephants. This research may be used to encourage a shift in tourism preferences to venues that reflect positive elephant welfare.

Multiple benefits of freight rail activity have been shown for commercial agribusiness, yet the effects of freight rail-related noise and vibration on domestic livestock health and welfare has so far received little research attention. This scoping review examines peer-reviewed and grey literature addressing associations between freight rail noise, vibration and impacts on domestic livestock. Six databases (Scopus, Science Direct, SAGE, TRID, SPARK, ARRB) were searched for relevant literature published from 1980–2019. PRISMA search procedures were used to identify 28 publications relevant to domestic livestock, as well as noise or vibration impact of rail applicable to the freight rail context. Included publications addressed a range of livestock and related species, covering descriptive, review, and experimental findings on noise and vibration impacts. Five publications addressed vibration effects, and 23 addressed noise effects. Effects of noise and vibration on different species indicated that adverse effects vary depending on exposure intensity. The literature indicates that specific thresholds for noise and vibration exposure should be considered when managing freight rail impacts on commercial agribusiness involving avian and mammalian species. Freight rail noise and vibration likely exceeds thresholds for discomfort and harm for avian and mammalian species. Future research should consider case studies that specifically focus on integrating freight rail noise and vibration data to derive species-specific guides for animal health and welfare purposes.

Monitoring the concentration of glucocorticoid metabolites (GCMs) in faecal samples is a non-invasive tool for physiological stress evaluation, particularly useful when studying wild species. However, both negative and positive stimuli (distress and eustress, respectively) can lead to a rise in glucocorticoids. Thus, besides validating whether GCM concentration in faeces reflects endogenous adrenal activity, we also need to identify behavioural indicators of distress to avoid misinterpretation. Therefore, we submitted four adult male spotted pacas (Cuniculus paca) to an exogenous adrenocorticotropic hormone (ACTH) challenge-test in a Latin square design (4 × 4) to monitor changes in the GCM concentration in faeces. We also aimed to describe behaviours potentially indicative of distress. We collected excreted faeces and video-recorded the animals’ behaviours for five consecutive days, one day before and four days after application of the following four treatments: 1st control (no-handling); 2nd control (intra-muscular [IM] injection of saline solution); low-dose ACTH (IM injection of 0.18 ml ACTH); and high-dose ACTH (IM injection of 0.37 ml ACTH). There was a peak in the concentration of GCM in faeces collected 24 h after the injection of the high-dose ACTH treatment. Additionally, independent of the treatments, spotted pacas spent less time on exploration and feeding states, while spending more time in the inactive but awake (IBA) state following the treatment application (challenge day). The use of GCM concentration in faecal samples together with the behavioural changes (less exploration and feeding, and more IBA) showed to be efficient as a non-invasive tool for welfare assessment of farmed spotted paca.