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Follicle populations, ovulation rates and profiles of gonadotropins, inhibin and insulin-like growth factor-1 of heifers actively immunized against androstenedione and inhibin, separately or in combination

Published online by Cambridge University Press:  02 September 2010

S. M. Rhind ,
S. R. Schemm  and
B. D. Schanbacher
S. M. Rhind
Affiliation:
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB9 2QJ
S. R. Schemm
Affiliation:
US Department of Agriculture, Agriculture Research Service, Roman L. Hruska US Meat Animal Research Center, Clay Center, Nebraska 68933–0166, USA
B. D. Schanbacher
Affiliation:
US Department of Agriculture, Agriculture Research Service, Roman L. Hruska US Meat Animal Research Center, Clay Center, Nebraska 68933–0166, USA
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Abstract

Four groups of 20 Simmental cross heifers were actively immunized against human serum albumin (control, (C), androstenedione (A), a 30 amino acid synthetic fragment of porcine inhibin alpha (I) or A and I in combination (AI). At the time of the third and final antigen injection and again 2 weeks later, all animals were injected with synthetic prostaglandin F2α (PG) to synchronize oestrus. Concentrations of LH, FSH, insulin-like growth factor-1 (IGF-1) and inhibin (C and A heifers only) in serum samples collected twice daily during 3 days before the second PG injection and at 15-min intervals from 24 to 32 h after PG injection were determined. Two weeks later, all heifers were administered PG and ovariectomized 24 h later. Ovarian follicles were dissected from the stroma, measured and cultured in medium 199 for 2h at 37°C. Numbers of small (3·0 to 7·9 mm diameter) and large (>8·0 mm diameter) follicles were not significantly altered by any of the immunization treatments. Intrafollicular concentrations and secretion rates of oestradiol, testosterone, progesterone and inhibin, determined in vitro, were not affected by immunization. Intrafollicular IGF-1 concentrations were not affected; this hormone was not detectable in the culture medium. Intrafollicular concentrations of oestrogen and testosterone and secretion rates of all three steroids were greater (P < 0·05) in animals which had regressing corpora lutea at the time of ovariectomy. Of the animals that had dominant follicles, one of 17, one of 13, four of 19 and six of 15 in the C, A, I and AI groups, respectively, had two or more. Only the difference between AI and C cows in the incidence of dominant follicles was statistically significant (P < 0·05). Mean circulating concentrations of LH, and inhibin (C and A groups only) and mean LH pulse frequency and pulse amplitude were unaffected by treatment but FSH concentrations were higher in A (P < 0·001) and lower in AI and I heifers (P < 0·001) compared with C animals while IGF-1 concentrations were lower in A heifers (P < 0·001) and higher in AI heifers (P < 0·05) compared with C heifers. It is concluded that immunization against inhibin, either alone or in combination with immunization against androstenedione increased the incidence of multiple dominant follicles in heifers but did not increase their ovulation rate.

Keywords

folliclesheifershormonesimmunization
Type
Research Article
Information
Animal Production , Volume 57 , Issue 1 , August 1993 , pp. 55 - 63
Copyright
Copyright © British Society of Animal Science 1993

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References

Adashi, E. Y., Resnick, C. E., D'Ercole, A. J., Svoboda, M. E. and Van Wyk, J. J. 1985. Insulin-like growth factors as intraovarian regulators of granulosa cell growth and function. Endocrine Reviews 6:400–420.CrossRefGoogle Scholar
Baird, D. T., Swanston, I. A. and McNeilly, A. S. 1981. Relationships between LH, FSH and prolactin concentration and the secretion of androgens and estrogens by the preovulatory follicle in the ewe. Biology of Reproduction 24: 1013–1025.Google Scholar
Carson, R. S., Zhang, Z., Hutchinson, L. A., Herrington, A. and Findlay, J. K. 1989. Growth factors in ovarian function. Journal of Reproduction and Fertility 85: 735–746.Google Scholar
Echternkamp, S. E., Spicer, L. J., Gregory, K. E., Canning, S. F. and Hammond, J. M. 1990. Concentrations of insulin-like growth factor-I in blood and ovarian follicular fluid of cattle selected for twins. Biology of Reproduction 43: 8–14.Google Scholar
Kesler, D. J., Garverick, H. A., Youngquist, R. S., Elmore, R. G. and Bierschival, C. J. 1977. Effects of days postpartum and endogenous reproductive hormones on GnRH-induced LH release in dairy cows, journal of Animal Science 45: 797–803.Google Scholar
Land, R. B., Morris, B. A., Baxter, G., Fordyce, M. and Forster, J. 1982. Improvement of sheep fecundity by treatment with antisera to gonadal steroids, journal of Reproduction and Fertility 66: 625–634.Google Scholar
McNeilly, A. S., Jonassen, J. A. and Rhind, S. M. 1987. Reduced ovarian follicular development as a consequence of low body condition in ewes. Ada Endocrinologica 115: 75–83.Google ScholarPubMed
McNeilly, A. S., Picton, H. M., Campbell, B. K. and Baird, D. T. 1991. Gonadotrophic control of follicle growth in the ewe. journal of Reproduction and Fertility 43: suppl., pp. 177–186.Google Scholar
Martensz, N. D. and Scaramuzzi, R. J. 1979. Plasma concentrations of luteinizing hormone, follicle-stimulating hormone and progesterone during the breeding season in ewes immunized against androstenedione or testosterone. journal of Endocrinology 81:249–259.Google Scholar
Martin, G. B., Price, C. A., Thiery, J. C. and Webb, R. 1988. Interaction between inhibin, oestradiol and progesterone in the control of gonadotrophin secretion in the ewe. Journal of Reproduction and Fertility 82:319–328.Google Scholar
Maurer, R. and Echternkamp, S. E. 1982. Hormonal asynchrony and embryonic development. Theriogenology 17: 11–22.Google Scholar
Pathiraja, N., Carr, W. R., Fordyce, M., Forster, P., Land, R. B. and Morris, B. A. 1984. Concentration of gonadotrophins in the plasma of sheep given gonadal steroid antisera to raise ovulation rate. Journal of Reproduction and Fertility 72: 93–100.Google Scholar
Prado, R., Rhind, S. M., Wright, I. A., Russel, A. J. F., McMillen, S. R., Smith, A. J. and McNeilly, A. S. 1990. Ovarian follicle populations, steroidogenicity and micromorphology at 5 and 9 weeks post partum in beef cows in two levels of body condition. Animal Production 51: 103–108.Google Scholar
Price, C. A., Morris, B. A., O'Shea, T. and Webb, R. 1987a. Active immunization of cattle against partly purified follicular fluid from sheep. Journal of Reproduction and Fertility 81:161–168.Google Scholar
Price, C. A., Morris, B. A. and Webb, R. 1987b. Reproductive and endocrine effects of active immunization against a testosterone conjugate in the heifer. Journal of Reproduction and Fertility 81: 149–160.Google Scholar
Rhind, S. M., Leslie, I. D., Gunn, R. G. and Doney, J. M. 1985. Plasma FSH, LH, prolactin and progesterone profiles of Cheviot ewes with different levels of intake before and after mating and associated effects on reproductive performance. Animal Reproduction Science 8: 301–313.Google Scholar
Rhind, S. M. and McNeilly, A. S. 1986. Follicle populations, ovulation rates, plasma profiles of LH, FSH and prolactin in Scottish Blackface ewes in high and low levels of body condition. Animal Reproduction Science 10: 105–115.Google Scholar
Schanbacher, B. D. 1988. Radioimmunoassay of inhibin: serum responses to unilateral and bilateral orchidectomy. Endocrinology 123: 2323–2330.Google Scholar
Schanbacher, B. D. 1989. Ovulation response and pituitary FSH charge microheterogeneity in beef heifers vaccinated against synthetic porcine inhibin alpha, journal of Animal Science 67: suppl. 1, p. 361.Google Scholar
Schanbacher, B. D. and D'Occhio, M. J. 1982. Validation of direct radioimmunoassay for testosterone in unextracted serum from five species: application to study of the hypothalamic-pituitary-gonadal axis in males, journal of Andrology 3: 45–51.Google Scholar
Schanbacher, B. D. and Ford, J. J. 1976. Seasonal profiles of plasma luteinizing hormone, testosterone and estrogen in the ram. Endocrinology 99: 752–757.Google Scholar
Schanbacher, B. D. and Ford, J. J. 1977. Gonadotropin secretion in cryptorchid and castrate rams and the acute effects of exogenous steroid treatment. Endocrinology 100: 387–393.Google Scholar
Spicer, L. J., Matton, P., Echternkamp, S. E., Convey, E. M. and Tucker, H. A. 1987. Relationships between histological signs of atresia, steroids in follicular fluid and gonadotropin binding in individual bovine antral follicles during post partum anovulation. Biology of Reproduction 36: 890–898.Google Scholar
Statistical Analysis Systems Institute. 1985. SAS user's guide: statistics. Version 5. SAS Institute Inc., Cary, NC.Google Scholar
Trout, W. E. and Schanbacher, B. D. 1990. Growth hormone and insulin-like growth factor-1 responses in steers actively immunized against somatostatin or growth hormone-releasing factor, journal of Endocrinology 125: 123–129.Google Scholar
Van Kessel, A. G., Korchinski, R. S., Hampton, C. H. and Laarveld, B. 1990. Effects of immunization against somatostatin in the pregnant ewe on growth and endocrine status of the neonatal lamb. Domestic Animal Endocrinology 7:217–227.Google Scholar
Wise, T. and Schanbacher, B. D. 1983. Reproductive effect of immunizing heifers against androstenedione and oestradiol-17β. journal of Reproduction and Fertility 69: 605–612.Google Scholar
Wrathall, J. H. M., McLeod, B. J., Glencross, R. G., Beard, A. J. and Knight, P. G. 1990. Inhibin immunoneutralization by antibodies raised against synthetic peptide sequences of inhibin subunit: effects on gonadotrophin concentrations and ovulation rate in sheep. Journal of Endocrinology 124: 167–176.Google Scholar