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The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender

Published online by Cambridge University Press:  02 September 2010

C. Alexopoulos ,
C. Boscos ,
Ph. Saratsis ,
C. Saoulidis  and
S. Kyriakis
C. Alexopoulos
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
C. Boscos
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
Ph. Saratsis
Affiliation:
Clinic of Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
C. Saoulidis
Affiliation:
Clinic of Medicine, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
S. Kyriakis
Affiliation:
Clinic of Medicine, Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, GR 54006 Thessaloniki, Greece
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Abstract

Semen collection from three boars was performed twice a week, and doses (100 ml each) of semen diluted with Beltsville Thaw Solution (BTS) extender were prepared containing 1 × 109, 3 × 109 and 5 × 109 spermatozoa. Diluted semen was then stored at 17°C for a maximum of 120 h. Percentage of motile spermatozoa (PMS) and type of spermatozoa motility (TSM) were determined by microscopic examinations performed at 24-h intervals, from zero time (beginning of storage at 17°C) up to 120 h of storage, and a sperm motility index (SMI) calculated. Sows (no. = 360) were divided into nine experimental groups (40 sows per group) and a combination of number of spermatozoa per dose and storage time was used for artificial insemination in each group. All the animals were inseminated twice at 12 and 36 h after detection of standing oestrus by a teaser boar. Return and farrowing data including litter size were recorded. PMS, TSM and SMI decreased significantly (P < 0·01) from 48 h up to 120 h of storage, this being more marked in semen doses of 5 × 109 spermatozoa. When semen stored for up to 24 h was used, no significant differences (P > 0·05) regarding return rates and farrowing rates were observed among the three groups inseminated with semen containing different numbers of spermatozoa per dose. Inseminations with semen doses of 1 × 109 and 3 × 109 spermatozoa stored for 48 to 72 h have significantly lower return rates (P < 0·05) and significantly higher farrowing rates (P < 0·05) than with doses of 5 × 109 spermatozoa. Inseminations with semen stored for more than 72 h gave relatively higher return rates and lower farrowing rates. Semen storage time and number of spermatozoa per dose appeared to reduce litter size only where semen doses of 5 X109 spermatozoa were inseminated after storage for more than 48 h.

Keywords

boarssemen diluentsspermatozoastorage life
Type
Research Article
Information
Animal Science , Volume 62 , Issue 3 , June 1996 , pp. 599 - 604
Copyright
Copyright © British Society of Animal Science 1996

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