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Chapter 7 - Embryo Assisted Hatching for IVF

Training Protocol and Method

from Section 1 - Starting a New Laboratory and Training Protocols

Published online by Cambridge University Press:  11 May 2017

By
Mina Alikani
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Fertility Associates, New Zealand
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Type
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Information
Principles of IVF Laboratory Practice
Optimizing Performance and Outcomes
 , pp. 44 - 52
Publisher: Cambridge University Press
Print publication year: 2017

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References

McLaren, A. Can mouse blastocysts stimulate a uterine response before losing the zona pellucida? J Reprod Fertil 1969; 19:199–201.Google Scholar
Gonzales, D. S. and Bavister, B. D. Zona pellucida escape by hamster blastocysts in vitro is delayed and morphologically different compared with zona escape in vivo. Biol Reprod 1995; 52(2):470–80.CrossRefGoogle ScholarPubMed
Montag, M., Koll, B., Holmes, P. and van der Ven, H. Significance of the number of embryonic cells and the state of the zona pellucida for hatching of mouse blastocysts in vitro versus in vivo. Biol Reprod 2000; 62(6):1738–44.CrossRefGoogle ScholarPubMed
Malter, H. E. and Cohen, J. Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos. Gamete Res 1989; 24:67–80.Google Scholar
Cohen, J., Alikani, M., Trowbridge, J. and Rosenwaks, Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod 1992; 7:685–91.Google Scholar
Cohen, J. Assisted hatching: indications and techniques. Acta Eur Fertil 1993; 24:215–19.Google ScholarPubMed
Schimmel, T., Cohen, J., Saunders, H. and Alikani, M. Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse. Hum Reprod 2014; 29:2670–9.CrossRefGoogle Scholar
Cohen, J. and Feldberg, D. Effects of the size and number of zona pellucida openings on hatching and trophoblast outgrowth in the mouse embryo. Mol Reprod Dev 1991; 30:70–8.Google Scholar
Carney, S. K., Das, S., Blake, D., Farquhar, C., Seif, M. M. et al. Assisted hatching on assisted conception (in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI)). Cochrane Database Syst Rev 2012; 12:CD001894.Google ScholarPubMed
Martins, W. P., Rocha, I. A., Ferriani, R. A. and Nastri, C. O. Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials. Hum Reprod Update 2011; 17:438–53.Google Scholar
Practice Committee of the American Society for Reproductive Medicine and Practice Committee of the Society for Assisted Reproductive Technology. Role of assisted hatching in in vitro fertilization: a guideline. Fertil Steril 2014; 102:348–51.Google Scholar
Singh, H., Nardo, L., Kimber, S. J. and Aplin, J. D. Early stages of implantation as revealed by an in vitro model. Reproduction 2010; 139:905–14.CrossRefGoogle ScholarPubMed
Vajta, G., Rienzi, L. and Bavister, B. D. Zona-free embryo culture: is it a viable option to improve pregnancy rates? Reprod Biomed Online 2010; 21(1):17–25.Google Scholar
Ge, H. S., Zhou, W., Zhang, W. and Lin, J. J. Impact of assisted hatching on fresh and frozen-thawed embryo transfer cycles: a prospective, randomized study. Reprod Biomed Online 2008; 16:589–96.Google Scholar
Cohen, J. and Alikani, M. Evidence-based medicine and its application in clinical preimplantation embryology. Reprod Biomed Online 2013; 27:547–61.Google Scholar
Chailert, C., Sanmee, U., Piromlertamorn, W., Samchimchom, S. and Vutyavanich, T. Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers. Reprod Biol Endocrinol 2013; 11:21.Google Scholar
Douglas-Hamilton, D. H. and Conia, J. Thermal effects in laser-assisted pre-embryo zona drilling. J Biomed Opt 2001; 6(2):205–13.Google Scholar

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