Mechanisms of homologous recombination in bacteria

doi:10.1017/CBO9780511541544.001

1 - Mechanisms of homologous recombination in bacteria

Published online by Cambridge University Press: 06 August 2009

Marie-Agnès Petit

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Peter Mullany

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Marie-Agnès Petit: Affiliation:
Faculté de médecine Necker-Enfants Malades, France
Peter Mullany: Affiliation:
University College London

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Summary

Homologous recombination promotes the pairing between identical — or nearly identical – DNA sequences and the subsequent exchange of genetic material between them. It is an important and widely conserved function in living organisms, from bacteria to humans, that serves to repair double-stranded breaks or single-stranded gaps in the DNA, arising as a consequence of ionizing radiations, ultraviolet (UV) light, or chemical treatments creating replication-blocking adducts (Kuzminov, 1999). More recently, homologous recombination functions were also found in bacteria to rescue replication forks that have stalled for various reasons, such as a missing factor (e.g., the helicase), or a particular difficulty upstream of the fork, such as supercoiling or intense traffic of proteins (Michel et al., 2001).

Besides its molecular role, homologous recombination has played a major role in genome dynamics, by changing gene copy numbers through deletions, duplications, and amplifications: Intrachromosomal recombination between ribosomal operons or between mobile elements scattered into the genome leads to deletion or tandem duplications of large regions within the genome, up to several hundred kilobases (Roth et al., 1996). The duplications are unstable. Mostly they recombine back to the parental organization, and, therefore, remain undetected, except when appropriate selection, by gene dosage mostly, is exerted (Petes and Hill, 1988). In contrast, such duplications are ideal substrate for the diversification of genes: One gene is kept intact whereas the other is mutagenized, which leads to the birth of gene families.

Type: Chapter
Information: The Dynamic Bacterial Genome , pp. 3 - 32

DOI: https://doi.org/10.1017/CBO9780511541544.001 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2005

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References

Abdulkarim, F., and Hughes, D. (1996). Homologous recombination between the tuf genes of Salmonella typhimurium. J Mol Biol, 260, 506–522CrossRef Google Scholar PubMed

Alonso, J. C., Luder, G., and Tailor, R. H. (1991). Characterization of Bacillus subtilis recombinational pathways. J Bacteriol, 173, 3977–3980CrossRef Google Scholar PubMed

Amundsen, S. K., and Smith, G. R. (2003). Interchangeable parts of the Escherichia coli recombination machinery. Cell, 112, 741–744CrossRef Google Scholar PubMed

Anderson, D. G., and Kowalczykowski, S. C. (1997). The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a chi-regulated manner. Cell, 90, 77–86CrossRef Google Scholar

Anderson, R. P., and Roth, J. R. (1977). Tandem genetic duplications in phage and bacteria. Annu Rev Microbiol, 31, 473–505CrossRef Google Scholar PubMed

Ayora, S., Missich, R., Mesa, P., Lurz, R., Yang, S., Egelman, E. H., and Alonso, J. C. (2002). Homologous-pairing activity of the Bacillus subtilis bacteriophage SPP1 replication protein G35P. J Biol Chem, 277, 35969–35979CrossRef Google Scholar PubMed

Ayora, S., Carrasco, B., Doncel, E., Lurz, R., and Alonso, J. C. (2004). Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA. Proc Natl Acad Sci U S A, 101, 452–457CrossRef Google Scholar PubMed

Baitin, D. M., Zaitsev, E. N., and Lanzov, V. A. (2003). Hyper-recombinogenic RecA protein from Pseudomonas aeruginosa with enhanced activity of its primary DNA binding site. J Mol Biol, 328, 1–7CrossRef Google Scholar PubMed

Bierne, H., Seigneur, M., Ehrlich, S. D., and Michel, B. (1997). uvrD mutations enhance tandem repeat deletion in the Escherichia coli chromosome via SOS induction of the RecF recombination pathway. Mol Microbiol, 26, 557–567CrossRef Google Scholar PubMed

Bleuit, J. S., Xu, H., Ma, Y., Wang, T., Liu, J., and Morrical, S. W. (2001). Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A, 98, 8298–8305CrossRef Google Scholar PubMed

Bolt, E. L., and Lloyd, R. G. (2002). Substrate specificity of RusA resolvase reveals the DNA structures targeted by RuvAB and RecG in vivo. Mol Cell, 10, 187–198CrossRef Google Scholar PubMed

Bruand, C., Farache, M., McGovern, S., Ehrlich, S. D., and Polard, P. (2001). DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome. Mol Microbiol, 42, 245–255CrossRef Google Scholar PubMed

Buchmeier, N. A., Lipps, C. J., So, M. Y., and Heffron, F. (1993). Recombination-deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages. Mol Microbiol, 7, 933–936CrossRef Google Scholar PubMed

Canceill, D., and Ehrlich, S. D. (1996). Copy-choice recombination mediated by DNA polymerase III holoenzyme from Escherichia coli. Proc Natl Acad Sci U S A, 93, 6647–6652CrossRef Google Scholar PubMed

Capaldo, F. N., Ramsey, G., and Barbour, S. D. (1974). Analysis of the growth of recombination-deficient strains of Escherichia coli K-12. J Bacteriol, 118, 242–249Google Scholar PubMed

Chedin, F., Ehrlich, S. D., and Kowalczykowski, S. C. (2000). The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro. J Mol Biol, 298, 7–20CrossRef Google Scholar PubMed

Chedin, F., and Kowalczykowski, S. C. (2002). A novel family of regulated helicases/nucleases from gram-positive bacteria: Insights into the initiation of DNA recombination. Mol Microbiol, 43, 823–834CrossRef Google Scholar PubMed

Courcelle, J., Khodursky, A., Peter, B., Brown, P. O., and Hanawalt, P. C. (2001). Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics, 158, 41–64Google Scholar PubMed

Cox, M. M. (2001). Recombinational DNA repair of damaged replication forks in Escherichia coli: Questions. Annu Rev Genet, 35, 53–82CrossRef Google Scholar PubMed

Cromie, G. A., Connelly, J. C., and Leach, D. R. (2001). Recombination at double-strand breaks and DNA ends: Conserved mechanisms from phage to humans. Mol Cell, 8, 1163–1174CrossRef Google Scholar

Cromie, G. A., and Leach, D. R. (2000). Control of crossing over. Mol Cell, 6, 815–826CrossRef Google Scholar PubMed

d'Alencon, E., Petranovic, M., Michel, B., Noirot, P., Aucouturier, A., Uzest, M., and Ehrlich, S. D. (1994). Copy-choice illegitimate DNA recombination revisited. Embo J, 13, 2725–2734Google Scholar PubMed

Dale, C., Wang, B., Moran, N., and Ochman, H. (2003). Loss of DNA recombinational repair enzymes in the initial stages of genome degeneration. Mol Biol Evol, 20, 1188–1194CrossRef Google Scholar PubMed

Datsenko, K. A., and Wanner, B. L. (2000). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A, 97, 6640–6645CrossRef Google Scholar PubMed

Vos, W. M., Vries, S. C., and Venema, G. (1983). Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis. Gene, 25, 301–308CrossRef Google Scholar PubMed

Denamur, E., Bonacorsi, S., Giraud, A., Duriez, P., Hilali, F., Amorin, C., Bingen, E.. (2002). High frequency of mutator strains among human uropathogenic Escherichia coli isolates. J Bacteriol, 184, 605–609CrossRef Google Scholar PubMed

Dillingham, M. S., Spies, M., and Kowalczykowski, S. C. (2003). RecBCD enzyme is a bipolar DNA helicase. Nature, 423, 893–897CrossRef Google Scholar PubMed

Dubnau, D., and Lovett, C. M., Jr. (2002). “Transformation and recombination.” In Sonenshein, A. L., Hoch, J. A., and Losick, R. (eds.), Bacillus subtilis and its closest relatives. From genes to cells. Washington, DC: ASM Press, pp. 453–471Google Scholar

Earl, A. M., Mohundro, M. M., Mian, I. S., and Battista, J. R. (2002). The IrrE protein of Deinococcus radiodurans R1 is a novel regulator of recA expression. J Bacteriol, 184, 6216–6224CrossRef Google Scholar PubMed

el Karoui, M., Biaudet, V., Schbath, S., and Gruss, A. (1999). Characteristics of Chi distribution on different bacterial genomes. Res Microbiol, 150, 579–587CrossRef Google Scholar PubMed

el Karoui, M., Ehrlich, D., and Gruss, A. (1998). Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence. Proc Natl Acad Sci U S A, 95, 626–631CrossRef Google Scholar PubMed

Fernandez, S., Ayora, S., and Alonso, J. C. (2000). Bacillus subtilis homologous recombination: genes and products. Res Microbiol, 151, 481–486CrossRef Google Scholar PubMed

Fernandez De Henestrosa, A. R., Ogi, T., Aoyagi, S., Chafin, D., Hayes, J. J., Ohmori, H., and Woodgate, R. (2000). Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol Microbiol, 35, 1560–1572CrossRef Google Scholar PubMed

Forterre, (1999). Displacement of cellular proteins by functional analogues from plasmids or viruses could explain puzzling phylogenies of many DNA informational proteins. Mol Microbiol 33, 457–465CrossRef Google Scholar PubMed

Hall, S. D., and Kolodner, R. D. (1994). Homologous pairing and strand exchange promoted by the Escherichia coli RecT protein. Proc Natl Acad Sci U S A, 91, 3205–3209CrossRef Google Scholar PubMed

Halpern, D., Gruss, A., Claverys, J. P., and El-Karoui, M. (2004). rexAB mutants in Streptococcus pneumoniae. Microbiology, 150, 2409–2414CrossRef Google Scholar PubMed

Hedayati, M. A., Steffen, S. E., and Bryant, F. R. (2002). Effect of the Streptococcus pneumoniae MmsA protein on the RecA protein-promoted three-strand exchange reaction. Implications for the mechanism of transformational recombination. J Biol Chem, 277, 24863–24869CrossRef Google Scholar PubMed

Hsieh, P., Camerini-Otero, C. S., and Camerini-Otero, R. D. (1992). The synapsis event in the homologous pairing of DNAs: RecA recognizes and pairs less than one helical repeat of DNA. Proc Natl Acad Sci U S A, 89, 6492–6496CrossRef Google Scholar PubMed

Humbert, O., Prudhomme, M., Hakenbeck, R., Dowson, C. G., and Claverys, J. P. (1995). Homeologous recombination and mismatch repair during transformation in Streptococcus pneumoniae: Saturation of the Hex mismatch repair system. Proc Natl Acad Sci U S A, 92, 9052–9056CrossRef Google Scholar PubMed

Ivancic-Bace, I., Peharec, P., Moslavac, S., Skrobot, N., Salaj-Smic, E., and Brcic-Kostic, K. (2003). RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli. Genetics, 163, 485–494Google Scholar

Kim, J. I., and Cox, M. M. (2002). The RecA proteins of Deinococcus radiodurans and Escherichia coli promote DNA strand exchange via inverse pathways. Proc Natl Acad Sci U S A, 99, 7917–7921CrossRef Google Scholar PubMed

Kodama, K., Kobayashi, T., Niki, H., Hiraga, S., Oshima, T., Mori, H., and Horiuchi, T. (2002). Amplification of hot DNA segments in Escherichia coli. Mol Microbiol, 45, 1575–1588CrossRef Google Scholar PubMed

Kogoma, T., Cadwell, G. W., Barnard, K. G., and Asai, T. (1996). The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair. J Bacteriol, 178, 1258–1264CrossRef Google Scholar PubMed

Kooistra, J., Haijema, B. J., and Venema, G. (1993). The Bacillus subtilis addAB genes are fully functional in Escherichia coli. Mol Microbiol, 7, 915–923CrossRef Google Scholar PubMed

Kowalczykowski, S. C., Dixon, D. A., Eggleston, A. K., Lauder, S. D., and Rehrauer, W. M. (1994). Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev, 58, 401–465Google Scholar PubMed

Kuzminov, A. (1999). Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol Mol Biol Rev, 63, 751–813Google Scholar PubMed

Lloyd, R. G. (1991). Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG. J Bacteriol, 173, 5414–5418CrossRef Google Scholar PubMed

Lloyd, R. G., and Low, K. B. (1996). “Homologous recombination.” In Neidhart, F. C. (ed.), Escherichia coli and Salmonella, Vol. 2. Washington, DC: ASM Press, pp. 2236–2255Google Scholar

Lloyd, R. G., and Sharples, G. J. (1993). Dissociation of synthetic Holliday junctions by E. coli RecG protein. EMBO J, 12, 17–22Google Scholar PubMed

Loughlin, M. F., Barnard, F. M., Jenkins, D., Sharples, G. J., and Jenks, P. J. (2003). Helicobacter pylori mutants defective in RuvC Holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa. Infect Immun, 71, 2022–2031CrossRef Google Scholar PubMed

Lovett, C. M. Jr., Love, P. E., and Yasbin, R. E. (1989). Competence-specific induction of the Bacillus subtilis RecA protein analog: Evidence for dual regulation of a recombination protein. J Bacteriol, 171, 2318–2322CrossRef Google Scholar PubMed

Majewski, J., and Cohan, F. M. (1998). The effect of mismatch repair and heteroduplex formation on sexual isolation in Bacillus. Genetics, 148, 13–18Google Scholar PubMed

Marians, K. J. (1999). PriA: At the crossroads of DNA replication and recombination. Prog Nucleic Acid Res Mol Biol, 63, 39–67CrossRef Google Scholar

Marians, K. J. (2000). PriA-directed replication fork restart in Escherichia coli. Trends Biochem Sci, 25, 185–189CrossRef Google Scholar PubMed

Marsin, S., McGovern, S., Ehrlich, S. D., Bruand, C., and Polard, P. (2001). Early steps of Bacillus subtilis primosome assembly. J Biol Chem, 276, 45818–45825CrossRef Google Scholar PubMed

Martin, B., Sharples, G. J., Humbert, O., Lloyd, R. G., and Claverys, J. P. (1996). The mmsA locus of Streptococcus pneumoniae encodes a RecG-like protein involved in DNA repair and in three-strand recombination. Mol Microbiol, 19, 1035–1045CrossRef Google Scholar PubMed

McGlynn, P., and Lloyd, R. G. (2000). Modulation of RNA polymerase by (p)ppGpp reveals a RecG-dependent mechanism for replication fork progression. Cell, 101, 35–45CrossRef Google Scholar PubMed

Michel, B., Flores, M. J., Viguera, E., Grompone, G., Seigneur, M., and Bidnenko, V. (2001). Rescue of arrested replication forks by homologous recombination. Proc Natl Acad Sci U S A, 98, 8181–8188CrossRef Google Scholar PubMed

Michel, B., Recchia, G. D., Penel-Colin, M., Ehrlich, S. D., and Sherratt, D. J. (2000). Resolution of Holliday junctions by RuvABC prevents dimer formation in rep mutants and UV-irradiated cells. Mol Microbiol, 37, 180–191CrossRef Google Scholar PubMed

Miller, E. S., Kutter, E., Mosig, G., Arisaka, F., Kunisawa, T., and Ruger, W. (2003). Bacteriophage T4 genome. Microbiol Mol Biol Rev, 67, 86–156CrossRef Google Scholar PubMed

Morimatsu, K., and Kowalczykowski, S. C. (2003). RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: A universal step of recombinational repair. Mol Cell, 11, 1337–1347CrossRef Google Scholar PubMed

Muyrers, J. P., Zhang, Y., Buchholz, F., and Stewart, A. F. (2000). RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners. Genes Dev, 14, 1971–1982Google Scholar PubMed

Myers, R. S., and Stahl, F. W. (1994). Chi and the RecBC D enzyme of Escherichia coli. Annu Rev Genet, 28, 49–70CrossRef Google Scholar PubMed

Narumi, I., Satoh, K., Kikuchi, M., Funayama, T., Yanagisawa, T., Kobayashi, Y., Watanabe, H., and Yamamoto, K. (2001). The LexA protein from Deinococcus radiodurans is not involved in RecA induction following gamma irradiation. J Bacteriol, 183, 6951–6956CrossRef Google Scholar

Noirot, P., Gupta, R. C., Radding, C. M., and Kolodner, R. D. (2003). Hallmarks of homology recognition by RecA-like recombinases are exhibited by the unrelated Escherichia coli RecT protein. Embo J, 22, 324–334CrossRef Google Scholar PubMed

Noirot, P., and Kolodner, R. D. (1998). DNA strand invasion promoted by Escherichia coli RecT protein. J Biol Chem, 273, 12274–12280CrossRef Google Scholar PubMed

Ochman, H., Lawrence, J. G., and Groisman, E. A. (2000). Lateral gene transfer and the nature of bacterial innovation. Nature, 405, 299–304CrossRef Google Scholar PubMed

Ogura, M., Yamaguchi, H., Kobayashi, K., Ogasawara, N., Fujita, Y., and Tanaka, T. (2002). Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. J Bacteriol, 184, 2344–2351CrossRef Google Scholar PubMed

Petes, T. D., and Hill, C. W. (1988). Recombination between repeated genes in microorganisms. Annu Rev Genet, 22, 147–168CrossRef Google Scholar PubMed

Petit, M. A., Dimpfl, J., Radman, M., and Echols, H. (1991). Control of large chromosomal duplications in Escherichia coli by the mismatch repair system. Genetics, 129, 327–332Google Scholar PubMed

Polard, P., Marsin, S., McGovern, S., Velten, M., Wigley, D. B., Ehrlich, S. D., and Bruand, C. (2002). Restart of DNA replication in gram-positive bacteria: Functional characterisation of the Bacillus subtilis PriA initiator. Nucleic Acids Res, 30, 1593–1605CrossRef Google Scholar PubMed

Quiberoni, A., Biswas, I., El Karoui, M., Rezaiki, L., Tailliez, P., and Gruss, A. (2001). In vivo evidence for two active nuclease motifs in the double-strand break repair enzyme RexAB of Lactococcus lactis. J Bacteriol, 183, 4071–4078CrossRef Google Scholar PubMed

Rayssiguier, C., Thaler, D. S., and Radman, M. (1989). The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature, 342, 396–401CrossRef Google Scholar PubMed

Rinken, R., and Wackernagel, W. (1992). Inhibition of the recBCD-dependent activation of Chi recombinational hot spots in SOS-induced cells of Escherichia coli. J Bacteriol, 174, 1172–1178CrossRef Google Scholar PubMed

Robu, M. E., Inman, R. B., and Cox, M. M. (2001). RecA protein promotes the regression of stalled replication forks in vitro. Proc Natl Acad Sci U S A, 98, 8211–8218CrossRef Google Scholar PubMed

Roca, A. I., and Cox, M. M. (1997). RecA protein: Structure, function, and role in recombinational DNA repair. Prog Nucleic Acid Res Mol Biol, 56, 129–223CrossRef Google Scholar PubMed

Roth, J. R., Benson, N., Galitski, T., Haak, K., Lawrence, J., and Miesel, L. (1996). “Rearrangements of the bacterial chromosome: Formation and applications.” In Neidhart, F. C. (ed.), Escherichia coli and Salmonella, Vol. 2. Washington, DC: ASM Press, pp. 2256–2276Google Scholar

Saveson, C. J., and Lovett, S. T. (1997). Enhanced deletion formation by aberrant DNA replication in Escherichia coli. Genetics, 146, 457–470Google Scholar PubMed

Sawitzke, J. A., and Stahl, F. W. (1997). Roles for lambda Orf and Escherichia coli RecO, RecR and RecF in lambda recombination. Genetics, 147, 357–369Google Scholar PubMed

Schapiro, J. M., Libby, S. J., and Fang, F. C. (2003). Inhibition of bacterial DNA replication by zinc mobilization during nitrosative stress. Proc Natl Acad Sci U S A, 100, 8496–8501CrossRef Google Scholar PubMed

Schmitt, W., Odenbreit, S., Heuermann, D., and Haas, R. (1995). Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Mol Gen Genet, 248, 563–572CrossRef Google Scholar PubMed

Seigneur, M., Bidnenko, V., Ehrlich, S. D., and Michel, B. (1998). RuvAB acts at arrested replication forks. Cell, 95, 419–430CrossRef Google Scholar PubMed

Seigneur, M., Ehrlich, S. D., and Michel, B. (2000). RuvABC-dependent double-strand breaks in dnaBts mutants require recA. Mol Microbiol, 38, 565–574CrossRef Google Scholar PubMed

Sharples, G. J. (2001). The X philes: Structure-specific endonucleases that resolve Holliday junctions. Mol Microbiol, 39, 823–834CrossRef Google Scholar PubMed

Sharples, G. J., Ingleston, S. M., and Lloyd, R. G. (1999). Holliday junction processing in bacteria: Insights from the evolutionary conservation of RuvABC, RecG, and RusA. J Bacteriol, 181, 5543–5550Google Scholar PubMed

Singleton, M. R., Dillingham, M. S., Gaudier, M., Kowalczykowski, S. C., and Wigley, D. B. (2004). Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature, 432, 187–193CrossRef Google Scholar PubMed

Takahashi, N. K., Kusano, K., Yokochi, T., Kitamura, Y., Yoshikura, H., and Kobayashi, I. (1993). Genetic analysis of double-strand break repair in Escherichia coli. J Bacteriol, 175, 5176–5185CrossRef Google Scholar PubMed

Tatusov, R. L., Koonin, E. V., and Lipman, D. J. (1997). A genomic perspective on protein families. Science, 278, 631–637CrossRef Google Scholar PubMed

Tatusov, R. L., Natale, D. A., Garkavtsev, I. V., Tatusova, T. A., Shankavaram, U. T., Rao, B. S., Kiryutin, B., Galperin, M. Y., Fedorova, N. D., and Koonin, E. V. (2001). The COG database: New developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res, 29, 22–28CrossRef Google Scholar PubMed

Taylor, A. F., and Smith, G. R. (2003). RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarity. Nature, 423, 889–893CrossRef Google Scholar PubMed

Velten, M., McGovern, S., Marsin, S., Ehrlich, S. D., Noirot, P., and Polard, P. (2003). A two-protein strategy for the functional loading of a cellular replicative DNA helicase. Mol Cell, 11, 1009–1020CrossRef Google Scholar PubMed

Viguera, E., Canceill, D., and Ehrlich, S. D. (2001). Replication slippage involves DNA polymerase pausing and dissociation. EMBO J, 20, 2587–2595CrossRef Google Scholar PubMed

Voloshin, O. N., Ramirez, B. E., Bax, A., and Camerini-Otero, R. D. (2001). A model for the abrogation of the SOS response by an SOS protein: A negatively charged helix in DinI mimics DNA in its interaction with RecA. Genes Dev, 15, 415–427CrossRef Google Scholar PubMed

Vulic, M., Dionisio, F., Taddei, F., and Radman, M. (1997). Molecular keys to speciation: DNA polymorphism and the control of genetic exchange in enterobacteria. Proc Natl Acad Sci U S A, 94, 9763–9767CrossRef Google Scholar PubMed

Xu, L., and Marians, K. J. (2003). PriA mediates DNA replication pathway choice at recombination intermediates. Mol Cell, 11, 817–826CrossRef Google Scholar PubMed

Yu, D., Ellis, H. M., Lee, E. C., Jenkins, N. A., Copeland, N. G., and Court, D. L. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U S A, 97, 5978–5983CrossRef Google Scholar PubMed

Zhang, Y., Buchholz, F., Muyrers, J. P., and Stewart, A. F. (1998). A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet, 20, 123–128CrossRef Google Scholar PubMed

Zulty, J. J., and Barcak, G. J. (1993). Structural organization, nucleotide sequence, and regulation of the Haemophilus influenzae rec-1+ gene. J Bacteriol, 175, 7269–7281CrossRef Google Scholar PubMed

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