Book contents
- Frontmatter
- Contents
- Introduction
- How important is maths in data-handling?
- Abbreviations and the Système International
- Acknowledgements
- 1 Numbers and indices
- 2 A sense of proportion
- 3 Graphs
- 4 Algebra
- 5 Logarithms: exponential and logarithmic functions
- 6 Simple statistics
- 7 Preparing solutions and media
- 8 Enzymes
- 9 Spectrophotometry
- 10 Energy metabolism
- 11 Radioactivity
- 12 Growth in batch cultures
- 13 Growth in continuous culture
- 14 Microbial genetics
- 15 Problems
- 16 Advice and hints
- 17 Answers to problems
- Conclusion
- Further reading
- Index
9 - Spectrophotometry
Published online by Cambridge University Press: 05 June 2012
- Frontmatter
- Contents
- Introduction
- How important is maths in data-handling?
- Abbreviations and the Système International
- Acknowledgements
- 1 Numbers and indices
- 2 A sense of proportion
- 3 Graphs
- 4 Algebra
- 5 Logarithms: exponential and logarithmic functions
- 6 Simple statistics
- 7 Preparing solutions and media
- 8 Enzymes
- 9 Spectrophotometry
- 10 Energy metabolism
- 11 Radioactivity
- 12 Growth in batch cultures
- 13 Growth in continuous culture
- 14 Microbial genetics
- 15 Problems
- 16 Advice and hints
- 17 Answers to problems
- Conclusion
- Further reading
- Index
Summary
And when we consider that other theory of the natural philosophers, that all other earthly hues – every stately or lovely emblazoning – the sweet tinges of skies and woods; yea, and the gilded velvets of butterflies, and the butterfly cheeks of young girls; all these are but subtile deceits, not actually inherent in substances, but only laid on from without; so that all deified Nature absolutely paints like the harlot, whose allurements cover nothing but the charnel-house within; and when we proceed further, and consider that the mystical cosmetic which produces every one of her hues, the great principle of light, for ever remains white or colorless in itself, and if operating without medium upon matter, would touch all objects, even tulips and roses, with its own blank tinge –
Herman MelvilleThree commonplace observations are the basis of this subject:
(1) A stronger solution of a coloured substance looks darker than does a weaker solution of that substance.
(2) A thin layer of a coloured solution is paler than a thicker layer of the same solution.
(3) Different substances can give solutions of different colours.
Use of the spectrophotometer
These observations are made quantitative by using a spectrophotometer. In this machine a beam of light from an electric bulb (wavelengths about 350 to 800 nm) or from a source of UV (wavelengths about 220 to 350 nm) passes through a solution.
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- Information
- Data-Handling in Biomedical Science , pp. 87 - 98Publisher: Cambridge University PressPrint publication year: 2010