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RA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos

Published online by Cambridge University Press:  23 September 2022

Tais Aparecida Patrocinio ,
Carlos Antônio de Carvalho Fernandes Open the ORCID record for Carlos Antônio de Carvalho Fernandes [Opens in a new window] ,
Gilson Costa Macedo ,
Adilson David da Silva ,
Nadia Rezende Raposo Barbosa ,
Carolina Capobiango Romano Quintao ,
Marcela Eduarda da Silva Ribeiro  and
Luiz Sergio Almeida Camargo
Tais Aparecida Patrocinio
Affiliation:
UNIFENAS. Rodovia MG 179 – Km 0. C.P. 37130-000, Minas Gerais, Brazil
Carlos Antônio de Carvalho Fernandes*
Affiliation:
UNIFENAS. Rodovia MG 179 – Km 0. C.P. 37130-000, Minas Gerais, Brazil BIOTRAN, Rua Tatuin 447, C.P. 37130-000, MG, Brazil
Gilson Costa Macedo
Affiliation:
Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil
Adilson David da Silva
Affiliation:
Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil
Nadia Rezende Raposo Barbosa
Affiliation:
Universidade Federal de Juiz de Fora, UFJF, Rua José Lourenço Kelmer, 36036-900, MG, Brazil
Carolina Capobiango Romano Quintao
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA Gado de Leite, C.P. 36038-330, MG, Brazil
Marcela Eduarda da Silva Ribeiro
Affiliation:
UNIFENAS. Rodovia MG 179 – Km 0. C.P. 37130-000, Minas Gerais, Brazil
Luiz Sergio Almeida Camargo
Affiliation:
UNIFENAS. Rodovia MG 179 – Km 0. C.P. 37130-000, Minas Gerais, Brazil Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA Gado de Leite, C.P. 36038-330, MG, Brazil
*
Author for correspondence: Carlos Antônio de Carvalho Fernandes. BIOTRAN. Rua Tatuin 447. C.P. 37130-000. MG. Brazil. E-mail: [email protected]
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Summary

Oxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro-fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher (P < 0.05) with 2.5 μM (69.0 ± 4.4%) than at 0, 0.1 or 0.5 μM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 μM RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher (P < 0.05) than for 0 μM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 μM RA33 was compared with 0.5 μM resveratrol, similar (P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher (P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower (P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro-fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium.

Keywords

AntioxidantApoptosisEmbryo cultureIn vitro cultureOxidative stress
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 891 - 894
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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