Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-30T23:13:20.004Z Has data issue: false hasContentIssue false
  • English
  • Français

Laser blastocyst biopsy for preimplantation diagnosis in the human

Published online by Cambridge University Press:  26 September 2008

A. Veiga ,
M. Sandalinas ,
M. Benkhalifa ,
M. Boada ,
M. Carrera ,
J. Santaló ,
P.N. Barri  and
Y. Ménézo
A. Veiga*
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Sandalinas
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Benkhalifa
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Boada
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
M. Carrera
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
J. Santaló
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
P.N. Barri
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
Y. Ménézo
Affiliation:
Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France
*
A. Veiga, Reproductive Medicine Service, Department of Obstetrics and Gynaecology, Institut Universitari Dexeus, Barcelona, Spain.
Get access Rights & Permissions [Opens in a new window]

Summary

A new methodology for blastocyst biopsy that uses a 1.48μm in diode laser is described. Trophectoderm cells are biopsied after laster zona drilling and culture, fixed and processed for fluorescent in situ hybridisation (FISH) analysis. Preliminary results on the efficiency of the procedure and blastocyst recovery rate are promising. Blastocyst laser biopsy is a useful tool in preimplantation genetic diagnosis (PGD) as it allows a more reliable diagnosis and widens the diagnostic possibilities on account of the higher number of cells obtained in the biopsy.

Keywords

BlastocystNon-contact laserPreimplantation genetic diagnosisTrophectoderm biopsy
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 351 - 354
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Benkhalifa, M., Ménézo, Y., Janny, L., et al., (1996). Cytogenetics of uncleaved oocytes and arrested zygotes in IVF programmes. J. Assist. Reprod. Genet. 13, 140–8.CrossRefGoogle Scholar
Carson, S.A., Gentry, W.L. & Smith, A.L. (1993). Trophectoderm microbiopsy in murine blastocysts: comparison of four methods. J. Assist. Reprod. Genet. 10, 427–33.CrossRefGoogle ScholarPubMed
Dokras, A.Sargent, I.L. & Ross, C. (1990). Trophectoderm biopsy in human blastocysts. Hum. Reprod. 5, 821–5.CrossRefGoogle ScholarPubMed
Findlay, I., Ray, P., Quirke, P., Rutherford, A. & Lilford, R. (1995). Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis. Mol. Hum. Reprod. 10, 1609–18.CrossRefGoogle ScholarPubMed
Germond, M.Nocera, D., Senn, A., Rink, K., Delacrétaz, G. & Fakan, S. (1995). Microdissection of mouse and human zona pellucida using a 1.48 μm diode laser beam: efficiency and safety of the procedure. Fertil. Steril. 25, 604–11.CrossRefGoogle Scholar
Germond, M.Nocera, D., Senn, A., Rink, K., Delacrétaz, G., Pedrazzini, T. & Hornung, J.P. (1996). Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 μm diode laser beam. Hum. Reprod. 11, 1043–8.CrossRefGoogle Scholar
Handyside, A., Kontogianni, E., Hardy, K. et al. (1990). Pregnancies from biopsied human preimplantation embryos sexed with a Y-specific DNA amplification. Nature 344, 768–70.CrossRefGoogle ScholarPubMed
Handyside, A., Lesko, J., Tarín, J., Winston, R. & Hughes, M. (1992). Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis. N. Eng. J. Med. 327, 905–9.CrossRefGoogle ScholarPubMed
Hardy, K. & Handyside, A. (1992). Biopsy at the cleavage stage human embryos and diagnosis of single gene defects by DNA amplification. Arch. Pathol. Med. 116, 388–92.Google ScholarPubMed
Janny, L. & Ménézo, Y. (1994). Evidence of a strong paternal effect on human preimplantation embryo development and blastocyst formation. Mol. Reprod. Dev. 38, 36–42.CrossRefGoogle Scholar
Janny, L. & Ménézo, Y. (1996). Maternal age effect on early human embryonic development and blastocyst formation. Mol. Reprod. Dev. 45, 31–7.3.0.CO;2-T>CrossRefGoogle ScholarPubMed
Kaufmann, R.A., Servy, E. & Ménézo, Y. (1994). The use of blastocysts and coculture in advanced reproductive technologies. Assist. Reprod. Rev. 4, 192–4.Google Scholar
Ménézo, Y. & Benkhalifa, M. (1995). Cytogenetics and cryobiology of human cocultured embryos: a 3 year experience. J. Assist. Reprod. Genet. 12, 35–40.CrossRefGoogle Scholar
Ménézo, Y. & Veiga, A. (1997). Cryopreservation of blastocyst. In Proceedings of the Xth World Congress on In Vitro Fertilization and Assisted Rerproduction, Vancouver, pp. 49–53.Google Scholar
Ménézo, Y., Hazout, A., Dumont, M. et al. , (1992). Coculture of embryos on Vero cells and transfer at the blastocyst stage. Hum. Reprod. 7 (Suppl. 1), 101–6.CrossRefGoogle Scholar
Ménézo, Y., Kaufman, R., Nicollet, B. et al. , (1996). Efficiency of a simplified thawing protocol for human cocultured blastocysts. In Proceedings of the American Society for Reproductive Medicine, Boston, pp. 49–53.Google Scholar
Munné, S., Lee, A., Rosenwaks, Z., Grifo, J. & Cohen, J. (1993). Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum. Reprod. 8, 2185–91.CrossRefGoogle ScholarPubMed
Munné, S., Weier, H.U.G., Grifo, J. & Cohen, J. (1994). Chromosome mosaicism in human embryos. Biol. Reprod. 51, 373–9.CrossRefGoogle ScholarPubMed
Olivennes, F., Hazout, A., Lelaidier, C. et al. , (1994). Four indications for embryo transfer at the blastocyst stage. Hum. Reprod. 9, 2367–73.CrossRefGoogle ScholarPubMed
Rink, K., Delacrétaz, C., Salathé, R.P., Senn, A., Nocera, D., Germond, M., De Grandi, P. & Fakan, S. (1996). Non-contact microdrilling of mouse zona pellucida with an objective delivered 1.48 μm diode laser. Laser Surg. Med. 18, 52–62.3.0.CO;2-Q>CrossRefGoogle Scholar
Veiga, A., Santaló, J., Vidal, F., Calderón, G., Gimenez, C., Boada, M., Egozcue, J. & Barri, P.N. (1994). Twin pregnancy after implantation diagnosis for sex selection. Hum. Reprod. 9, 2156–9.CrossRefGoogle Scholar
Veiga, A., Torelló, M.J., Sandalinas, M., Busquets, A., Calderon, G. & Barri, P.N. (1995). Optimization of implantation in the in-vitro fertilization laboratory. Hum. Reprod. 10 Suppl. 2.98–106.CrossRefGoogle ScholarPubMed
Verlinsky, Y., Ginsberg, N., Lifchez, A. et al. , (1990). Analysis of the first plar body: preconception genetic diagnosis. Hum. Reprod. 5, 826–9.CrossRefGoogle Scholar