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FGF18 modulates CTGF mRNA expression in cumulus–oocyte complexes and early bovine embryos: preliminary data

Published online by Cambridge University Press:  18 August 2021

Elisabeth Schmidt da Silva ,
Carolina Amaral ,
Marcos Barreta ,
Alfredo Antoniazzi ,
Leonardo Guedes de Andrade Open the ORCID record for Leonardo Guedes de Andrade [Opens in a new window] ,
Rogério Ferreira ,
Fernando Mesquita ,
Valério Marques Portela  and
Paulo Bayard Gonçalves Open the ORCID record for Paulo Bayard Gonçalves [Opens in a new window]
Elisabeth Schmidt da Silva
Affiliation:
Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil
Carolina Amaral
Affiliation:
Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil
Marcos Barreta
Affiliation:
Laboratory of Animal Reproduction Physiology, Universidade Federal de Santa Catarina, SC, 89520-000, Brazil
Alfredo Antoniazzi
Affiliation:
Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil
Leonardo Guedes de Andrade
Affiliation:
Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil
Rogério Ferreira
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina, SC, 88035-901, Brazil
Fernando Mesquita
Affiliation:
Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil
Valério Marques Portela
Affiliation:
Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil
Paulo Bayard Gonçalves*
Affiliation:
Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil Laboratory of Animal Reproduction Physiology, Universidade Federal de Santa Catarina, SC, 89520-000, Brazil Department of Animal Science, Universidade do Estado de Santa Catarina, SC, 88035-901, Brazil Molecular and Integrative Physiology of Reproduction Laboratory, Universidade Federal do Pampa, Uruguaiana, RS, 97501-970, Brazil
*
Author for correspondence: Paulo Bayard Gonçalves. Biotechnology and Animal Reproduction Laboratory, Universidade Federal de Santa Maria, Santa Maria, RS, 97105–900, Brazil. E-mail: [email protected]
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Summary

The Hippo pathway is involved in the proliferation of intrafollicular cells and in early embryonic development, mainly because effectors of this pathway are key transcription regulators of genes such as CTGF and CYR61, which are involved in cell proliferation. Recent studies by our group found that fibroblast growth factor 18 (FGF18) is present in the fallopian tube during early embryonic development, leading to the hypothesis that FGF18 may have a role during embryonic development. Therefore, the aim of the following study was to determine whether FGF18 modulates the expression of Hippo pathway target genes, CTGF and CYR61, during oocyte maturation and early embryonic development. Three experiments were carried out, with in vitro maturation (IVM) of cumulus–oocyte complexes (COCs) and embryo culture. In experiment one, FGF18 (100 ng/ml) induced an increase (P < 0.05) in CTGF gene expression at 12 h post-exposure. In experiment two, FGF18 (100 ng/ml) induced a reduction (P < 0.05) in CTGF expression at 3 h post-exposure. In the third experiment, day 7 embryos exposed to FGF18 during oocyte IVM expressed greater CTGF mRNA abundance, whereas FGF18 exposure during embryo in vitro embryo culture did not alter CTGF expression in comparison with untreated controls. The preliminary data presented here show that FGF18 modulates CTGF expression in critical periods of oocyte nuclear maturation, cumulus expansion and early embryonic development in cattle.

Keywords

BovineCell proliferation genesEmbryonic developmentFibroblast growth factor 18Hippo Pathway
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 239 - 243
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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