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Expression of lncRNA TINCR in the placenta of patients with pre-eclampsia and its effect on the biological behaviours of trophoblasts

Published online by Cambridge University Press:  28 June 2021

Li Qin ,
Qin Yang ,
Zhiyi Fei  and
Dandan Zhang Open the ORCID record for Dandan Zhang [Opens in a new window]
Li Qin
Affiliation:
Obstetrics Department, Puren Hospital affiliated to Wuhan University of Science and Technology, Wuhan City, Hubei Province 430081, China
Qin Yang
Affiliation:
Reproductive Medicine Section, Puren Hospital affiliated to Wuhan University of Science and Technology, Wuhan City, Hubei Province 430081, China
Zhiyi Fei
Affiliation:
Obstetrics Department, Puren Hospital affiliated to Wuhan University of Science and Technology, Wuhan City, Hubei Province 430081, China
Dandan Zhang*
Affiliation:
Reproductive Medicine Section, Puren Hospital affiliated to Wuhan University of Science and Technology, Wuhan City, Hubei Province 430081, China
*
Author for correspondence: Dandan Zhang. Reproductive Medicine Section, Puren Hospital, affiliated to Wuhan University of Science and Technology, No. 1 Benxi Street, Qingshan District, Wuhan, 430081, Hubei Province, China. Tel: +86 27 86857375. E-mail: [email protected]
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Summary

To explore the effect of lncRNA TINCR on the biological behaviours of trophoblasts, we detected and analyzed the expression of terminal differentiation-induced non-protein coding RNA (TINCR) in the placenta tissues of pre-eclamptic and non-pre-eclamptic pregnant women. The gain- and loss-of-function of TINCR was performed to examine the proliferation, migration and invasion abilities of Htr-8/Svneo cells. The levels of epithelial–mesenchymal transition (EMT)-related proteins, cyclin and Wnt/β-catenin pathway were detected. High expression of lncRNA TINCR appeared in placental tissues of patients with pre-eclampsia. The proliferation, invasion and migration of Htr-8/Svneo cells were promoted by TINCR downregulation; the cells were transited from G0/G1 to S phase; and EMT was promoted and the Wnt/β-catenin pathway was activated. In summary, the downregulation of lncRNA TINCR activated the Wnt/β-catenin pathway and promoted the proliferation, invasion and migration of Htr-8/Svneo cells. This study may provide a theoretical basis for treatment of patients with pre-eclampsia.

Keywords

Cell cycleEpithelial–mesenchymal transitionHtr-8/SvneoInvasion and migrationlncRNA TINCRPre-eclampsiaProliferationWnt/β-catenin
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 111 - 119
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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