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Comparison of three antioxidants in chemical and biological assays on porcine oocytes during ageing in vitro

Published online by Cambridge University Press:  21 April 2022

Chan-Oh Park ,
Seung-Eun Lee ,
Jae-Wook Yoon ,
Hyo-Jin Park ,
So-Hee Kim ,
Seung-Hwan Oh ,
Do-Geon Lee ,
Da-Bin Pyeon ,
Eun-Young Kim  and
Se-Pill Park Open the ORCID record for Se-Pill Park [Opens in a new window]
Chan-Oh Park
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Seung-Eun Lee
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Jae-Wook Yoon
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Hyo-Jin Park
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
So-Hee Kim
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Seung-Hwan Oh
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Do-Geon Lee
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Da-Bin Pyeon
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea
Eun-Young Kim
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Mirae Cell Bio, Seongdong-gu, Seoul, Korea
Se-Pill Park*
Affiliation:
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Stem Cell Research Center, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, Korea Mirae Cell Bio, Seongdong-gu, Seoul, Korea
*
Author for correspondence: Se-Pill Park. Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Korea. Tel: +82 64 754 4650. Email: [email protected]
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Summary

Our previous studies have already revealed that β-cryptoxanthin (BCX), hesperetin (HES), and icariin (ICA) antioxidants are effective for in vitro maturation (IVM) of porcine oocytes. In this study, we investigated which of BCX, HES, or ICA was more effective for IVM of porcine oocytes. The antioxidant properties were assessed with aged porcine oocytes and embryos by comparing 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), reducing power, and H2O2 scavenging activity assays. The chemical assay results demonstrated that BCX had a greater DPPH scavenging activity and reducing power than HES and ICA, compared with controls. However, the H2O2 scavenging activity of the antioxidants was similar when tested at the optimal concentrations of 1 μM BCX (BCX-1), 100 μM HES (HES-100), and 5 μM ICA (ICA-5). The biological assay results showed that BCX-1 treatment was more effective in inducing a significant reduction in reactive oxygen species (ROS), improving glutathione levels, and increasing the expression of antioxidant genes. In addition, BCX-1 inhibited apoptosis by increasing the expression of anti-apoptotic genes and decreasing pro-apoptotic genes in porcine parthenogenetic blastocysts. BCX-1 also significantly increased the blastocyst formation rate compared with the ageing control group, HES-100 and ICA-5. This study demonstrates that damage from ROS produced during oocyte ageing can be prevented by supplementing antioxidants into the IVM medium, and BCX may be a potential candidate to improve assisted reproductive technologies.

Keywords

β-CryptoxanthinHesperetinIcariinIn vitro ageingPorcine oocyte
Type
Research Article
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 561 - 570
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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