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Advancements in mammalian X and Y sperm differences and sex control technology

Published online by Cambridge University Press:  28 February 2022

Qifu He Open the ORCID record for Qifu He [Opens in a new window] ,
Min Huang ,
Xuyang Cao ,
Kang Zhang ,
Jun Liu  and
Fusheng Quan
Qifu He
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
Min Huang
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
Xuyang Cao
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
Kang Zhang
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
Jun Liu*
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
Fusheng Quan*
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China Shaanxi Animal Embryo Engineering Technology Research Center, Yangling, Shaanxi, China
*
Authors for correspondence: Fusheng Quan. College of Veterinary Medicine, Northwest A&F University, Yangling712100, Shaanxi, China. Tel: +86 29 87080092. Fax: +86 2987080085. E-mail: [email protected]. Jun Liu. College of Veterinary Medicine, Northwest A&F University, Yangling712100, Shaanxi, China. Tel: +86 29 87080092. E-mail: [email protected].
Authors for correspondence: Fusheng Quan. College of Veterinary Medicine, Northwest A&F University, Yangling712100, Shaanxi, China. Tel: +86 29 87080092. Fax: +86 2987080085. E-mail: [email protected]. Jun Liu. College of Veterinary Medicine, Northwest A&F University, Yangling712100, Shaanxi, China. Tel: +86 29 87080092. E-mail: [email protected].
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Summary

Mammal sex determination depends on whether the X sperm or Y sperm binds to the oocyte during fertilization. If the X sperm joins in oocyte, the offspring will be female, if the Y sperm fertilizes, the offspring will be male. Livestock sex control technology has tremendous value for livestock breeding as it can increase the proportion of female offspring and improve the efficiency of livestock production. This review discusses the detailed differences between mammalian X and Y sperm with respect to their morphology, size, and motility in the reproductive tract and in in vitro conditions, as well as ’omics analysis results. Moreover, research progress in mammalian sex control technology has been summarized.

Keywords

Livestock’Omics researchSex controlSperm separationX spermatozoaY spermatozoa
Type
Review Article
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 423 - 430
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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References

Ali, JI, Eldridge, FE, Koo, GC and Schanbacher, BD (1990). Enrichment of bovine X- and Y-chromosome-bearing sperm with monoclonal H-Y antibody-fluorescence-activated cell sorter. Arch Androl 24, 235–45.CrossRefGoogle ScholarPubMed
Almiñana, C, Caballero, I, Heath, PR, Maleki-Dizaji, S, Parrilla, I, Cuello, C, Gil, MA, Vazquez, JL, Vazquez, JM, Roca, J, Martinez, EA, Holt, WV and Fazeli, A (2014). The battle of the sexes starts in the oviduct: modulation of oviductal transcriptome by X and Y-bearing spermatozoa. BMC Genom 15, 293.CrossRefGoogle ScholarPubMed
Asma-Ul-Husna Awan, MA, Mehmood, A, Sultana, T, Shahzad, Q, Ansari, MS, Rakha, BA, Saqlan Naqvi, SM and Akhter, S (2017). Sperm sexing in Nili-Ravi buffalo through modified swim up: validation using SYBR® green real-time PCR. Anim Reprod Sci 182, 69–76.CrossRefGoogle Scholar
Benet, J, Genescà, A, Navarro, J, Egozcue, J and Templado, C (1992). Cytogenetic studies in motile sperm from normal men. Hum Genet 89, 176–80.CrossRefGoogle ScholarPubMed
Bergstrom, DE, Young, M, Albrecht, KH and Eicher, EM (2000). Related function of mouse SOX3, SOX9, and SRY HMG domains assayed by male sex determination. Genesis 28, 111–24.3.0.CO;2-5>CrossRefGoogle ScholarPubMed
Bermejo-Alvarez, P, Rizos, D, Rath, D, Lonergan, P and Gutierrez-Adan, A (2010). Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts. Proc Natl Acad Sci USA 107, 3394–9.CrossRefGoogle ScholarPubMed
Boettcher, M and McManus, MT (2015). Choosing the right tool for the job: RNAi, TALEN, or CRISPR. Mol Cell 58, 575–85.CrossRefGoogle ScholarPubMed
Carvalho, JO, Silva, LP, Sartori, R and Dode, MA (2013). Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy. PLoS One 8, e59387.CrossRefGoogle ScholarPubMed
Chandler, JE, Steinholt-Chenevert, HC, Adkinson, RW and Moser, EB (1998). Sex ratio variation between ejaculates within sire evaluated by polymerase chain reaction, calving, and farrowing records. J Dairy Sci 81, 1855–67.CrossRefGoogle ScholarPubMed
Chen, X, Yue, Y, He, Y, Zhu, H, Hao, H, Zhao, X, Qin, T and Wang, D (2014). Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray. Mol Reprod Dev 81, 908–17.CrossRefGoogle Scholar
Chen, X, Zhu, H, Wu, C, Han, W, Hao, H, Zhao, X, Du, W, Qin, T, Liu, Y and Wang, D (2012). Identification of differentially expressed proteins between bull X and Y spermatozoa. J Proteom 77, 59–67.CrossRefGoogle ScholarPubMed
Cocquet, J, Ellis, PJ, Mahadevaiah, SK, Affara, NA, Vaiman, D and Burgoyne, PS (2012). A genetic basis for a postmeiotic X versus Y chromosome intragenomic conflict in the mouse. PLoS Genet 8, e1002900.CrossRefGoogle ScholarPubMed
Cui, KH (1997). Size differences between human X and Y spermatozoa and prefertilization diagnosis. Mol Hum Reprod 3, 61–67.CrossRefGoogle ScholarPubMed
Cui, KH and Matthews, CD (1993). X larger than Y. Nature 366, 117–8.CrossRefGoogle ScholarPubMed
Douglas, C and Turner, JMA (2020). Advances and challenges in genetic technologies to produce single-sex litters. PLoS Genet 16, e1008898.CrossRefGoogle ScholarPubMed
Gadêlha, H, Hernández-Herrera, P, Montoya, F, Darszon, A and Corkidi, G (2020). Human sperm uses asymmetric and anisotropic flagellar controls to regulate swimming symmetry and cell steering. Sci Adv 6, eaba5168.CrossRefGoogle ScholarPubMed
Geraedts, JP (1997). X spermatozoa larger than Y in 1973. Mol Hum Reprod 3, 545–546.CrossRefGoogle Scholar
Hashimoto, H, Eto, T, Suemizu, H and Ito, M (2013). Application of a new convenience gender sorting method for mouse spermatozoa to mouse reproductive engineering technology. J Vet Med Sci 75, 231–5.CrossRefGoogle ScholarPubMed
Hazzouri, M, Rousseaux, S, Mongelard, F, Usson, Y and Sèle, B (2015). Genome organization in the human sperm nucleus studied by FISH and confocal microscopy. Mol Reprod Dev 55, 307–15.3.0.CO;2-P>CrossRefGoogle Scholar
He, Q, Wu, S, Huang, M, Wang, Y, Zhang, K, Kang, J, Zhang, Y and Quan, F (2021). Effects of diluent pH on enrichment and performance of dairy goat X/Y sperm. Front Cell Dev Biol 9, 747722.CrossRefGoogle ScholarPubMed
Heidari, M, Lakpour, N, Darbandi, M, Darbani, S, Shani, S, Goharbakhsh, L, Cheshmi, G, Akhondi, MM and Sadeghi, MR (2018). Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity. Int J Reprod Biomed 16, 463–8.Google ScholarPubMed
Hogarth, C, Itman, C, Jans, DA and Loveland, KL (2005). Regulated nucleocytoplasmic transport in spermatogenesis: a driver of cellular differentiation? bioEssays 27, 1011–25.CrossRefGoogle ScholarPubMed
Hook, KA and Fisher, HS (2020). Methodological considerations for examining the relationship between sperm morphology and motility. Mol Reprod Dev 87, 633–49.CrossRefGoogle ScholarPubMed
Hossain, AM, Barik, S and Kulkarni, PM (2001). Lack of significant morphological differences between human X and Y spermatozoa and their precursor cells (spermatids) exposed to different prehybridization treatments. J Androl 22, 119–23.CrossRefGoogle ScholarPubMed
Ishijima, SA, Okuno, M, Odagiri, H, Mohri, T and Mohri, H (1992). Separation of X- and Y-chromosome-bearing murine sperm by free-flow electrophoresis: evaluation of separation using PCR. Zool Sci 9, 601–6.Google ScholarPubMed
Johnson, LA, Flook, JP and Hawk, HW (1989). Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 41, 199–203.CrossRefGoogle ScholarPubMed
Johnson, LA, Welch, GR, Keyvanfar, K, Dorfmann, A, Fugger, EF and Schulman, JD (1993). Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 8, 1733–9.CrossRefGoogle ScholarPubMed
Kato, T, Miyata, K, Sonobe, M, Yamashita, S, Tamano, M, Miura, K, Kanai, Y, Miyamoto, S, Sakuma, T, Yamamoto, T, Inui, M, Kikusui, T, Asahara, H and Takada, S (2013). Production of Sry knockout mouse using TALEN via oocyte injection. Sci Rep 3, 3136.CrossRefGoogle ScholarPubMed
Koopman, P, Gubbay, J, Vivian, N, Goodfellow, P and Lovell-Badge, R (1991). Male development of chromosomally female mice transgenic for Sry. Nature 351, 117–121.CrossRefGoogle ScholarPubMed
Kurtz, S, Lucas-Hahn, A, Schlegelberger, B, Göhring, G, Niemann, H, Mettenleiter, TC and Petersen, B (2021). Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs. Proc Natl Acad Sci USA 118, e2008743118.CrossRefGoogle ScholarPubMed
Kurtz, S and Petersen, B (2019). Pre-Determination of sex in pigs by application of CRISPR/Cas system for genome editing. Theriogenology 137, 67–74.CrossRefGoogle ScholarPubMed
Kwon, WS, Rahman, MS, Lee, JS, Kim, J, Yoon, SJ, Park, YJ, You, YA, Hwang, S and Pang, MG (2014). A comprehensive proteomic approach to identifying capacitation related proteins in boar spermatozoa. BMC Genom 15, 897.CrossRefGoogle ScholarPubMed
Lepine, S, McDowell, S, Searle, LM, Kroon, B, Glujovsky, D and Yazdani, A (2019). Advanced sperm selection techniques for assisted reproduction. Cochrane Database Syst Rev 7, CD010461.Google ScholarPubMed
Li, L, Mao, B, Wu, S, Lian, Q, Ge, RS, Silvestrini, B and Cheng, CY (2018). Regulation of spermatid polarity by the actin- and microtubule (MT)-based cytoskeletons. Semin Cell Dev Biol 81, 88–96.CrossRefGoogle ScholarPubMed
Lobel, SM, Pomponio, RJ and Mutter, GL (1993). The sex ratio of normal and manipulated human sperm quantitated by the polymerase chain reaction. Fertil Steril 59, 387–92.CrossRefGoogle ScholarPubMed
Madrid-Bury, N, Fernández, R, Jiménez, A, Pérez-Garnelo, S, Moreira, PN, Pintado, B, de la Fuente, J and Gutiérrez-Adán, A (2003). Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio. Zygote 11, 229–35.CrossRefGoogle Scholar
Mäkelä, JA, Koskenniemi, JJ, Virtanen, HE and Toppari, J (2019). Testis development. Endocr Rev 40, 857–905.CrossRefGoogle ScholarPubMed
Mitchell, M, Simon, D, Affara, N, Ferguson-Smith, M, Avner, P and Bishop, C (1989). Localization of murine X and autosomal sequences homologous to the human Y located testis-determining region. Genetics 121, 803–9.CrossRefGoogle Scholar
Muehleis, PM and Long, SY (1976). The effects of altering the pH of seminal fluid on the sex ratio of rabbit offspring. Fertil Steril 27, 1438–45.CrossRefGoogle Scholar
Navarro-Costa, PA, Molaro, A, Misra, CS, Meiklejohn, CD and Ellis, PJ (2020). Sex and suicide: the curious case of Toll-like receptors. PLoS Biol 18, e3000663.CrossRefGoogle ScholarPubMed
Ohno, S and Wachtel, SS (1978). On the selective elimination of Y-bearing sperm. Immunogenetics 7, 13–6.CrossRefGoogle ScholarPubMed
Ortega-Ferrusola, C, Gil, MC, Rodríguez-Martínez, H, Anel, L, Peña, FJ and Martín-Muñoz, P (2017). Flow cytometry in spermatology: a bright future ahead. Zuchthygiene 52, 921–31.CrossRefGoogle ScholarPubMed
Oyeyipo, IP, van der Linde, M and du Plessis, SS (2017). Environmental exposure of sperm sex-chromosomes: a gender selection technique. Toxicol Res 33, 315–23.CrossRefGoogle ScholarPubMed
Pratt, NC, Huck, UW and Lisk, RD (1987). Offspring sex ratio in hamsters is correlated with vaginal pH at certain times of mating. Behav Neural Biol 48, 310–6.CrossRefGoogle Scholar
Rahman, MS and Pang, MG (2019). New biological insights on X and Y chromosome-bearing spermatozoa. Front Cell Dev Biol 7, 388.CrossRefGoogle ScholarPubMed
Roldan, ERS and Teves, ME (2020). Understanding sperm physiology: proximate and evolutionary explanations of sperm diversity. Mol Cell Endocrinol 518, 110980.CrossRefGoogle ScholarPubMed
Schmid, M and Steinlein, C (2001). Sex chromosomes, sex-linked genes, and sex determination in the vertebrate class amphibia. EXS 91, 143–76.Google Scholar
Scott, C, de Souza, FF, Aristizabal, VHV, Hethrington, L, Krisp, C, Molloy, M, Baker, MA and Dell’Aqua, JAJ (2018). Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis. Anim Reprod Sci 198, 121–8.CrossRefGoogle ScholarPubMed
Sekido, R and Lovell-Badge, R (2009). Sex determination and SRY: down to a wink and a nudge? Trends Genet 25, 19–29.CrossRefGoogle Scholar
Shettles, LB (1960). Nuclear morphology of human spermatozoa. Obstet Gynecol 16, 10–14.Google ScholarPubMed
Soares, R, Martins, VC, Macedo, R, Cardoso, FA, Martins, SAM, Caetano, DM, Fonseca, PH, Silvério, V, Cardoso, S and Freitas, PP (2019). Go with the flow: advances and trends in magnetic flow cytometry. Anal Bioanal Chem 411, 1839–62.CrossRefGoogle ScholarPubMed
Soh, YQ, Alföldi, J, Pyntikova, T, Brown, LG, Graves, T, Minx, PJ, Fulton, RS, Kremitzki, C, Koutseva, N, Mueller, JL, Rozen, S, Hughes, JF, Owens, E, Womack, JE, Murphy, WJ, Cao, Q, de Jong, P, Warren, WC, Wilson, RK, Skaletsky, H and Page, DC (2014). Sequencing the mouse Y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes. Cell 159, 800–13.CrossRefGoogle ScholarPubMed
Soleymani, B, Mansouri, K, Rastegari-Pouyani, M, Parvaneh, S, Khademi, F, Sharifi Tabar, M and Mostafaie, A (2021). Production of monoclonal antibody against recombinant bovine sex-determining region Y (SRY) and their preferential binding to Y chromosome-bearing sperm. Reprod Domest Anim 56, 270–7.CrossRefGoogle ScholarPubMed
Song, W-H, Mohamed, EA, Pang, W-K, Kang, K-H, Ryu, D-Y, MD Rahman, MS and Pang, M-G (2018). Effect of endocrine disruptors on the ratio of X and Y chromosome-bearing live spermatozoa. Reprod Toxicol 82, 10–17.CrossRefGoogle ScholarPubMed
Song, Y, Liu, T, Wang, Y, Deng, J, Chen, M, Yuan, L, Lu, Y, Xu, Y, Yao, H, Li, Z and Lai, L (2017). Mutation of the Sp1 binding site in the 5′flanking region of SRY causes sex reversal in rabbits. Oncotarget 8, 38176–83.CrossRefGoogle Scholar
Srinivasa Prasad, C, Rangasamy, R and Satheshkumar, S (2010). Sex preselection in domestic animals—current status and future prospects. Vet World 10, 346–348.Google Scholar
Tung, CK, Ardon, F, Fiore, AG, Suarez, SS and Wu, M (2014). Cooperative roles of biological flow and surface topography in guiding sperm migration revealed by a microfluidic model. Lab Chip 14, 1348–56.CrossRefGoogle ScholarPubMed
Umehara, T, Tsujita, N and Shimada, M (2019). Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm. PLoS Biol 17, e3000398.CrossRefGoogle ScholarPubMed
Umehara, T, Tsujita, N, Zhu, Z, Ikedo, M and Shimada, M (2020). A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos. Nat Protoc 15, 2645–67.CrossRefGoogle ScholarPubMed
van Duijn, C (1960). Nuclear structure of human spermatozoa. Nature 188, 916–8.CrossRefGoogle ScholarPubMed
Van Dyk, Q, Mahony, MC and Hodgen, GD (2001). Differential binding of X- and Y-chromosome-bearing human spermatozoa to zona pellucida in vitro andrologia 33, 199–205.CrossRefGoogle Scholar
van Munster, EB, Stap, J, Hoebe, RA, te Meerman, GJ and Aten, JA (1999). Difference in volume of X- and Y-chromosome-bearing bovine sperm heads matches difference in DNA content. Cytometry 35, 125–8.3.0.CO;2-H>CrossRefGoogle ScholarPubMed
Vernet, N, Mahadevaiah, SK, Ellis, PJ, de Rooij, DG and Burgoyne, PS (2012). Spermatid development in XO male mice with varying Y chromosome short-arm gene content: evidence for a Y gene controlling the initiation of sperm morphogenesis. Reproduction 144, 433–45.CrossRefGoogle Scholar
Wallmeier, J, Nielsen, KG, Kuehni, CE, Lucas, JS, Leigh, MW, Zariwala, MA and Omran, H (2020). Motile ciliopathies. Nat Rev Dis Primers 6, 77.CrossRefGoogle ScholarPubMed
Williams, BJ, Ballenger, CA, Malter, HE, Bishop, F, Tucker, M, Zwingman, TA and Hassold, TJ (1993). Non-disjunction in human sperm: results of fluorescence in situ hybridization studies using two and three probes. Hum Mol Genet 2, 1929–36.CrossRefGoogle Scholar
Wu, N, Yu, AB, Zhu, HB and Lin, XK (2012). Effective silencing of Sry gene with RNA interference in developing mouse embryos resulted in feminization of XY gonad. J Biomed Biotechnol 2012, 343891.CrossRefGoogle ScholarPubMed
Xi, JF, Wang, XZ, Zhang, YS, Jia, B, Li, CC, Wang, XH and Ying, RW (2019). Sex control by Zfy siRNA in the dairy cattle. Anim Reprod Sci 200, 1–6.CrossRefGoogle ScholarPubMed
Xie, Y, Xu, Z, Wu, Z and Hong, L (2020). Sex manipulation technologies progress in livestock: a review. Front Vet Sci 7, 481.CrossRefGoogle ScholarPubMed
Yadav, SK, Gangwar, DK, Singh, J, Tikadar, CK, Khanna, VV, Saini, S, Dholpuria, S, Palta, P, Manik, RS, Singh, MK and Singla, SK (2017). An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm. Vet World 10, 498–504.CrossRefGoogle ScholarPubMed
Yang, T and Yang, WX (2020). The dynamics and regulation of microfilament during spermatogenesis. Gene 744, 144635.CrossRefGoogle ScholarPubMed
Yetunde, I and Vasiliki, M (2013). Effects of advanced selection methods on sperm quality and ART outcome. Minerva Ginecol 65, 487–96.Google ScholarPubMed
Yosef, I, Edry-Botzer, L, Globus, R, Shlomovitz, I, Munitz, A, Gerlic, M and Qimron, U (2019). A genetic system for biasing the sex ratio in mice. EMBO Rep 20, e48269.CrossRefGoogle ScholarPubMed
You, YA, Kwon, WS, Saidur Rahman, M, Park, YJ, Kim, YJ and Pang, MG (2017). Sex chromosome-dependent differential viability of human spermatozoa during prolonged incubation. Hum Reprod 32, 1183–91.CrossRefGoogle ScholarPubMed
Zavaczki, Z, Celik-Ozenci, C, Ovari, L, Jakab, A, Sati, GL, Ward, DC and Huszar, G (2006). Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry. Fertil Steril 85, 121–7.CrossRefGoogle Scholar
Zhang, YS, Du, YC, Sun, LR, Wang, XH, Liu, SB, Xi, JF, Li, CC, Ying, RW, Jiang, S, Wang, XZ, Shen, H and Jia, B (2018). A genetic method for sex determination in Ovis spp. by interruption of the zinc finger protein, Y-linked (ZFY) gene on the Y chromosome. Reprod Fertil Dev 30, 1161–8.CrossRefGoogle ScholarPubMed