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Genetics of Resistance to Acetohydroxyacid Synthase Inhibitors in Populations of Eastern Black Nightshade (Solanum ptychanthum) from Ontario

Published online by Cambridge University Press:  20 January 2017

Jamshid Ashigh ,
Istvan Rajcan  and
François J. Tardif
Jamshid Ashigh
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
Istvan Rajcan
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
François J. Tardif*
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada. N1G 2W1
*
Corresponding author's E-mail: [email protected]
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Abstract

Resistance to acetohydroxyacid synthase (AHAS) inhibiting herbicides in populations of eastern black nightshade from Ontario has been linked to an Ala205Val substitution in the AHAS enzyme. The aim of this study was to determine the mechanism of inheritance of AHAS inhibitor resistance and the genetic relationships among resistant (R) and susceptible (S) eastern black nightshade populations from Ontario. Homozygous R and S parental populations were crossed and the inheritance was analyzed in F1 (S × R), reciprocal F1 (R × S), F2, and backcross (S × F1) progenies after application of imazethapyr at 150 g ai ha−1. Compared to parental lines, the progenies were rated as R, intermediate (I), and S phenotypes. All the F1 progenies were of the I phenotype. The backcross progenies segregated in a 1:1 (S:I) ratio, and the F2 families segregated in a 1:2:1 (R:I:S) ratio. These results indicate that a single nuclear gene, with incomplete dominance, controls resistance to AHAS-inhibiting herbicides in R population of eastern black nightshade. Random amplified polymorphic DNA (RAPD) markers were screened among 25 R and S populations. The genetic relationship of R and S populations based on RAPD profiles generated from six RAPD primers indicated four groups of populations in which resistance seems to have arisen independently. However, based on similarity coefficients, resistance within three of the groups could have arisen by gene flow. Both similar local selection pressure and gene flow could explain the spread of the Ala205Val substitution in R populations of eastern black nightshade in Ontario.

Keywords

Imazethapyreastern black nightshade, Solanum ptychanthum Dun. SOLPTAcetolactate synthaseALSRAPDherbicide resistanceinheritancegenetic diversity
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 2 , April 2008 , pp. 210 - 215
Copyright
Copyright © Weed Science Society of America 

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