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Trinigyrus spp. (Monogenea: Dactylogyridae) from Brazilian catfishes: new species, molecular data and new morphological contributions to the genus

Published online by Cambridge University Press:  20 February 2020

L. Franceschini Open the ORCID record for L. Franceschini [Opens in a new window] ,
A.A. Acosta Open the ORCID record for A.A. Acosta [Opens in a new window] ,
A.C. Zago Open the ORCID record for A.C. Zago [Opens in a new window] ,
M.I. Müller Open the ORCID record for M.I. Müller [Opens in a new window]  and
R.J. da Silva Open the ORCID record for R.J. da Silva [Opens in a new window]
L. Franceschini*
Affiliation:
Department of Parasitology, São Paulo State University (UNESP), Institute of Biosciences, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
A.A. Acosta
Affiliation:
Department of Parasitology, São Paulo State University (UNESP), Institute of Biosciences, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil Water Research Group, Unit for Environmental Sciences and Management, Potchefstroom Campus, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa
A.C. Zago
Affiliation:
Department of Parasitology, São Paulo State University (UNESP), Institute of Biosciences, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
M.I. Müller
Affiliation:
Department of Parasitology, São Paulo State University (UNESP), Institute of Biosciences, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil Department of Biological Sciences, Federal University of São Paulo (Unifesp), Rua Professor Arthur Riedel, 275, Jardim Eldorado, Diadema, São Paulo 09972-270, Brazil
R.J. da Silva
Affiliation:
Department of Parasitology, São Paulo State University (UNESP), Institute of Biosciences, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
*
Author for correspondence: L. Franceschini, E-mail: [email protected]
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Abstract

This study describes two new species, Trinigyrus anthus n. sp. and Trinigyrus carvalhoi n. sp., from gills of Hypostomus spp. from the Upper Paraná River basin, Brazil. Trinigyrus peregrinus is redescribed based on examination of its holotype, paratypes and new material of specimens parasitizing Pterygoplichthys ambrosettii, also from the Upper Paraná River basin, Brazil. New morphological features were included in the diagnosis of the genus, such as the presence of a sclerotized border on the anchor base, and a weakly sclerotized fringe on the base of the male copulatory organ (MCO). Trinigyrus anthus n. sp. differs from other congeners by the shape of the MCO, presenting an enlarged base with sclerotized fringes resembling flower petals. Trinigyrus carvalhoi n. sp. and T. peregrinus are similar but can be differentiated from each other mainly by the sclerotization of the vagina (absent in the new species), and the morphology of the MCO (C-shaped versus one counterclockwise circle, respectively). For the first time, gene sequences of Trinigyrus spp. from Brazil were obtained (partial ribosomal 28S and mitochondrial cytochrome c oxidase I (mtCOI)). The genetic divergences among the new species and T. peregrinus varied from 2 to 3% (6‒18 pb) based on sequences of 28S ribosomal DNA (rDNA), and 6–7% (83‒92 pb) using mtCOI. Phylogenetic analyses based on partial 28S rDNA revealed that Trinigyrus, Heteropriapulus and Unilatus formed a monophyletic and well-supported clade of monogeneans from Neotropical freshwater loricariids, suggesting a closer relationship among these dactylogyrids and their hosts.

Keywords

DactylogyridsNeotropical regioncatfishesphylogenyhost–parasite relationships28S rDNAmtCOI
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e126
Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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