Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T00:46:40.905Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of European Pygorchis Looss, 1899

Published online by Cambridge University Press:  26 March 2021

P. Heneberg Open the ORCID record for P. Heneberg [Opens in a new window]  and
J. Sitko
P. Heneberg*
Affiliation:
Third Faculty of Medicine, Charles University, Prague, Czech Republic
J. Sitko
Affiliation:
Comenius Museum, Moravian Ornithological Station, Přerov, Czech Republic
*
Author for correspondence: P. Heneberg, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Adult trematodes of the genus Pygorchis Looss, 1899 (Trematoda: Philophthalmidae) parasitize the cloaca of birds. The genus contains three species, all of which are rarely reported and molecular phylogenetics of which have not been applied. The absence of reference DNA sequences limit studies of their indistinct larval forms. Based on the materials that were obtained from birds of the Czech origin, we performed a molecular characterization of both currently known Pygorchis spp., which are known from the Palearctic, the type species Pygorchis affixus Looss, 1899 and Pygorchis alakolensis Zhatkanbaeva, 1967, and provided morphological description of the examined P. alakolensis specimen. We found that the two species were of similar dimensions; the only difference was in the position of testes and in the extent of vitelline follicles. However, the position of testes in P. affixus was variable, and approximately 10% of examined P. affixus individuals had testes positioned obliquely. The second feature that allows differential diagnostic, the extent of vitelline follicles, was more reproducible as the vitelline follicles of P. affixus did not extend beyond the intestinal caeca, or, in exceptional cases, they extended them at only one side. In the examined P. alakolensis individual, the testes were positioned obliquely, and the vitelline follicles extended beyond the intestinal caeca. We reported P. alakolensis for the first time from Europe; previously, it was known only from Central Asian lakes and rivers. We confirmed the classification of Pygorchis into Philophtalmidae.

Keywords

Cloacacommon terngrey heronPhilophthalmidaePygorchis affixusPygorchis alakolensis
Type
Short Communication
Information
Journal of Helminthology , Volume 95 , 2021 , e18
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bowles, J, Blair, D and McManus, DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–173.CrossRefGoogle ScholarPubMed
Bray, RA, Littlewood, DTJ, Herniov, EA, Williams, B and Henderson, RE (1999) Digenean parasites of deep-sea teleosts: a review and case studies of intrageneric phylogenies. Parasitology 119, 5125–5144.Google ScholarPubMed
Bykhovskaya-Pavlovskaya, IE (1974) Trematodes of birds in Kursk barrier beach. Parasitologicheskiy Sbornik 26, 39–80 (in Russian).Google Scholar
Dronen, NO (1985) Digenetic trematodes from the roseate spoonbill, Ajaja ajaja, from the Texas Gulf Coast. Transactions of the American Microscopical Society 104, 261–266.CrossRefGoogle Scholar
Heneberg, P, Sitko, J, Těšínský, M, Rząd, I and Bizos, J (2018) Central European Strigeidae Railliet, 1919 (Trematoda: Strigeidida): molecular and comparative morphological analysis suggests the reclassification of Parastrigea robusta Szidat, 1928 into Strigea Abildgaard, 1790. Parasitology International 67, 688–701.CrossRefGoogle ScholarPubMed
Iskova, NI, Sharpilo, VP, Sharpilo, L-D and Tkach, VV (1995) Catalogue of helminths of Ukraine. Trematodes of terrestrial vertebrates. Kiev, National Academy of Sciences of Ukraine (in Russian).Google Scholar
Ivanov, VM, Semenova, NN, Parshina, OY, Kalmykov, AP and Fedorovich, VV (2003) Monitoring, structural changes and ecological features of the trematodo fauna of vertebrates in the Volga delta and the North Caspian. Astrakhan, Astrakhan State University (in Russian).Google Scholar
Kralj, J, Martinović, M, Jurinović, L, Szinai, P, Sütő, S and Preiszner, B (2020) Geolocator study reveals east African migration route of Central European Common Terns. Avian Research 11, 6.CrossRefGoogle Scholar
Loos, A (1899) Weitere Beiträge zur Kenntnis der Trematodenfauna Aegyptens zugleich versuch einer naturlichen Gliederung des Genus Distomum Retzius. Zoologische Jahrbücher. Abteilung für Systematik, Geographie und Biologie der Tiere 12, 521–784.Google Scholar
Morgan, JA and Blair, D (1998) Mitochondrial ND1 gene sequences used to identify Echinostome isolates from Australia and New Zealand. International Journal for Parasitology 28, 493–502.CrossRefGoogle ScholarPubMed
Nekrasov, AV, Pronin, NM, Sanzhieva, SD and Timoshenko, TM (1999) Diversity of helminth fauna in the herring gull (Larus argentatus) from the Baikal lake: peculiarities of spatial distribution and invasion. Parazitologiya 33, 426–436 (in Russian).Google Scholar
Routtu, J, Grunberg, D, Izhar, R, Dagan, Y, Guttel, Y, Ucko, M and Ben-Ami, F (2014) Selective and universal primers for trematode barcoding in freshwater snails. Parasitology Research 113, 2535–2540.CrossRefGoogle ScholarPubMed
Schuster, RK (2013) A new species of Cloacitrema (Digenea, Philophthalmidae) from greater flamingo (Phoenicopterus roseus) with remarks on the genera Cloacitrema and Pygorchis. Vestnik Zoologii 47, 83–87.CrossRefGoogle Scholar
Semenova, NN (1983) Trematode fauna of Laridae in the Volga Delta and northern Caspian Sea areas. Parazitologicheskie issledovaniya v zapovednikakh Sbornik Nauchnykh Trudov Tsentral’ noi Laboratorii Okhotnich’ ego Khozyaistva i Zapovednikov 22, 23–39 (in Russian).Google Scholar
Semenova, NN and Ivanov, VM (1985) The rare trematode species Pygorchis alacolensis in the northern part of the Caspian Sea. Parazitologiya 19, 324–326 (in Russian).Google Scholar
Sitko, J (1999) Trematodes of herons and ibises (Ciconiiformes) in Czech Republic. Helminthologia 36, 130–131.Google Scholar
Sitko, J (2012) Trematodes of herons (Aves: Ciconiiformes) in the Czech Republic. Helminthologia 49, 33–42.CrossRefGoogle Scholar
Sitko, J and Heneberg, P (2015) Composition, structure and pattern of helminth assemblages associated with central European herons (Ardeidae). Parasitology International 64, 100–112.CrossRefGoogle Scholar
Sitko, J, Faltýnková, A and Scholz, T (2006) Checklist of the trematodes (Digenea) of birds of the Czech and Slovak Republics. Prague, Academia.Google Scholar
Sitko, J, Bizos, J and Heneberg, P (2017) Central European parasitic flatworms of the Cyclocoelidae Stossich, 1902 (Trematoda: Plagiorchiida): molecular and comparative morphological analysis suggests the reclassification of Cyclocoelum obscurum (Leidy, 1887) into the Harrahium Witenberg, 1926. Parasitology 144, 368–383.CrossRefGoogle ScholarPubMed
Zhatkanbaeva, D (1967) Pygorchis alakolensis sp. nov. – a new trematode found in fisheating birds of Kazakhstan. Izvestiya Akademii Nauk Kazakhskoi SSR Seriya Biologicheskikh Nauk 5, 60–62 (in Russian).Google Scholar