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Evaluation of the Baermann–Wetzel method for detecting lungworm larvae in wild ruminants from faecal samples

Published online by Cambridge University Press:  08 March 2021

T. Carrau Open the ORCID record for T. Carrau [Opens in a new window] ,
C. Martínez-Carrasco Open the ORCID record for C. Martínez-Carrasco [Opens in a new window] ,
M.M. Garijo Open the ORCID record for M.M. Garijo [Opens in a new window] ,
F. Alonso Open the ORCID record for F. Alonso [Opens in a new window] ,
R. Ruiz de Ybáñez Open the ORCID record for R. Ruiz de Ybáñez [Opens in a new window]  and
P. Tizzani Open the ORCID record for P. Tizzani [Opens in a new window]
T. Carrau
Affiliation:
Parasitología, Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad de Murcia, 30100 Espinardo, Murcia, Spain
C. Martínez-Carrasco
Affiliation:
Parasitología, Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad de Murcia, 30100 Espinardo, Murcia, Spain
M.M. Garijo
Affiliation:
Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/ Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, Spain
F. Alonso
Affiliation:
Parasitología, Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad de Murcia, 30100 Espinardo, Murcia, Spain
R. Ruiz de Ybáñez*
Affiliation:
Parasitología, Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad de Murcia, 30100 Espinardo, Murcia, Spain
P. Tizzani
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2–10095 Grugliasco, Torino, Italy
*
Author for correspondence: R. Ruiz de Ybañez E-mail: [email protected]
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Abstract

Lungworms can exert a negative impact on wild ruminant fitness; for this reason, the diagnosis of the associated diseases is an important prevention measure. The Baerman–Wetzel technique is the most usual method for the diagnosis of bronchopulmonary nematodes and is based on the active migration and movement of their first-stage larvae. Pulmonary tissue samples are frequently used for the post-mortem diagnosis of these parasites, but this kind of sample is not always available and easy to obtain. Faecal samples represent a more accessible choice for parasite monitoring. This work aimed to evaluate the agreement between the results obtained by the Baermann–Wetzel technique when samples of lung parenchyma or faeces from wild ruminants are used. A good level of agreement as well as a similar sensitivity between the two types of sample were observed, validating the use of faecal samples as a less invasive and cost-effective alternative for the monitoring of lungworm in wild ruminant populations.

Keywords

Baermann–Wetzel techniqueBland–Altmanndiagnosticlungwormswild ruminant
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e13
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Alasaad, S, Morrondo, P, Dacal-Rivas, V, Soriguer, RC, Granados, JE, Serrano, E, Zhu, XQ, Rossi, L and Pérez, JM (2009) Bronchopulmonary nematode infection of Capra pyrenaica in the Sierra Nevada massif, Spain. Veterinary Parasitology 164, 340–343.CrossRefGoogle ScholarPubMed
Andermatt-Mettler, I, Eckert, J, Ramp, T and Gottstein, B (1987) Cryopreservation of Dictyocaulus viviparus third-stage larvae and Trichinella spiralis muscle larvae. Parasitology Research 73, 358–365.CrossRefGoogle ScholarPubMed
Berrag, B and Urquhart, GM (1996) Epidemiological aspects of lungworm infections of goats in Morocco. Veterinary Parasitology 61, 81–95.CrossRefGoogle ScholarPubMed
Bienioschek, S, Rehbein, S and Ribbeck, R (1996) Cross-infections between fallow deer and domestic ruminants with large lungworms (Dictyocaulus spp.). Applied Parasitology 37(4), 229–238.Google ScholarPubMed
Bland, M and Altman, DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. The Lancet 1(8476), 307–310.CrossRefGoogle ScholarPubMed
Carreno, RA and Hoberg, EP (1999) Evolutionary relationships among the protostrongylidae (Nematoda: Metastrongyloidea) as inferred from morphological characters, with consideration of parasite-host coevolution. The Journal of Parasitology 85, 638–648.CrossRefGoogle ScholarPubMed
Cassini, R, Párraga, MA, Signorini, M, Frangipane di Regalbono, A, Sturaro, E, Rossi, L and Ramanzin, M (2015) Lungworms in alpine ibex (Capra ibex) in the eastern Alps, Italy: an ecological approach. Veterinary Parasitology 214, 132–138.CrossRefGoogle Scholar
Díez-Baños, MN and Hidalgo-Argüello, M (2006) Análisis de la estado parasitario de rumiantes silvestres en el norte de Castilla León. pp. 95–102 in Díez Baños P, Benedito Castellote JL, Patrocinio Morrondo Pelayo M, Hernández Bermúdez J and López Sández CM (Eds) Veinte Años de Buiatría: Actas Del XIV Congreso Internacional de La Federación Mediterránea de Sanidad y Producción de Rumiantes, Lugo-Santiago de Compostela, 12–15 July 2006.Google Scholar
Diez-Baños, P, Diez-Baños, N, Morrondo-Pelayo, MP and Campillo, MCD (1990) Broncho-pulmonary helminths of chamois (Rupicapra rupicapra parva) captured in north-west Spain: assessment from first stage larvae in faeces and lungs. Annales de Parasitologie Humaine et Comparee 65, 74–79.CrossRefGoogle ScholarPubMed
Eysker, M (1997) The sensitivity of the Baermann method for the diagnosis of primary Dictyocaulus viviparus infections in calves. Veterinary Parasitology 69, 89–93.CrossRefGoogle Scholar
Forrester, SG and Lankester, MW (1997) Extracting protostrongylid nematode larvae from ungulate feces. Journal of Wildlife Diseases 33, 511–516.CrossRefGoogle ScholarPubMed
Gunn, A and Irvine, RJ (2003) Subclinical parasitism and ruminant foraging strategies: a review. Wildlife Society Bulletin 1973-2006 31, 117–126.Google Scholar
Jenkins, MC (2001) Advances and prospects for subunit vaccines against protozoa of veterinary importance. Veterinary Parasitology. Advances in Molecular Parasitology 101, 291–310.CrossRefGoogle ScholarPubMed
Kafle, P, Lejeune, M, Verocai, GG, Hoberg, EP and Kutz, SJ (2015) Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic. International Journal for Parasitology: Parasites and Wildlife 4, 283–290.Google Scholar
Kelly, DW, Paterson, RA, Townsend, CR, Poulin, R and Tompkins, DM (2009) Parasite spillback: a neglected concept in invasion ecology? Ecology 90, 2047–2056.CrossRefGoogle ScholarPubMed
Lavín, S, Marco, I, Rossi, L, Meneguz, PG and Viñas, L (1997) Haemonchosis in Spanish ibex. Journal of Wildlife Diseases 33, 656–659.CrossRefGoogle ScholarPubMed
Levine, ND (1985) Veterinary protozoology. Ames, Iowa State University Press.Google Scholar
MAFF (1986) Manual of veterinary parasitological laboratory techniques. London, Her Majesty's Stationery Office.Google Scholar
Mansfield, LS, Gamble, HR, Baker, JS and Lichtenfels, JR (1993) Lungworm infection in a sheep flock in Maryland. Journal of the American Veterinary Medical Association 202(4), 601–606.Google Scholar
Marreros, N, Frey, CF, Willisch, CS, Signer, C and Ryser-Degiorgis, MP (2012) Coprological analyses on apparently healthy alpine ibex (Capra ibex ibex) from two Swiss colonies. Veterinary Parasitology 186, 382–389.CrossRefGoogle ScholarPubMed
Meana, A, Luzón-Peña, M, Santiago-Moreno, J, De Bulnes, A and Gómez-Bautista, M (1996) Natural infection by gastrointestinal and bronchopulmonary nematodes in mouflons (Ovis musimon) and their response to netobimin treatment. Journal of Wildlife Diseases 32(1), 39–43.CrossRefGoogle ScholarPubMed
Nocture, M, Cabaret, J and Hugonnet-Chapelle, L (1998) Protostrongylid nematode infection of chamois (Rupicapra rupicapra) at the Bauges massif (French Alps). Veterinary Parasitology 77, 153–161.CrossRefGoogle Scholar
Panadero, R, Carrillo, EB, López, C, Dı́ez-Baños, N, Dı́ez-Baños, P and Morrondo, MP (2001) Bronchopulmonary helminths of roe deer (Capreolus capreolus) in the northwest of Spain. Veterinary Parasitology 99, 221–229.CrossRefGoogle Scholar
Panuska, C (2006) Lungworms of ruminants. The Veterinary Clinics of North America. Small Animal Practice 22, 583–593.CrossRefGoogle ScholarPubMed
Putman, RJ (1984) Facts from faeces. Mammal Review 14, 79–97.CrossRefGoogle Scholar
Pyziel, AM (2014) Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU). Acta Parasitologica 59, 122–125.CrossRefGoogle Scholar
Rode, B and Jørgensen, RJ (1989) Baermannization of Dictyocaulus spp. from faeces of cattle, sheep and donkeys. Veterinary Parasitology 30, 205–211.CrossRefGoogle ScholarPubMed
Rose, JH (1957) Observations on the bionomics of the free-living first stage larvae of the sheep lungworm, Muellerius capillaris. Journal of Helminthology 31, 17–28.CrossRefGoogle Scholar
RStudio Team (2015) Studio: integrated development for R. Boston, MA, RStudio, Inc.Google Scholar
Sleeman, J, Richgels, K, White, C and Stephen, C (2019) Integration of wildlife and environmental health into a one health approach. Revue Scientifique et Technique 38, 91–102.CrossRefGoogle ScholarPubMed
Traversa, D, Iorio, R and Otranto, D (2008) Diagnostic and clinical implications of a nested PCR specific for ribosomal DNA of the feline lungworm Aelurostrongylus abstrusus (Nematoda, Strongylida). Journal of Clinical Microbiology 46, 1811–1817.CrossRefGoogle Scholar
van Wyk, JA, Cabaret, J and Michael, LM (2004) Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology 119, 277–306.CrossRefGoogle ScholarPubMed
Vicente, J, Fierro, Y and Gortázar, C (2005) Seasonal dynamics of the fecal excretion of Elaphostrongylus cervi (Nematoda, metastrongyloidea) first-stage larvae in Iberian red deer (Cervus elaphus hispanicus) from southern Spain. Parasitology Research 95, 60–64.CrossRefGoogle Scholar
Viña, M, Panadero, R, Díaz, P, Fernández, G, Pérez, A, Díez-Baños, P, Morrondo, P and López, CM (2013) Evaluation of the use of pooled fecal samples for the diagnosis of protostrongylid infections in sheep. Veterinary Parasitology 197, 231–234.CrossRefGoogle Scholar
Willard, MD, Roberts, RE, Allison, N, Grieve, RB and Escher, K (1988) Diagnosis of Aelurostrongylus abstrusus and Dirofilaria immitis infections in cats from a human shelter. Journal of the American Veterinary Medical Association 192, 913–916.Google ScholarPubMed