Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-28T14:24:43.378Z Has data issue: false hasContentIssue false
  • English
  • Français

Some studies on pathogenicity of Coelomomyces stegomyiae against mosquito larvae in the laboratory

Published online by Cambridge University Press:  19 September 2011

E. S. Nnakumusana
E. S. Nnakumusana
Affiliation:
Department of Entomology, Uganda Virus Research Institute, P.O. Box 49, Entebbe, Uganda
Get access

Abstract

Studies were conducted with Ugandan isolate of Coelomomyces stegomyiae against mosquito larvae. Aedes aegypti first instar larvae was most susceptible (99.8%) within 6 days, and the fourth instar was least susceptible (44.4%). Culex quinquefasciatus suffered 70% mortality within 10 days. Mortality rates were 92.5% for Culex tigripes, 70% for Culex duttoni, 89.5% for Aedes africanus, 89.9% for Anopheles gambiase and 97.3% for Aedes simpsoni. There was no infection when two species of the predatory mosquito Toxorhynchites were exposed to infection. A few adults emerging from an infected larval population were found infected when dissected. Other mosquitoes that pupated died and the pupae were found infected. Infected females were produced by 19.2% of the fresh pupae exposed. The importance of the results in biological control is discussed.

Résumé

Des études ont été faites sur le Coelomomyces stegomyiae isolé de l'Uganda. Celui-ci a été utilisé contre les larves de moustique. Les larves d'Aedes aegypti au premier stade étaient les plus susceptibles (99,8%) dans 6 jours et les larves ayant atteint le 4ème stade étaient les moins susceptibles (44,4%). Culex quinquefasciatus a subi une mortalité de 70% dans 10 jours. Le taux de mortalité de Culex tigripes était de 92,5%, le taux de mortalité de Culex duttoni était de 70%, celui de Aedes africanus 89,5%. Le taux de mortalité d'Anopheles gambiae était de 89,9%. Celui de Aedes simpsoni était de 97,3%. Il n'y a pas eu d'infection lorsque deux espèces de moustique prédatrice Toxorhynchites étaient exposées. Quelques adultes qui avaient émergé d'une population larvaire infectée ont montré des signes d'infection lors de la dissection. D'autres moustiques au stade de nymphe sont mortes et on a trouvé que les nymphes étaient infectés. 19,2% Des nouveaux nymphes exposés ont produit des femelles infectées. L'importance des résultats des le contrôle biologique est discutée.

Keywords

Coelomomyces stegomyiaeAnopheles gambiaeCulex quinquefasciatusCulex tigripesAedes aegyptiAedes africanusCulex duttonibiological controlAedes simpsoniCoelomomyces stegomyaieAnopheles gambiaeCulex quinquefasciatusCulex trigripesAedes aegyptiAedes africanusCulex duttonicontrôle biologiqueAedes simpsoni
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 7 , Issue 1 , February 1986 , pp. 93 - 97
Copyright
Copyright © ICIPE 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bland, C. E., Couch, J. N. and Newell, S. Y. (1980) Identification of Coelomomyces Saprolegniates and Lagenidiales. In Microbial Control of Vectors and Pests, 1970–1980 Edition (Edited by Burgess, H. D.), pp. 129–162. Academic Press, London.Google Scholar
Brady, J. (1963) Results of age-grouping dissections on four species of Anopheles from southern Ghana. Bull. Wld Hlth Org. 29, 147 153.Google ScholarPubMed
Castillo, J. M. and Roberts, D. W. (1980) Coelomomyces pathogens of Culicidae (Mosquitoes) In Supplement to Vol. 58 of Bulletin of the WHO Bibliography on Pathogens of Medically Important Arthropods, pp. 53–67.Google Scholar
Chamberlain, R. W. and Sudia, W. D. (1961) Mechanisms of transmission of viruses by mosquitoes. A. Rev. Ent. 6, 371–390.CrossRefGoogle ScholarPubMed
Federici, B. A. (1980) Production of the mosquito–parasitic fungus Coelomomyces dodgei, through synchronized infection and growth of the intermediate copepod host, Cyclops vernalis. Entomophaga 25, 209–217.CrossRefGoogle Scholar
Federici, B. A. and Roberts, D. W. (1976) Infection of mosquito larvae with fungi of the genus Coelomomyces 11. Experiments with Coelomomyces punctatus in Anopheles quadrimaculatus. J. invertebr. Path. 27, 333–341.CrossRefGoogle Scholar
Federici, B. A. and Chapman, H. C. (1977) Coelomomyces dodgei: Establishment of an in-vivo laboratory culture. J. invertebr. Path. 30, 288–297.CrossRefGoogle ScholarPubMed
Haddow, A. J. (1948) The mosquitoes of Bwamba county Uganda VI. Mosquito breeding in plant axils. Bull. ent. Res. 39, 185–212.CrossRefGoogle Scholar
Hall, R. A. and Papierok, B. (1982) Fungi as biological control agents of arthropods of agricultural and medical importance. Parasitology 84, 205–240.CrossRefGoogle Scholar
Hellen, W. R., Clark, T. B. and Lindergren, J. E. (1963) A host record of Coelomomyces psonophorae Couch in California. J. invertebr. Path. 5, 167–173.Google Scholar
McClelland, G. A. H. (1960) Observations on the mosquito Aedes (stegomyiae) aegypti (L) in East Africa. 11. The biting cycle in a domestic population at the Kenya coast. Bull. ent. Res. 50, 687–696.CrossRefGoogle Scholar
McCrae, A. W. R. (1972) Age composition of man biting Ae. (stegomyiae) simpsoni (Theobald) (Diptera: Culicidae) in Bwamba county, Uganda. J. med. Ent. 9, 545–550.CrossRefGoogle Scholar
Mukwaya, L. G. (1977) Genetic control of feeding preference in the mosquito Aedes (stegomyiae) simpsoni and aegypti. Physiol. Ent. 22, 133–145.CrossRefGoogle Scholar
Nnakumusana, E. S. (1986) The effect of Coelomomyces indicus on the fecundity and longevity of Anopheles gambiae, Culex fatigans and Aedes aegypti exposed to infection at each larval instar. Insect Sci. Applic. In press.CrossRefGoogle Scholar
Taylor, B. W., Harlos, J. A. and Brust, A. R. (1980) Coelomomyces infection of adult female mosquito Aedes trivittatus (coquillett) in Manitoba. Can. J. Zool. 58, 1215–1219.CrossRefGoogle Scholar
Teesdale, C. (1955) Studies on the bionomics of Aedes aegypti (L) in its natural habitats in a coast region of Kenya. Bull. ent. Res. 46, 711–741.CrossRefGoogle Scholar
Undeen, A. H. and Nolan, R. A. (1977) Ovarian infection and fungal spore oviposition in the black fly Prosimulium mixtum. J. invertebr. Path. 30, 97–98.CrossRefGoogle Scholar
Undeen, A. H. (1978) Observations on the ovarian phycomycete of Newfoundland black flies. Proc. Int. Coll. Invetebr. Path, and Xlth Ann. Mecr. Soc. Invertebr. Path. Prague Czechoslovakia, Sept. 11–17.Google Scholar
Walker, A. J. (1938) Fungal infection of mosquitoes especially of Anopheles costalia. Ann. trop. Med. Parasit. 32, 231–244.CrossRefGoogle Scholar
Whisler, H. C., Zebold, S. L. and Shemanchuk, J. A. (1975) Life history of Coelomomyces psorophorae. Proc. natn. Acad. Sci. U.S.A. 72, 963–966.CrossRefGoogle ScholarPubMed
Wood, R. J. (1962) Oviposition in D.D.T. resistant and susceptible strains of Aedes aegypti (L) egg laying on open water surfaces. Bull. ent. Res. 52, 785–790.Google Scholar