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Growth performance and immune responses in chickens after challenge with lipopolysaccharide and modulation by dietary different oils

Published online by Cambridge University Press:  01 February 2008

X. Yang ,
Y. Guo ,
X. He ,
J. Yuan ,
Y. Yang  and
Z. Wang
X. Yang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China College of Animal Science and Technology, Northwest A&F University, Yangling 712100, People’s Republic of China
Y. Guo*
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
X. He
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
J. Yuan
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
Y. Yang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
Z. Wang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
*
E-mail: [email protected]
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Abstract

The study was conducted to investigate the effects of different oils on growth performance and immune responses of chickens after challenge with lipopolysaccharide (LPS). A total of 288 chickens were assigned in a 2 × 2 factorial design. Factors were dietary fat type (4.5% maize oil or 4.5% fish oil) and immunological challenge (LPS or saline). At 20 days and 27 days of age, chickens were injected intraperitoneally with either 1 mg/kg body weight of LPS or sterile saline. LPS decreased feed intake from 21 days to 28 days of age and body-weight gain from 21 days to 42 days of age. Fish oil improved feed-conversion efficiency of chickens after LPS challenge for the first time. Fish oil supplementation decreased lymphocyte proliferation (21 days: P < 0.0001; 28 days: P < 0.0001) and the ratio of CD3+CD4+/CD3+CD8+ (21 days: P = 0.0479; 28 days: P = 0.0009) after LPS challenge. LPS challenge increased the levels of interleukin-1 (IL-1) (21 days: P < 0.0001; 28 days: P = 0.0030), IL-6 (21 days: P < 0.0001; 28 days: P = 0.0001) and tumor necrosis factor-α (TNF-α) (21 days: P = 0.0008; 28 days: P = 0.0018). And fish oil alleviated the elevations in the production of IL-6 (21 days: P = 0.0359; 28 days: P = 0.0302) and TNF-α (21 days: P = 0.0055; 28 days: P = 0.0391) induced by the LPS challenge. Fish oil alleviated the mRNA abundance elevation of nuclear factor kappa B (NFκB) (21 days: P = 0.0079; 28 days: P = 0.0017) after LPS challenge. These results showed that fish oil acts as an anti-inflammatory agent, which may be associated with down-regulation of the activated immune system. The results of pro-inflammatory cytokines and mRNA abundance results suggested that fish oil might alleviate the elevation of IL-6 and TNF-α induced by LPS through down-regulating NFκB expression.

Keywords

chickensfish oilimmune responsesmaize oilpro-inflammatory cytokines
Type
Full Paper
Information
animal , Volume 2 , Issue 2 , February 2008 , pp. 216 - 223
Copyright
Copyright © The Animal Consortium 2008

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References

Balaji, R, Wright, KJ, Turner, JL, Hill, CM, Dritz, SS, Fenwick, B, Carroll, JA, Zannelli, ME, Beausang, LAMinton, JE 2002. Circulating cortisol, tumor necrosis factor-α interleukin-1β, and interferon-γ in pigs infected with Actinobacillus pleuropneumoniae. Journal of Animal Science 80, 202–207.CrossRefGoogle ScholarPubMed
Benson, BN, Calvert, CC, Roura, EKlasing, KC 1993. Dietary energy source and density modulate the expression of immunologic stress in chicks. Journal of Nutrition 123, 1714–1723.CrossRefGoogle ScholarPubMed
Calder, PC 2001a. N-3 polyunsaturated fatty acids, inflammation and immunity: pouring oil on troubled waters or another fishy tale? Nutrition Research 21, 309–341.CrossRefGoogle Scholar
Calder, PC 2001b. The ratio of n-6 to n-3 fatty acids in the diet: impact on T lymphocyte function. European Journal of Lipid Science and Technology 103, 390–398.3.0.CO;2-3>CrossRefGoogle Scholar
Calder, PCGrimble, RF 2002. Polyunsaturated fatty acids, inflammation and immunity. European Journal of Clinical Nutrition 56, s14–s19.CrossRefGoogle ScholarPubMed
Cook, ME, Miller, CC, Park, YPariza, M 1993. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Science 72, 1301–1305.CrossRefGoogle ScholarPubMed
Gaines, AM, Carroll, JA, Yi, GF, Allee, GLZannelli, ME 2003. Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs: II. Effects on the immune axis when fed simple or complex diets containing no spray-dried plasma. Domestic Animal Endocrinology 24, 353–365.CrossRefGoogle ScholarPubMed
Guo, YM, Xia, ZG, Chen, SYYuan, JM 2003. Effects of dietary polyunsaturated fatty acids on antibody production and lymphocyte proliferation of laying hens. Asian-Australasian Journal of Animal Sciences 16, 1320–1325.Google Scholar
Guo, YM, Chen, SY, Xia, ZGYuan, JM 2004. Effects of different types of polyunsaturated fatty acids on immune function and PGE2 synthesis by peripheral blood leukocytes of laying hens. Animal Feed Science and Technology 116, 249–257.CrossRefGoogle Scholar
Halloy, DJ, Bouhet, S, Oswald, IP, Goret-Nicaise, M, Kobisch, M, Mainil, JGustin, PG 2004. Pathophysiological changes occurring during E. coli endotoxin and Pasteurella multocida challenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions. Veterinary Research 35, 309–324.CrossRefGoogle Scholar
Hayek, MG, Han, SN, Wu, D, Watkins, BA, Meydani, M, Dorsey, JL, Smith, DEMeydani, SN 1999. Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. Journal of Nutrition 129, 32–38.CrossRefGoogle Scholar
Johnson, RW 1997. Inhibition of growth by pro-inflammatory cytokines: an integrated view. Journal of Animal Science 75, 1244–1255.CrossRefGoogle ScholarPubMed
Johnson, RWvon Borel, E 1994. Lipopolysaccharide-induced sickness behavior in pigs is inhibited by pretreatment with indomethacin. Journal of Animal Science 72, 309–314.CrossRefGoogle ScholarPubMed
Karin, MGreten, FR 2005. NFκB: Linking inflammation and immunity to cancer development and progression. Nature Reviews Immunology 5, 749–759.CrossRefGoogle ScholarPubMed
Kastner, CJakse, G 2003. Measurement of immunoglobulins in seminal fluid with modified nephelometry-an alternative diagnostic tool for chronic prostatitis. Prostate Cancer and Prostatic Diseases 6, 86–89.CrossRefGoogle ScholarPubMed
Klasing, KCKorver, DR 1997. Leukocytic cytokines regulate growth rate and composition following activation of the immune system. Journal of Animal Science 75, 58–67.Google Scholar
Korver, DRKlasing, KC 1997. Dietary fish oil alters specific and inflammatory immune responses in chicks. Journal of Nutrition 127, 2039–2046.CrossRefGoogle ScholarPubMed
Korver, DR, Wakenell, PKlasing, KC 1997. Effects of fish oil or lofrin, a 5-lipoxygenase inhibitor, decrease the growth-suppressing effects of coccidiosis in broiler chicks. Poultry Science 76, 1355–1363.CrossRefGoogle ScholarPubMed
Korver, DR, Roura, EKlasing, KC 1998. Effects of dietary energy and oil source on broiler performance and response to an inflammatory challenge. Poultry Science 77, 1217–1227.CrossRefGoogle Scholar
Lai, CH, Yin, JD, Li, DF, Zhao, LD, Qiao, SYXing, JJ 2005a. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide. Journal of Nutrition 135, 239–244.Google Scholar
Lai, CH, Yin, JD, Li, DF, Zhao, LD, Qiao, SYChen, XJ 2005b. Effects of dietary conjugated linoleic acid supplementation on performance and immune function of weaned pigs. Archives of Animal Nutrition 59, 41–51.CrossRefGoogle ScholarPubMed
Liu, YL, Li, DF, Gong, LM, Yi, GF, Gaines, AMCarroll, JA 2003. Effects of fish oil supplementation on the performance and the immunological, adrenal, and somatotropic responses of weaned pigs after an E. coli lipopolysaccharide challenge. Journal of Animal Science 81, 2758–2765.CrossRefGoogle Scholar
Lo, CJ, Chiu, KC, Fu, M, Lo, RHelton, S 1998. Fish oil decreases macrophage tumor necrosis factor gene transcription by altering the NF-κB activity. Journal of Surgical Research 82, 216–221.CrossRefGoogle Scholar
Puertollano, MA, Puertollano, E, Ruiz-Bravo, A, Jiménez-Valera, M, De Pablo, MADe Cienfuegos, GÁ 2004. Changes in the immune functions and susceptibility to Listeria monocytogenes infection in mice fed dietary lipids. Immunology and Cell Biology 82, 370–376.CrossRefGoogle ScholarPubMed
Revajová, V, Pistl, J, Kaštel, R, Bindas, L’, Magic, SD, Levkut, M, Bomba, AŠajbidor, J 2001. Influencing the Immune parameters in germ-free piglets by administration of seal oil with increased content of ω-3 PUFA. Archiv für Tierernährung 54, 315–327.CrossRefGoogle ScholarPubMed
Sheu, BC, Hsu, SM, Lin, RH, Torng, PLHuang, SC 1999. Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma. Cancer 86, 1537–1543.3.0.CO;2-D>CrossRefGoogle ScholarPubMed
Sijben, JW, Schrama, JW, Parmentier, HK, van der Poel, JJKlasing, KC 2001. Effects of dietary polyunsaturated fatty acids on in vivo splenic cytokine mRNA expression in layer chicks immunized with Salmonella typhimurium lipopolysaccharide. Poultry Science 80, 1164–1170.CrossRefGoogle ScholarPubMed
Sijben, JWC, Klasing, KC, Schrama, JW, Parmentier, HK, Van der Poel, JJ, Savelkoul, HFJKaiser, P 2003. Early in vivo cytokine genes expression in chickens after challenge with Salmonella typhimurium lipopolysaccharide and modulation by dietary n-3 polyunsaturated fatty acids. Developmental and Comparative Immunology 27, 611–619.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute 2001. SAS software 8.02. SAS Inst. Inc., Cary, NC.Google Scholar
Takahashi, K, Kawamata, K, Akiba, Y, Iwata, TKasai, M 2002. Influence of dietary conjugated linoleic acid isomers on early inflammatory responses in male broiler chickens. British Poultry Science 43, 47–53.CrossRefGoogle ScholarPubMed
Wallace, FA, Miles, EACalder, PC 2003. Comparison of the effects of linseed oil and different doses of fish oil on mononuclear cell function in healthy human subjects. British Journal of Nutrition 89, 679–689.CrossRefGoogle ScholarPubMed
Warren, EJ, Finck, BN, Arkins, S, Kelley, KW, Scamurra, RW, Murtaugh, MPJohnson, RW 1997. Coincidental changes in behavior and plasma cortisol in unrestrained pigs after intracerebroventricular injection of tumor necrosis factor-α. Endocrinology 138, 2365–2371.CrossRefGoogle ScholarPubMed
Webel, DM, Finck, BN, Baker, DHJohnson, RW 1997. Time course of increased plasma cytokines, cortisol, and urea nitrogen in pigs following intraperitoneal injection of lipopolysaccharide. Journal of Animal Science 75, 1514–1520.CrossRefGoogle ScholarPubMed